BacMap

Definition	Azoarcus sp. BH72 chromosome, complete genome.
Accession	NC_008702
Length	4,376,040

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The map label for this gene is paaF [H]

Identifier: 119898218

GI number: 119898218

Start: 2116100

End: 2116894

Strand: Reverse

Name: paaF [H]

Synonym: azo1927

Alternate gene names: 119898218

Gene position: 2116894-2116100 (Counterclockwise)

Preceding gene: 119898219

Following gene: 119898215

Centisome position: 48.37

GC content: 69.69

Gene sequence:

>795_bases
ATGACAGACAAGGCTTACCTGCCCGGTGAGGGCGTGATCGTGGCCGGCCCCGATGCCGGCGTACTCGAAATCCGGCTGGA
TCGTCCGCAGGCGAAGAACGCATTGTCGACGCCGCTGCTGCGCAGCATCGTCGCGCTGCTGGAGCAGGCGGAACACGACG
ACGCGGTGCGCTGCATCGTGCTCACCGGCGGTGATCAGGTGTTCGCCGCCGGCGCCGACCTGGCCGAGATGGCCGCCAAG
GACATGCAGGCGGTGCTGCTGGAAGAGCGGCCCCGGCTGTTCGGCGCGATCGCGCGCTTTCCCAAGCCCATCATCGCCGC
CGTGTGCGGCTATGCGCTGGGCGGCGGTTGCGAGCTGGTGATGCATGCCGACATCGTCATCGCCGGCGAGTCGGCGCAGT
TCGGTCAGCCCGAAATCAATCTCGGCATCATCCCCGGCGCCGGCGGCACCCAGCGCCTGACCCGCGCGGTCGGCAAGTCG
GTGGCGATGAAGCTGGTGCTGGCGGGCGAGTTCATCCCGGCGCAGGAAGCGCGCGCCGCCGGGCTGGTGGCGGAAGTGGT
GGCCGACGAGGCCTGCATCGCACGCGCCCACGAGCTGGCCGGCAAGATCGCCAAGAAGGCACCGCTGGCGGTGCGGCTGG
CCAAGGATTCGGTGCTGCAGGCCTTCGAGACGCCGCTCGCCGCCGGGCTGGCGATCGAGCGCCGCAACTTCGTGGTGCTC
GCCGGCACCGAGGACCGCAACGAGGGCGTCAAATCCTTCCTGGAAAAGCGCAAGCCGGTCTGGAAGGGCCGCTGA

Upstream 100 bases:

>100_bases
ATTTCCCGGCGATGACCGCCGTCCAGGTTGCAGACCTCGTCGAGGACGAGGCCAAGGTCGAGATCGAGGTCACCGCCGTC
ATTCCAGACTGAGAGGAAGG

Downstream 100 bases:

>100_bases
GTACCCCCGCGGCGGGGGTGCGACGGGCGTAACGCACGCCTGTCCCCCCGCCGCGCTTACTGCGGCTGGCGATCGGCCAG
CCGGTCGATATCGGCCACGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTDKAYLPGEGVIVAGPDAGVLEIRLDRPQAKNALSTPLLRSIVALLEQAEHDDAVRCIVLTGGDQVFAAGADLAEMAAK
DMQAVLLEERPRLFGAIARFPKPIIAAVCGYALGGGCELVMHADIVIAGESAQFGQPEINLGIIPGAGGTQRLTRAVGKS
VAMKLVLAGEFIPAQEARAAGLVAEVVADEACIARAHELAGKIAKKAPLAVRLAKDSVLQAFETPLAAGLAIERRNFVVL
AGTEDRNEGVKSFLEKRKPVWKGR

Sequences:

>Translated_264_residues
MTDKAYLPGEGVIVAGPDAGVLEIRLDRPQAKNALSTPLLRSIVALLEQAEHDDAVRCIVLTGGDQVFAAGADLAEMAAK
DMQAVLLEERPRLFGAIARFPKPIIAAVCGYALGGGCELVMHADIVIAGESAQFGQPEINLGIIPGAGGTQRLTRAVGKS
VAMKLVLAGEFIPAQEARAAGLVAEVVADEACIARAHELAGKIAKKAPLAVRLAKDSVLQAFETPLAAGLAIERRNFVVL
AGTEDRNEGVKSFLEKRKPVWKGR
>Mature_263_residues
TDKAYLPGEGVIVAGPDAGVLEIRLDRPQAKNALSTPLLRSIVALLEQAEHDDAVRCIVLTGGDQVFAAGADLAEMAAKD
MQAVLLEERPRLFGAIARFPKPIIAAVCGYALGGGCELVMHADIVIAGESAQFGQPEINLGIIPGAGGTQRLTRAVGKSV
AMKLVLAGEFIPAQEARAAGLVAEVVADEACIARAHELAGKIAKKAPLAVRLAKDSVLQAFETPLAAGLAIERRNFVVLA
GTEDRNEGVKSFLEKRKPVWKGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=48.1927710843374, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=37.1541501976285, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI150378541, Length=259, Percent_Identity=33.976833976834, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI70995211, Length=271, Percent_Identity=29.1512915129151, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI68989263, Length=207, Percent_Identity=36.231884057971, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI260274832, Length=227, Percent_Identity=31.7180616740088, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI45643119, Length=227, Percent_Identity=31.7180616740088, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI260275230, Length=227, Percent_Identity=31.7180616740088, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=36.2637362637363, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI37594471, Length=207, Percent_Identity=30.9178743961353, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI37594469, Length=207, Percent_Identity=30.9178743961353, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757966, Length=181, Percent_Identity=29.2817679558011, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538581, Length=181, Percent_Identity=29.2817679558011, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI25453481, Length=181, Percent_Identity=29.2817679558011, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538573, Length=181, Percent_Identity=29.2817679558011, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757968, Length=181, Percent_Identity=29.8342541436464, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI49355787, Length=181, Percent_Identity=29.8342541436464, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI221307494, Length=200, Percent_Identity=28.5, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI221136756, Length=200, Percent_Identity=28.5, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221136753, Length=200, Percent_Identity=28.5, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI62530384, Length=263, Percent_Identity=25.8555133079848, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI116235487, Length=182, Percent_Identity=28.021978021978, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=244, Percent_Identity=53.6885245901639, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=34.9019607843137, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=35.1351351351351, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87082183, Length=246, Percent_Identity=30.8943089430894, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=30.8943089430894, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=31.5789473684211, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=33.8383838383838, Blast_Score=88, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=45.3815261044177, Blast_Score=217, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI25145438, Length=238, Percent_Identity=45.7983193277311, Blast_Score=202, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=33.6206896551724, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=244, Percent_Identity=29.0983606557377, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=31.6037735849057, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=243, Percent_Identity=25.1028806584362, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=172, Percent_Identity=33.7209302325581, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=171, Percent_Identity=32.7485380116959, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17508953, Length=171, Percent_Identity=32.7485380116959, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508951, Length=171, Percent_Identity=32.7485380116959, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=228, Percent_Identity=27.6315789473684, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=224, Percent_Identity=25.8928571428571, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=175, Percent_Identity=29.7142857142857, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=175, Percent_Identity=29.7142857142857, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=204, Percent_Identity=27.9411764705882, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=208, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=180, Percent_Identity=28.3333333333333, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=48.7704918032787, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=48.7704918032787, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=38.28125, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=27.027027027027, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19920382, Length=265, Percent_Identity=30.5660377358491, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24650670, Length=252, Percent_Identity=29.3650793650794, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357171, Length=252, Percent_Identity=27.7777777777778, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24654903, Length=200, Percent_Identity=26.5, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571729, Length=172, Percent_Identity=29.6511627906977, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571730, Length=172, Percent_Identity=29.6511627906977, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583077, Length=171, Percent_Identity=34.5029239766082, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583079, Length=171, Percent_Identity=34.5029239766082, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921000, Length=171, Percent_Identity=34.5029239766082, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27716; Mature: 27584

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKAYLPGEGVIVAGPDAGVLEIRLDRPQAKNALSTPLLRSIVALLEQAEHDDAVRCIV
CCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LTGGDQVFAAGADLAEMAAKDMQAVLLEERPRLFGAIARFPKPIIAAVCGYALGGGCELV
EECCCEEEECCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEE
MHADIVIAGESAQFGQPEINLGIIPGAGGTQRLTRAVGKSVAMKLVLAGEFIPAQEARAA
EEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GLVAEVVADEACIARAHELAGKIAKKAPLAVRLAKDSVLQAFETPLAAGLAIERRNFVVL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCEEEECCCEEEE
AGTEDRNEGVKSFLEKRKPVWKGR
ECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDKAYLPGEGVIVAGPDAGVLEIRLDRPQAKNALSTPLLRSIVALLEQAEHDDAVRCIV
CCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LTGGDQVFAAGADLAEMAAKDMQAVLLEERPRLFGAIARFPKPIIAAVCGYALGGGCELV
EECCCEEEECCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEE
MHADIVIAGESAQFGQPEINLGIIPGAGGTQRLTRAVGKSVAMKLVLAGEFIPAQEARAA
EEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GLVAEVVADEACIARAHELAGKIAKKAPLAVRLAKDSVLQAFETPLAAGLAIERRNFVVL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCEEEECCCEEEE
AGTEDRNEGVKSFLEKRKPVWKGR
ECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]