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The map label for this gene is gcvA [H]

Identifier: 119898216

GI number: 119898216

Start: 2114231

End: 2115175

Strand: Direct

Name: gcvA [H]

Synonym: azo1925

Alternate gene names: 119898216

Gene position: 2114231-2115175 (Clockwise)

Preceding gene: 119898210

Following gene: 119898217

Centisome position: 48.31

GC content: 67.3

Gene sequence:

>945_bases
ATGCGCCGAAAAATTCCGAGCACCGCCGCGCTGCAGGCCTTCGATGCCGCCGCGCGCCACGAGAGTTTCACCCACGCCGC
GGACGAGCTTGCGCTGACGCAGAGCGCGATCTGCCGCCAGATCGCCGGGTTGGAGGATTTCCTCGGTGTCGCCCTGTTCC
GCCGCACCCGGCGCGGCGTGCGGCTGACCGAGGCGGGGCTCAACTACAGCCGCAAGGTCGCAGCCCGGCTGGACGCGGTG
GAGCGCGACACGCTGGCGGTGATGGCGCAGCGCGACAGTGGCGCCACGCTGGAAATCGCGACGGTGCCCACCTTCGCCAC
CCGATGGCTGTTGCCGCGGCTGCCGGGGTTTTTGGCGCAGCACCCGGATGTCACGATCAATCTCAGCACCCGCACGCGCC
CTTTCCTGTTCGACGAAACCGAGTTCGATGCCGCGATCTACTTCGGCGACGCCGGGTGGCCCGGCACCGAGGCGCACTTC
CTGATGCGCGAGTATCCCGTGCCGGTGTGCAGCCCGCTGCTATCCGGCGCCAAGCCGAGCATGAGCCCGGCGGAAATCGC
CCAGCTGCCGCTGCTGCAGCAGACCACCCGGCCGTATGCGTGGCGGCACTGGTTCGAATCGCTCGGCATGAGCATTTCGC
AAGACATGAGCGGCCCGCGGCTGGAGCTGTTCTCGATGCTGGCCGAAGCTGCGATCCAGCGCCTGGGCGTGGCGCTGATA
CCGCCCTTCCTGATCCGTCACGAACTCGACGCGGGCCTGCTGATCACGCCCACGCCGCACGCCCAGCGCAGTGAGCGGGC
CTATTACTTCATCGTGCCGGAACGCAAGTCGCAGCATGCCGCGCTCGGCGCCTTTCGCGCCTGGCTGGTCGAGGAGACCG
CGCGTTATGCAGAAAACGAGAATTCCTCCGCATATTCAGGCAAGGACGACGTCAATCCTGCATGA

Upstream 100 bases:

>100_bases
AACGAGTATTTGGAAGTAAACTGTGCGCTTTACTCACTCCGAAGCGACCGTAACGGGCTTCTTTGCTGGTGTGGCCCGCG
GACGGAATGCGAACAGAGCA

Downstream 100 bases:

>100_bases
CGGTTGCACTATGCTTGCGGACTTTGCCGCGGCGCTTCCACTGGCGCAGCCGGCCACGAGAATCCGCCCCTCCACGGCGG
ACGCAAGATGGAGTGTGGAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRRKIPSTAALQAFDAAARHESFTHAADELALTQSAICRQIAGLEDFLGVALFRRTRRGVRLTEAGLNYSRKVAARLDAV
ERDTLAVMAQRDSGATLEIATVPTFATRWLLPRLPGFLAQHPDVTINLSTRTRPFLFDETEFDAAIYFGDAGWPGTEAHF
LMREYPVPVCSPLLSGAKPSMSPAEIAQLPLLQQTTRPYAWRHWFESLGMSISQDMSGPRLELFSMLAEAAIQRLGVALI
PPFLIRHELDAGLLITPTPHAQRSERAYYFIVPERKSQHAALGAFRAWLVEETARYAENENSSAYSGKDDVNPA

Sequences:

>Translated_314_residues
MRRKIPSTAALQAFDAAARHESFTHAADELALTQSAICRQIAGLEDFLGVALFRRTRRGVRLTEAGLNYSRKVAARLDAV
ERDTLAVMAQRDSGATLEIATVPTFATRWLLPRLPGFLAQHPDVTINLSTRTRPFLFDETEFDAAIYFGDAGWPGTEAHF
LMREYPVPVCSPLLSGAKPSMSPAEIAQLPLLQQTTRPYAWRHWFESLGMSISQDMSGPRLELFSMLAEAAIQRLGVALI
PPFLIRHELDAGLLITPTPHAQRSERAYYFIVPERKSQHAALGAFRAWLVEETARYAENENSSAYSGKDDVNPA
>Mature_314_residues
MRRKIPSTAALQAFDAAARHESFTHAADELALTQSAICRQIAGLEDFLGVALFRRTRRGVRLTEAGLNYSRKVAARLDAV
ERDTLAVMAQRDSGATLEIATVPTFATRWLLPRLPGFLAQHPDVTINLSTRTRPFLFDETEFDAAIYFGDAGWPGTEAHF
LMREYPVPVCSPLLSGAKPSMSPAEIAQLPLLQQTTRPYAWRHWFESLGMSISQDMSGPRLELFSMLAEAAIQRLGVALI
PPFLIRHELDAGLLITPTPHAQRSERAYYFIVPERKSQHAALGAFRAWLVEETARYAENENSSAYSGKDDVNPA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=304, Percent_Identity=37.5, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=33.6842105263158, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=29.1095890410959, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=29.4701986754967, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI157672245, Length=261, Percent_Identity=32.183908045977, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787128, Length=135, Percent_Identity=35.5555555555556, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786401, Length=167, Percent_Identity=28.7425149700599, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787589, Length=151, Percent_Identity=33.7748344370861, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34911; Mature: 34911

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKIPSTAALQAFDAAARHESFTHAADELALTQSAICRQIAGLEDFLGVALFRRTRRGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
RLTEAGLNYSRKVAARLDAVERDTLAVMAQRDSGATLEIATVPTFATRWLLPRLPGFLAQ
EEEHHCCCHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCHHHHHHHHHHCCHHHHC
HPDVTINLSTRTRPFLFDETEFDAAIYFGDAGWPGTEAHFLMREYPVPVCSPLLSGAKPS
CCCEEEEECCCCCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCC
MSPAEIAQLPLLQQTTRPYAWRHWFESLGMSISQDMSGPRLELFSMLAEAAIQRLGVALI
CCHHHHHHCCHHHHHCCCHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHCCHHHC
PPFLIRHELDAGLLITPTPHAQRSERAYYFIVPERKSQHAALGAFRAWLVEETARYAENE
CHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
NSSAYSGKDDVNPA
CCCCCCCCCCCCCC
>Mature Secondary Structure
MRRKIPSTAALQAFDAAARHESFTHAADELALTQSAICRQIAGLEDFLGVALFRRTRRGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
RLTEAGLNYSRKVAARLDAVERDTLAVMAQRDSGATLEIATVPTFATRWLLPRLPGFLAQ
EEEHHCCCHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCHHHHHHHHHHCCHHHHC
HPDVTINLSTRTRPFLFDETEFDAAIYFGDAGWPGTEAHFLMREYPVPVCSPLLSGAKPS
CCCEEEEECCCCCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCC
MSPAEIAQLPLLQQTTRPYAWRHWFESLGMSISQDMSGPRLELFSMLAEAAIQRLGVALI
CCHHHHHHCCHHHHHCCCHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHCCHHHC
PPFLIRHELDAGLLITPTPHAQRSERAYYFIVPERKSQHAALGAFRAWLVEETARYAENE
CHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
NSSAYSGKDDVNPA
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]