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The map label for this gene is glgX [H]

Identifier: 119898085

GI number: 119898085

Start: 1962504

End: 1964684

Strand: Direct

Name: glgX [H]

Synonym: azo1794

Alternate gene names: 119898085

Gene position: 1962504-1964684 (Clockwise)

Preceding gene: 119898084

Following gene: 119898086

Centisome position: 44.85

GC content: 71.76

Gene sequence:

>2181_bases
ATGACGGCCGCGCTGCAGATGACGCTCGAAGCCGGCCAGGCCTATCCGCTGGGGGCAACCTGGACCGGCGACGGGGTCAA
CTTCGCCGTGTTCTCGGCCCACGCCAGCCGCATGGAGCTGTGCCTGTTCGACGAGGCCGGGCGCAACGAGATCGCGCGCA
TGGACCTGCCCGAACTCACCGACGAGGTCTGGCACGGCTTTCTGCCGGGCGCGGGGCCGGGGCTGGTGTACGGCTACCGC
GCGCACGGGCCCTACGTGCCCGAGGAAGGCCACCGCTTCAACCCGCACAAGCTGCTGCTCGACCCCTACGCCACCGAGCT
GGTCGGCCGCCTGCGCTGGGGGCCGCCGATCTTCGGCTACGAGCTGCGCAACAAGCGCCCGGGGCACGGCTTCGACAAGC
GCGACAGCGCCGCCTGCACGCTCAAGGCGCGTGTCGCCGCGCCCTGGCCGAGCGCCAGCCGCCCGGCGCGGCGCGGTCAG
CGCGTGCCGTGGGAATGCATGGTGATCTATGAGGCGCATGTACGCGGCCTCACCCGCCAGCATCCGGCGGTGGGCGCGGA
GCACCGCGGCACCATCGCGGGGCTGGGCAGCGCGGCGGTCATCGACCACCTCAAGGCCCTGGGCGTGACCTCGGTGGAGC
TGCTGCCGGTGCACGCCTACGTGGATGACGCCTACCTGACCGACAAGGGCCTGCGCAACTACTGGGGCTACAACTCGATC
GGCTTCTTCGCGCTGGAGCCGCGCTACCTCGCCGGCGAGGGCGCGGCGGAATTCCGCGACACCGTAGCGCGGCTGCACGA
CGCCGGCCTCGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAGGGCAACGAGCTGGGCCCCACGCTCAGCTTCA
AGGGCCTGGACAACGCCTCCTACTACCGCCTCAACCCGGACGACCGGCGCTACTACATCAACGACACCGGCACCGGCAAC
ACGGTGAATCTCAGCCATCCGCGCGTGCTGCAGCTGGTGATGGACAGCCTGCGCTACTGGGTGACGCAGATGGAGGTGGA
CGGCTTCCGTTTCGACCTCGCCACCATCCTCGGGCGCGAGGCGGCAGGCTTCGACCCCGGCTGCGGCTTCTTCGACGCCG
TGCGGCAGGACCCGGTGCTCAACCGCGTCAAGCTGATCGCCGAGCCTTGGGACTGCGGCCCCGGCGGCTACCAGCCGGGC
AATTTCCCGCCCGGGTGGGCGGAATGGAACGACGGCTTCCGCGACACCGTGCGCGCCTTCTGGCGCGGTGACGAAGGCCA
GGCGCCCGGCCTCGCGACCCGGCTCGCCGGCTCGGCCGACCGCTTCAACCACCACGGCCGGCGGCCGTGGGCCAGCATCA
ACTTCATCACCGCGCACGACGGGTTCACGCTGCACGACCTCGTCTCGTACAACGACAAGCACAACGAGGCCAACGGCGAG
GACAACCGCGACGGCCACGACGACAACCGGTCGTGGAACTGCGGCGCGGAAGGCCCCACCGACGACCCCGAGGTGCTGGC
GTTGCGCGAGCGCCAGAAGCGCAACCTGCTCGCCACGCTGCTGCTCGCGCAGGGCACGCCGATGCTGCTGGCCGGCGACG
AGATCGGCCGCAGCCAGCAGGGCAACAACAACGCCTATTGCCAGGACAACGAGATCAGCTGGGTGCACTGGGCCGGCGTG
GATGCCGACGGCGAGCGCCTGCGCGCCTTCGTGCGCAGCCTGCTGCTGATCCGCCGCGCGCTGCCGGTGCTGCGCCCGGC
GCGCTTCGCCACCGGCGGCGACCCCGGCCACGTGCTGCGCGACGTGATCTGGCTGGCGCCCAACGGCGTCGAACTGACGC
CCGAGGGCTGGGCGGACGCGCAGATGCGCTGCTTTGCGATGCTGGCCGACGGCCGCGCCCACCTCGGCACCGGGCCGGAA
CCCGCCGCCGACGCGGTCGTGCTGGTGGTGCTGAACGCCGCGCACGAGGCGGTCGAGTGCACCTTGCCGCAGGCGGCCGG
CATCACCCGCTGGGTGCGGCTGGTGGATACCGCGCAGGCAGAGGTGGCGGATTTCGCCGAGTCCGCGCCCGGCGAGGGCT
ACAGCGCCGCCGGGCGCTCGCTGCAGCTTTTCGTCGCCGCACCCACGCCGCAGGCGGCCGAATGCGTTGCGCGCCTGACC
GCGCGGCTGGGCGAGGGCTAG

Upstream 100 bases:

>100_bases
CCTATCCCTTCGTGATGGCCACCACGGTGCTCGACAAGCTCGGCTTCGTGCACCGCGTCATCACCGAGGCCGCGGCCGCC
CGGCGCCGCGGGACGGACGC

Downstream 100 bases:

>100_bases
GCCATGGCCACCGTCTCCACCGCAGAACTGCAAAGCCGCTACCTGCGCCGCGAACCGCTGGCCGAAGGCGGCTTCCGCTA
TCTGCGCCCGGACGGCCGGC

Product: putative glycogen debranching enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MTAALQMTLEAGQAYPLGATWTGDGVNFAVFSAHASRMELCLFDEAGRNEIARMDLPELTDEVWHGFLPGAGPGLVYGYR
AHGPYVPEEGHRFNPHKLLLDPYATELVGRLRWGPPIFGYELRNKRPGHGFDKRDSAACTLKARVAAPWPSASRPARRGQ
RVPWECMVIYEAHVRGLTRQHPAVGAEHRGTIAGLGSAAVIDHLKALGVTSVELLPVHAYVDDAYLTDKGLRNYWGYNSI
GFFALEPRYLAGEGAAEFRDTVARLHDAGLEVILDVVYNHTAEGNELGPTLSFKGLDNASYYRLNPDDRRYYINDTGTGN
TVNLSHPRVLQLVMDSLRYWVTQMEVDGFRFDLATILGREAAGFDPGCGFFDAVRQDPVLNRVKLIAEPWDCGPGGYQPG
NFPPGWAEWNDGFRDTVRAFWRGDEGQAPGLATRLAGSADRFNHHGRRPWASINFITAHDGFTLHDLVSYNDKHNEANGE
DNRDGHDDNRSWNCGAEGPTDDPEVLALRERQKRNLLATLLLAQGTPMLLAGDEIGRSQQGNNNAYCQDNEISWVHWAGV
DADGERLRAFVRSLLLIRRALPVLRPARFATGGDPGHVLRDVIWLAPNGVELTPEGWADAQMRCFAMLADGRAHLGTGPE
PAADAVVLVVLNAAHEAVECTLPQAAGITRWVRLVDTAQAEVADFAESAPGEGYSAAGRSLQLFVAAPTPQAAECVARLT
ARLGEG

Sequences:

>Translated_726_residues
MTAALQMTLEAGQAYPLGATWTGDGVNFAVFSAHASRMELCLFDEAGRNEIARMDLPELTDEVWHGFLPGAGPGLVYGYR
AHGPYVPEEGHRFNPHKLLLDPYATELVGRLRWGPPIFGYELRNKRPGHGFDKRDSAACTLKARVAAPWPSASRPARRGQ
RVPWECMVIYEAHVRGLTRQHPAVGAEHRGTIAGLGSAAVIDHLKALGVTSVELLPVHAYVDDAYLTDKGLRNYWGYNSI
GFFALEPRYLAGEGAAEFRDTVARLHDAGLEVILDVVYNHTAEGNELGPTLSFKGLDNASYYRLNPDDRRYYINDTGTGN
TVNLSHPRVLQLVMDSLRYWVTQMEVDGFRFDLATILGREAAGFDPGCGFFDAVRQDPVLNRVKLIAEPWDCGPGGYQPG
NFPPGWAEWNDGFRDTVRAFWRGDEGQAPGLATRLAGSADRFNHHGRRPWASINFITAHDGFTLHDLVSYNDKHNEANGE
DNRDGHDDNRSWNCGAEGPTDDPEVLALRERQKRNLLATLLLAQGTPMLLAGDEIGRSQQGNNNAYCQDNEISWVHWAGV
DADGERLRAFVRSLLLIRRALPVLRPARFATGGDPGHVLRDVIWLAPNGVELTPEGWADAQMRCFAMLADGRAHLGTGPE
PAADAVVLVVLNAAHEAVECTLPQAAGITRWVRLVDTAQAEVADFAESAPGEGYSAAGRSLQLFVAAPTPQAAECVARLT
ARLGEG
>Mature_725_residues
TAALQMTLEAGQAYPLGATWTGDGVNFAVFSAHASRMELCLFDEAGRNEIARMDLPELTDEVWHGFLPGAGPGLVYGYRA
HGPYVPEEGHRFNPHKLLLDPYATELVGRLRWGPPIFGYELRNKRPGHGFDKRDSAACTLKARVAAPWPSASRPARRGQR
VPWECMVIYEAHVRGLTRQHPAVGAEHRGTIAGLGSAAVIDHLKALGVTSVELLPVHAYVDDAYLTDKGLRNYWGYNSIG
FFALEPRYLAGEGAAEFRDTVARLHDAGLEVILDVVYNHTAEGNELGPTLSFKGLDNASYYRLNPDDRRYYINDTGTGNT
VNLSHPRVLQLVMDSLRYWVTQMEVDGFRFDLATILGREAAGFDPGCGFFDAVRQDPVLNRVKLIAEPWDCGPGGYQPGN
FPPGWAEWNDGFRDTVRAFWRGDEGQAPGLATRLAGSADRFNHHGRRPWASINFITAHDGFTLHDLVSYNDKHNEANGED
NRDGHDDNRSWNCGAEGPTDDPEVLALRERQKRNLLATLLLAQGTPMLLAGDEIGRSQQGNNNAYCQDNEISWVHWAGVD
ADGERLRAFVRSLLLIRRALPVLRPARFATGGDPGHVLRDVIWLAPNGVELTPEGWADAQMRCFAMLADGRAHLGTGPEP
AADAVVLVVLNAAHEAVECTLPQAAGITRWVRLVDTAQAEVADFAESAPGEGYSAAGRSLQLFVAAPTPQAAECVARLTA
RLGEG

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=661, Percent_Identity=46.4447806354009, Blast_Score=543, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79531; Mature: 79400

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAALQMTLEAGQAYPLGATWTGDGVNFAVFSAHASRMELCLFDEAGRNEIARMDLPELT
CCCEEEEEECCCCCCCCCCEECCCCCEEEEEECCCCCEEEEEECCCCCCCHHHCCCHHHH
DEVWHGFLPGAGPGLVYGYRAHGPYVPEEGHRFNPHKLLLDPYATELVGRLRWGPPIFGY
HHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCEECE
ELRNKRPGHGFDKRDSAACTLKARVAAPWPSASRPARRGQRVPWECMVIYEAHVRGLTRQ
ECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHCCCCCCEEEEEEEEHHHHCHHHC
HPAVGAEHRGTIAGLGSAAVIDHLKALGVTSVELLPVHAYVDDAYLTDKGLRNYWGYNSI
CCCCCCCCCCCEECCCHHHHHHHHHHCCCCEEEEEEEEEEECCCHHCCHHHHHHCCCCCC
GFFALEPRYLAGEGAAEFRDTVARLHDAGLEVILDVVYNHTAEGNELGPTLSFKGLDNAS
EEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
YYRLNPDDRRYYINDTGTGNTVNLSHPRVLQLVMDSLRYWVTQMEVDGFRFDLATILGRE
EEEECCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCC
AAGFDPGCGFFDAVRQDPVLNRVKLIAEPWDCGPGGYQPGNFPPGWAEWNDGFRDTVRAF
CCCCCCCCCHHHHHHCCHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
WRGDEGQAPGLATRLAGSADRFNHHGRRPWASINFITAHDGFTLHDLVSYNDKHNEANGE
HCCCCCCCCCHHHHHCCCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCC
DNRDGHDDNRSWNCGAEGPTDDPEVLALRERQKRNLLATLLLAQGTPMLLAGDEIGRSQQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCCC
GNNNAYCQDNEISWVHWAGVDADGERLRAFVRSLLLIRRALPVLRPARFATGGDPGHVLR
CCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
DVIWLAPNGVELTPEGWADAQMRCFAMLADGRAHLGTGPEPAADAVVLVVLNAAHEAVEC
HHHHCCCCCCEECCCCCCCHHHEEEEEEECCCCCCCCCCCCHHCEEEEEEEHHCHHHHEE
TLPQAAGITRWVRLVDTAQAEVADFAESAPGEGYSAAGRSLQLFVAAPTPQAAECVARLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCCCHHHHHHHHHH
ARLGEG
HHHCCC
>Mature Secondary Structure 
TAALQMTLEAGQAYPLGATWTGDGVNFAVFSAHASRMELCLFDEAGRNEIARMDLPELT
CCEEEEEECCCCCCCCCCEECCCCCEEEEEECCCCCEEEEEECCCCCCCHHHCCCHHHH
DEVWHGFLPGAGPGLVYGYRAHGPYVPEEGHRFNPHKLLLDPYATELVGRLRWGPPIFGY
HHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCEECE
ELRNKRPGHGFDKRDSAACTLKARVAAPWPSASRPARRGQRVPWECMVIYEAHVRGLTRQ
ECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHCCCCCCEEEEEEEEHHHHCHHHC
HPAVGAEHRGTIAGLGSAAVIDHLKALGVTSVELLPVHAYVDDAYLTDKGLRNYWGYNSI
CCCCCCCCCCCEECCCHHHHHHHHHHCCCCEEEEEEEEEEECCCHHCCHHHHHHCCCCCC
GFFALEPRYLAGEGAAEFRDTVARLHDAGLEVILDVVYNHTAEGNELGPTLSFKGLDNAS
EEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
YYRLNPDDRRYYINDTGTGNTVNLSHPRVLQLVMDSLRYWVTQMEVDGFRFDLATILGRE
EEEECCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCC
AAGFDPGCGFFDAVRQDPVLNRVKLIAEPWDCGPGGYQPGNFPPGWAEWNDGFRDTVRAF
CCCCCCCCCHHHHHHCCHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
WRGDEGQAPGLATRLAGSADRFNHHGRRPWASINFITAHDGFTLHDLVSYNDKHNEANGE
HCCCCCCCCCHHHHHCCCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCC
DNRDGHDDNRSWNCGAEGPTDDPEVLALRERQKRNLLATLLLAQGTPMLLAGDEIGRSQQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCCC
GNNNAYCQDNEISWVHWAGVDADGERLRAFVRSLLLIRRALPVLRPARFATGGDPGHVLR
CCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
DVIWLAPNGVELTPEGWADAQMRCFAMLADGRAHLGTGPEPAADAVVLVVLNAAHEAVEC
HHHHCCCCCCEECCCCCCCHHHEEEEEEECCCCCCCCCCCCHHCEEEEEEEHHCHHHHEE
TLPQAAGITRWVRLVDTAQAEVADFAESAPGEGYSAAGRSLQLFVAAPTPQAAECVARLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCCCHHHHHHHHHH
ARLGEG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]