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The map label for this gene is crp [H]

Identifier: 119897969

GI number: 119897969

Start: 1843392

End: 1844072

Strand: Reverse

Name: crp [H]

Synonym: azo1678

Alternate gene names: 119897969

Gene position: 1844072-1843392 (Counterclockwise)

Preceding gene: 119897970

Following gene: 119897968

Centisome position: 42.14

GC content: 71.07

Gene sequence:

>681_bases
GTGAAGCCGGGCTCCGGTCATCCCGCCGGCGTCGCGCTGGACGTCGCCAACCATCCGCTGGGCGCGGCGCTCGGCGCCGA
CCAACCTTTCCAGACGCCCACCGTGCGCCACTACAGCCTGGGCAGCTTCATCGCCACCCCCGGCGCGGGCGGCAACACGG
TGTTCGTGCTGCAGCAGGGCCGGCTGCGGGTCTTCCTGTCCGGCGAGGACAAGGAGCTGACGCTGGCCTTCCTCGAACCC
GGCGAGGTCTATTGCACCCACACCCGTGCCTGGGTGGAGGCCGTCACCGACTGCACGCTGCTGATGGCCGATACCGCGCA
CTTCCAGGGCCGCCTGCTCGCGGACCCTACGCTGATGCCGGTCACGATCCGCGTCTTCGCGCAGCTGCTCGCGCGCTCGA
TCGACATCATCGAAAACCTCGCCTTCCGCGACGTCGCGGCGCGGCTTGCCAACTTCCTCCTCGGCCTCGCGCGCGGCGCA
GACCCCGCCCTGCCGGCCGGACTGCGGATACCGGTGCAGCTGCGCACCGAAGACATCGCCCGCCTCCTTGGCACCTCGCG
GCAGACGGTGTCGTCGCTGATCAACCAGCTGGTGCGCGAAGGCGTGATCGCGCGCGACGGCCGCCGCGCGGTGCTGCTGC
TGGACCCCGCGGAACTGAAACGCCGCGCGCAAGGCGACTGA

Upstream 100 bases:

>100_bases
GCCAATCTGATGGCGGCCCGCTACCAGGTACAGAAGTGGCTGGAGGCGCCGCGCGCATGAGGCCGGCGCGCTGTCGCACG
GCTGCGGCGGGCACGACACG

Downstream 100 bases:

>100_bases
ACGCGGCGCGTTTTGTCGTCTGGCTGACAGACGCGCGCCAAGGGCATCCGGACAATGCTGTCCGTCATCCACCCTTTTGC
GCCGCGACCCGCCATGAACG

Product: putative cyclic nucleotide-binding protein

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKPGSGHPAGVALDVANHPLGAALGADQPFQTPTVRHYSLGSFIATPGAGGNTVFVLQQGRLRVFLSGEDKELTLAFLEP
GEVYCTHTRAWVEAVTDCTLLMADTAHFQGRLLADPTLMPVTIRVFAQLLARSIDIIENLAFRDVAARLANFLLGLARGA
DPALPAGLRIPVQLRTEDIARLLGTSRQTVSSLINQLVREGVIARDGRRAVLLLDPAELKRRAQGD

Sequences:

>Translated_226_residues
MKPGSGHPAGVALDVANHPLGAALGADQPFQTPTVRHYSLGSFIATPGAGGNTVFVLQQGRLRVFLSGEDKELTLAFLEP
GEVYCTHTRAWVEAVTDCTLLMADTAHFQGRLLADPTLMPVTIRVFAQLLARSIDIIENLAFRDVAARLANFLLGLARGA
DPALPAGLRIPVQLRTEDIARLLGTSRQTVSSLINQLVREGVIARDGRRAVLLLDPAELKRRAQGD
>Mature_226_residues
MKPGSGHPAGVALDVANHPLGAALGADQPFQTPTVRHYSLGSFIATPGAGGNTVFVLQQGRLRVFLSGEDKELTLAFLEP
GEVYCTHTRAWVEAVTDCTLLMADTAHFQGRLLADPTLMPVTIRVFAQLLARSIDIIENLAFRDVAARLANFLLGLARGA
DPALPAGLRIPVQLRTEDIARLLGTSRQTVSSLINQLVREGVIARDGRRAVLLLDPAELKRRAQGD

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=191, Percent_Identity=26.1780104712042, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24275; Mature: 24275

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPGSGHPAGVALDVANHPLGAALGADQPFQTPTVRHYSLGSFIATPGAGGNTVFVLQQG
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCEEEEEECC
RLRVFLSGEDKELTLAFLEPGEVYCTHTRAWVEAVTDCTLLMADTAHFQGRLLADPTLMP
CEEEEEECCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHEEEECCCCCCCEEECCCCCHH
VTIRVFAQLLARSIDIIENLAFRDVAARLANFLLGLARGADPALPAGLRIPVQLRTEDIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHH
RLLGTSRQTVSSLINQLVREGVIARDGRRAVLLLDPAELKRRAQGD
HHHCCCHHHHHHHHHHHHHCCCEECCCCEEEEEECHHHHHHHCCCC
>Mature Secondary Structure
MKPGSGHPAGVALDVANHPLGAALGADQPFQTPTVRHYSLGSFIATPGAGGNTVFVLQQG
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCEEEEEECC
RLRVFLSGEDKELTLAFLEPGEVYCTHTRAWVEAVTDCTLLMADTAHFQGRLLADPTLMP
CEEEEEECCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHEEEECCCCCCCEEECCCCCHH
VTIRVFAQLLARSIDIIENLAFRDVAARLANFLLGLARGADPALPAGLRIPVQLRTEDIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHH
RLLGTSRQTVSSLINQLVREGVIARDGRRAVLLLDPAELKRRAQGD
HHHCCCHHHHHHHHHHHHHCCCEECCCCEEEEEECHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]