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The map label for this gene is holB [H]

Identifier: 119897887

GI number: 119897887

Start: 1752207

End: 1753256

Strand: Direct

Name: holB [H]

Synonym: azo1596

Alternate gene names: 119897887

Gene position: 1752207-1753256 (Clockwise)

Preceding gene: 119897886

Following gene: 119897888

Centisome position: 40.04

GC content: 69.9

Gene sequence:

>1050_bases
ATGATCCATCCGTGGCTGCAGCCGGTCTGGTCGCGTCTGGTCGCGCTCGAAGGGCGCTTGCCGCACGCGCTGCTGTTCAT
CGGCCCGGCCGGCCTGGGCAAGCGTGATCTGGCCGAGATGCTGGCTGCGCGCCTGCTGTGCGACAGCCCGGCGGCCGACG
GTGCCGCCTGCGGCCGTTGCGCCGCCTGTGCCTGGCGGCTGGCAGGCAATCACCCGGACTTCTATCACCTGGTGCCGGCG
GCGGACGCCGAGAAGGAAGCCGCCGCCGAAGGCGGCGACGGCGAGGGCGGGACGACCAAGGCCAAGTCGGCGCAGATCGT
GATCGAACAGATCCGTGACCTGCAGGAGGCGCTGACGGTGACCGGCCACCACAGCGCACGCCGGGTGGTCGTCGTCGATC
CTGCCGAGGCGATGAACGTATTCACCGCCAATGCGCTGCTCAAGCTCTTGGAGGAACCGCCCGCGGGCTGCACCTTCGTG
CTGGTGTCCTCGGCGCCGCGCCGCCTGCTACCCACGCTGCGCAGCCGCTGCCAGCAGTGGTCGTTTTCCCGCCCGTCGTC
GGGGCAACTGGCGCAATGGCAGGGGCACGCCGGTGCCGCAAGCGCGCTGCTGGATGTAGTGGGCGGCATGCCCCTGGCCG
CGGTCCGGTTGCAGGAGGCGGGCGGTGACGCGCTGCTCGCACGCTTCGTGCGCGAGTTGGGCGAGCTGCCGGCGGCCGAC
CCCTTGCACCTTGCCGGCCGCTGGGAAGCGTGGCTGAAGTCCAAGGAGGCGATCGCGGCCGGTTTCGGTCTGCCACAGCT
GATCGACTGGATGCAGCGCTGGGTGGCCGATCTGGCGGCGCTCCGGCTGGGCGGCCGGGTACGTTTCTTCTCCGCCCAGC
AGGCTGCGCTCGCCGCGCTGTGCGAGCGCAGCAGCGTGGCCGCGATAACCAACTGCTACAATGAATTCGCCCAGATCCGC
CGCGTTGCCCAGCATCCATTGAACATGCGGCTGGTGCTCGAAGACATGCTGTTGCGCTATGCGCGCGCCCTTTCCGGAGC
CAGAGGATGA

Upstream 100 bases:

>100_bases
CGCAGGCAGCGCCCCAGCGCATCCGCGTGATCGACGCCAACCGCCCGCCGGAAGTGATCCGCGCCGAGATCGAGCAACTC
GTGGTGGAGCGCTGGTTCGC

Downstream 100 bases:

>100_bases
GCGAACCCGCCGCACCCAGACCGCAGGTGGCGCGCCCCAGCGTGCTGTCGCTGAACATCAATTCAAAGTCGGCGTTGTAT
GCGGCCTACATGCCCTTTCT

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIHPWLQPVWSRLVALEGRLPHALLFIGPAGLGKRDLAEMLAARLLCDSPAADGAACGRCAACAWRLAGNHPDFYHLVPA
ADAEKEAAAEGGDGEGGTTKAKSAQIVIEQIRDLQEALTVTGHHSARRVVVVDPAEAMNVFTANALLKLLEEPPAGCTFV
LVSSAPRRLLPTLRSRCQQWSFSRPSSGQLAQWQGHAGAASALLDVVGGMPLAAVRLQEAGGDALLARFVRELGELPAAD
PLHLAGRWEAWLKSKEAIAAGFGLPQLIDWMQRWVADLAALRLGGRVRFFSAQQAALAALCERSSVAAITNCYNEFAQIR
RVAQHPLNMRLVLEDMLLRYARALSGARG

Sequences:

>Translated_349_residues
MIHPWLQPVWSRLVALEGRLPHALLFIGPAGLGKRDLAEMLAARLLCDSPAADGAACGRCAACAWRLAGNHPDFYHLVPA
ADAEKEAAAEGGDGEGGTTKAKSAQIVIEQIRDLQEALTVTGHHSARRVVVVDPAEAMNVFTANALLKLLEEPPAGCTFV
LVSSAPRRLLPTLRSRCQQWSFSRPSSGQLAQWQGHAGAASALLDVVGGMPLAAVRLQEAGGDALLARFVRELGELPAAD
PLHLAGRWEAWLKSKEAIAAGFGLPQLIDWMQRWVADLAALRLGGRVRFFSAQQAALAALCERSSVAAITNCYNEFAQIR
RVAQHPLNMRLVLEDMLLRYARALSGARG
>Mature_349_residues
MIHPWLQPVWSRLVALEGRLPHALLFIGPAGLGKRDLAEMLAARLLCDSPAADGAACGRCAACAWRLAGNHPDFYHLVPA
ADAEKEAAAEGGDGEGGTTKAKSAQIVIEQIRDLQEALTVTGHHSARRVVVVDPAEAMNVFTANALLKLLEEPPAGCTFV
LVSSAPRRLLPTLRSRCQQWSFSRPSSGQLAQWQGHAGAASALLDVVGGMPLAAVRLQEAGGDALLARFVRELGELPAAD
PLHLAGRWEAWLKSKEAIAAGFGLPQLIDWMQRWVADLAALRLGGRVRFFSAQQAALAALCERSSVAAITNCYNEFAQIR
RVAQHPLNMRLVLEDMLLRYARALSGARG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=193, Percent_Identity=35.7512953367876, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786676, Length=166, Percent_Identity=28.9156626506024, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37490; Mature: 37490

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHPWLQPVWSRLVALEGRLPHALLFIGPAGLGKRDLAEMLAARLLCDSPAADGAACGRC
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
AACAWRLAGNHPDFYHLVPAADAEKEAAAEGGDGEGGTTKAKSAQIVIEQIRDLQEALTV
HHHHHHHCCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGHHSARRVVVVDPAEAMNVFTANALLKLLEEPPAGCTFVLVSSAPRRLLPTLRSRCQQW
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHC
SFSRPSSGQLAQWQGHAGAASALLDVVGGMPLAAVRLQEAGGDALLARFVRELGELPAAD
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
PLHLAGRWEAWLKSKEAIAAGFGLPQLIDWMQRWVADLAALRLGGRVRFFSAQQAALAAL
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHH
CERSSVAAITNCYNEFAQIRRVAQHPLNMRLVLEDMLLRYARALSGARG
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIHPWLQPVWSRLVALEGRLPHALLFIGPAGLGKRDLAEMLAARLLCDSPAADGAACGRC
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
AACAWRLAGNHPDFYHLVPAADAEKEAAAEGGDGEGGTTKAKSAQIVIEQIRDLQEALTV
HHHHHHHCCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGHHSARRVVVVDPAEAMNVFTANALLKLLEEPPAGCTFVLVSSAPRRLLPTLRSRCQQW
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHC
SFSRPSSGQLAQWQGHAGAASALLDVVGGMPLAAVRLQEAGGDALLARFVRELGELPAAD
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
PLHLAGRWEAWLKSKEAIAAGFGLPQLIDWMQRWVADLAALRLGGRVRFFSAQQAALAAL
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHH
CERSSVAAITNCYNEFAQIRRVAQHPLNMRLVLEDMLLRYARALSGARG
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]