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The map label for this gene is cumB [H]

Identifier: 119897879

GI number: 119897879

Start: 1746847

End: 1747311

Strand: Direct

Name: cumB [H]

Synonym: azo1588

Alternate gene names: 119897879

Gene position: 1746847-1747311 (Clockwise)

Preceding gene: 119897874

Following gene: 119897880

Centisome position: 39.92

GC content: 69.89

Gene sequence:

>465_bases
ATGAAGGACGAAATGTTCATGCGTGCGGCGCTTGAACAAGCCCATCTCGCCGGCGCCTGTGACGAGGTGCCGGTCGGCGC
GGTGGTGGTATGCGAGGGCGAGATCGTCGGCCGCGGCTTCAATCAGCCCATCGGCCGCCACGACCCGACCGCCCACGCCG
AGGTGATGGCCTTGCGCGACGCGGCCGCCCGGCTCGGAAACTACCGGCTGCCCGGCTGCGAACTCTATGTGACGCTGGAG
CCCTGCGTCATGTGCTCGGGCGCCATCATGCACGCCCGCATCGCCCGGGTCGTCTACGGCGCGCGCGACCCGAAGACGGG
CGTCGCGGGGAGCGTGATCGACCTCTACGCCGAGGGGCGGCTGAATCACCACGCCAGTATCGAGGGCGGCCTGCTTGCGG
AGGAGTGTGGCGGGCTCCTGTCCAGTTTCTTTGCCGCGCGCCGGCGGCGGACCTCGGTGGCCTGA

Upstream 100 bases:

>100_bases
TCTCTTCTATAGTACTTAGGTAATAGCTAATGGCAAGTGTCGAACGGAAAAGTGATCAGCTCCTTTACACTTCTGTCCTC
AGCAAAGGAGCAATGCCCGA

Downstream 100 bases:

>100_bases
GATGCGGATCCGGATCGACCTTGGCAGCCAGACCCTGGCGCTGTTTGGCGACGACGGCGCCTGCATCCGCCGCTACCCGG
TGTCGACCGCCGCCAACGGC

Product: cytidine deaminase

Products: uridine; NH3

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKDEMFMRAALEQAHLAGACDEVPVGAVVVCEGEIVGRGFNQPIGRHDPTAHAEVMALRDAAARLGNYRLPGCELYVTLE
PCVMCSGAIMHARIARVVYGARDPKTGVAGSVIDLYAEGRLNHHASIEGGLLAEECGGLLSSFFAARRRRTSVA

Sequences:

>Translated_154_residues
MKDEMFMRAALEQAHLAGACDEVPVGAVVVCEGEIVGRGFNQPIGRHDPTAHAEVMALRDAAARLGNYRLPGCELYVTLE
PCVMCSGAIMHARIARVVYGARDPKTGVAGSVIDLYAEGRLNHHASIEGGLLAEECGGLLSSFFAARRRRTSVA
>Mature_154_residues
MKDEMFMRAALEQAHLAGACDEVPVGAVVVCEGEIVGRGFNQPIGRHDPTAHAEVMALRDAAARLGNYRLPGCELYVTLE
PCVMCSGAIMHARIARVVYGARDPKTGVAGSVIDLYAEGRLNHHASIEGGLLAEECGGLLSSFFAARRRRTSVA

Specific function: Deaminates adenosine-34 to inosine in tRNA-Arg2 [H]

COG id: COG0590

COG function: function code FJ; Cytosine/adenosine deaminases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytidine and deoxycytidylate deaminase family [H]

Homologues:

Organism=Homo sapiens, GI124107608, Length=151, Percent_Identity=33.112582781457, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI19923945, Length=68, Percent_Identity=47.0588235294118, Blast_Score=63, Evalue=9e-11,
Organism=Escherichia coli, GI145693172, Length=146, Percent_Identity=56.8493150684932, Blast_Score=171, Evalue=3e-44,
Organism=Escherichia coli, GI1786616, Length=161, Percent_Identity=37.2670807453416, Blast_Score=61, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17541138, Length=152, Percent_Identity=37.5, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17554608, Length=97, Percent_Identity=42.2680412371134, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17555996, Length=79, Percent_Identity=43.0379746835443, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322425, Length=157, Percent_Identity=30.5732484076433, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355527, Length=145, Percent_Identity=34.4827586206897, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR016193 [H]

Pfam domain/function: PF00383 dCMP_cyt_deam_1 [H]

EC number: 3.5.4.5

Molecular weight: Translated: 16422; Mature: 16422

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEMFMRAALEQAHLAGACDEVPVGAVVVCEGEIVGRGFNQPIGRHDPTAHAEVMALRD
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEECCCCCCCCCCCCCCCHHHHHHHHH
AAARLGNYRLPGCELYVTLEPCVMCSGAIMHARIARVVYGARDPKTGVAGSVIDLYAEGR
HHHHHCCCCCCCEEEEEEECHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCC
LNHHASIEGGLLAEECGGLLSSFFAARRRRTSVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKDEMFMRAALEQAHLAGACDEVPVGAVVVCEGEIVGRGFNQPIGRHDPTAHAEVMALRD
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEECCCCCCCCCCCCCCCHHHHHHHHH
AAARLGNYRLPGCELYVTLEPCVMCSGAIMHARIARVVYGARDPKTGVAGSVIDLYAEGR
HHHHHCCCCCCCEEEEEEECHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCC
LNHHASIEGGLLAEECGGLLSSFFAARRRRTSVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytidine; H2O

Specific reaction: cytidine + H2O = uridine + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]