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The map label for this gene is prfB [H]

Identifier: 119897788

GI number: 119897788

Start: 1639694

End: 1640815

Strand: Reverse

Name: prfB [H]

Synonym: azo1497

Alternate gene names: 119897788

Gene position: 1640815-1639694 (Counterclockwise)

Preceding gene: 119897790

Following gene: 119897787

Centisome position: 37.5

GC content: 63.99

Gene sequence:

>1122_bases
TTGTCCCGGAGCCGCACATGGAAGCCGAACGCAAGAACGCCATCGCCGAAAAACTCGCCGACCTCGCCGCCCGCGGTCGC
GAACTCCGGAGGTATCTTTGACTACGATGAGAAATCATCGAAGCTCGAAGAGGTAAACCGCGCACTCGAAGACCCCGCGG
TGTGGAACGACGCCGCGCGCGCGCAGGAACTCGGCAAGGAAAAGCGCCAGCTCGAAGGCGTGGTGCTGACACTGCAGCAG
ATCGAACAGCAGTCGGCGGACCTGAAGGACCTGTTCGACCTCGCCGACATGGAAGGCGACGACGACACCTTCGAGGCGGT
CGAGGGCGACCTCGTGGGCCTGGAAGCTCTGGTCGGCCAGCTCGAATTCCGCCGCATGTTCTCCAACCCGATGGACCCCA
ACGCCTGCTTCATCGAAATCCAGGCCGGCGCCGGCGGCACCGAAGCGCAGGACTGGGCCGGCATGCTGGAGCGCATGTAT
CTGCGCTACGGCGAGAAGAAGGGCTTCACCGTCGAACTGATGGAAGAGACCGAGGGCGAAGTCGCCGGCATCAAGAACTG
CACGATCAAATTCACCGGCGACTACGCCTACGGCTTCCTGCGCACCGAAACCGGCATCCACCGCCTGGTGCGCAAGAGCC
CGTTCGACTCCAACGCCCGCCGCCACACCAGCTTCACCTCGGTGTTCGTGTATCCGGAGGTCGATGACTCGATCGAGATC
GAGATCAACCCTGCCGACCTGCGCATCGACACCTACCGCGCCTCCGGCGCCGGCGGCCAGCACATCAACAAGACCGATTC
CGCGGTGCGCATCACCCACCAGCCGACCGGCATCGTCGTGCAGTGCCAGAACGACCGTTCGCAGCACCGCAACAAGGACG
AGGCGATGTCGATGCTGAAGGCGCGCCTGTACGAACTGGAACTGCGCAAGCGCCAGGCCGAGCAGCAGAAGCTGGAAGAC
GCCAAGAGCGACATCGGCTGGGGCCACCAGATCCGCAGCTACGTGCTCGACCAGTCGCGCATCAAGGACCTGCGCACCGG
CTACGAAGTCGGCAACACCCAGGCGGTGCTCGACGGCGACCTCGAAGATTTCATCGCCGCCAGCCTCAAGCAGGGCGTCT
GA

Upstream 100 bases:

>100_bases
GTGATGTCCATCTCGCGGGCACGCAGGGGGAAAGGCGCGCAGTTTAGCCTGCGGCGCCCGGGCGGGCCGCCCTCCCCGGT
ATAATGCCGGGTTTTCCGCA

Downstream 100 bases:

>100_bases
GCCCCCCGCGTTCCACCCGATGAGCCGGCCCGCCGCGGCCGGCTCCGTTTTTTGATGACCGCTCCAGACCTCTCGGACTT
TCTCGCCGCCGGCGTCGTCC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSRSRTWKPNARTPSPKNSPTSPPAVANSGGIFDYDEKSSKLEEVNRALEDPAVWNDAARAQELGKEKRQLEGVVLTLQQ
IEQQSADLKDLFDLADMEGDDDTFEAVEGDLVGLEALVGQLEFRRMFSNPMDPNACFIEIQAGAGGTEAQDWAGMLERMY
LRYGEKKGFTVELMEETEGEVAGIKNCTIKFTGDYAYGFLRTETGIHRLVRKSPFDSNARRHTSFTSVFVYPEVDDSIEI
EINPADLRIDTYRASGAGGQHINKTDSAVRITHQPTGIVVQCQNDRSQHRNKDEAMSMLKARLYELELRKRQAEQQKLED
AKSDIGWGHQIRSYVLDQSRIKDLRTGYEVGNTQAVLDGDLEDFIAASLKQGV

Sequences:

>Translated_373_residues
MSRSRTWKPNARTPSPKNSPTSPPAVANSGGIFDYDEKSSKLEEVNRALEDPAVWNDAARAQELGKEKRQLEGVVLTLQQ
IEQQSADLKDLFDLADMEGDDDTFEAVEGDLVGLEALVGQLEFRRMFSNPMDPNACFIEIQAGAGGTEAQDWAGMLERMY
LRYGEKKGFTVELMEETEGEVAGIKNCTIKFTGDYAYGFLRTETGIHRLVRKSPFDSNARRHTSFTSVFVYPEVDDSIEI
EINPADLRIDTYRASGAGGQHINKTDSAVRITHQPTGIVVQCQNDRSQHRNKDEAMSMLKARLYELELRKRQAEQQKLED
AKSDIGWGHQIRSYVLDQSRIKDLRTGYEVGNTQAVLDGDLEDFIAASLKQGV
>Mature_372_residues
SRSRTWKPNARTPSPKNSPTSPPAVANSGGIFDYDEKSSKLEEVNRALEDPAVWNDAARAQELGKEKRQLEGVVLTLQQI
EQQSADLKDLFDLADMEGDDDTFEAVEGDLVGLEALVGQLEFRRMFSNPMDPNACFIEIQAGAGGTEAQDWAGMLERMYL
RYGEKKGFTVELMEETEGEVAGIKNCTIKFTGDYAYGFLRTETGIHRLVRKSPFDSNARRHTSFTSVFVYPEVDDSIEIE
INPADLRIDTYRASGAGGQHINKTDSAVRITHQPTGIVVQCQNDRSQHRNKDEAMSMLKARLYELELRKRQAEQQKLEDA
KSDIGWGHQIRSYVLDQSRIKDLRTGYEVGNTQAVLDGDLEDFIAASLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=281, Percent_Identity=35.9430604982206, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI34577120, Length=236, Percent_Identity=39.8305084745763, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI166795305, Length=181, Percent_Identity=36.4640883977901, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=67.3469387755102, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1787462, Length=331, Percent_Identity=39.8791540785499, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=242, Percent_Identity=30.1652892561983, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=249, Percent_Identity=35.3413654618474, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19921226, Length=303, Percent_Identity=36.3036303630363, Blast_Score=158, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41793; Mature: 41662

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSRTWKPNARTPSPKNSPTSPPAVANSGGIFDYDEKSSKLEEVNRALEDPAVWNDAAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
AQELGKEKRQLEGVVLTLQQIEQQSADLKDLFDLADMEGDDDTFEAVEGDLVGLEALVGQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHH
LEFRRMFSNPMDPNACFIEIQAGAGGTEAQDWAGMLERMYLRYGEKKGFTVELMEETEGE
HHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VAGIKNCTIKFTGDYAYGFLRTETGIHRLVRKSPFDSNARRHTSFTSVFVYPEVDDSIEI
EECCCCCEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEE
EINPADLRIDTYRASGAGGQHINKTDSAVRITHQPTGIVVQCQNDRSQHRNKDEAMSMLK
EEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHH
ARLYELELRKRQAEQQKLEDAKSDIGWGHQIRSYVLDQSRIKDLRTGYEVGNTQAVLDGD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
LEDFIAASLKQGV
HHHHHHHHHHHCC
>Mature Secondary Structure 
SRSRTWKPNARTPSPKNSPTSPPAVANSGGIFDYDEKSSKLEEVNRALEDPAVWNDAAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
AQELGKEKRQLEGVVLTLQQIEQQSADLKDLFDLADMEGDDDTFEAVEGDLVGLEALVGQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHH
LEFRRMFSNPMDPNACFIEIQAGAGGTEAQDWAGMLERMYLRYGEKKGFTVELMEETEGE
HHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VAGIKNCTIKFTGDYAYGFLRTETGIHRLVRKSPFDSNARRHTSFTSVFVYPEVDDSIEI
EECCCCCEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEE
EINPADLRIDTYRASGAGGQHINKTDSAVRITHQPTGIVVQCQNDRSQHRNKDEAMSMLK
EEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHH
ARLYELELRKRQAEQQKLEDAKSDIGWGHQIRSYVLDQSRIKDLRTGYEVGNTQAVLDGD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
LEDFIAASLKQGV
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA