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The map label for this gene is kdsB

Identifier: 119897763

GI number: 119897763

Start: 1603733

End: 1604506

Strand: Direct

Name: kdsB

Synonym: azo1472

Alternate gene names: 119897763

Gene position: 1603733-1604506 (Clockwise)

Preceding gene: 119897762

Following gene: 119897764

Centisome position: 36.65

GC content: 72.09

Gene sequence:

>774_bases
ATGTCCGCCTTCCGCATCGTCATTCCCGCCCGTTTCGCCTCCAGCCGGCTGCCGGGCAAGCCGCTCGCCGACATTGCCGG
CAAGCCGATGATCGTCCGCGTGCTCGAACGCGTGCAGGACGCCGGCGCCGCAGAGGTGTGGGTGGCCACCGACCACGATG
GCGTGCGCGAGGCGGTTGAGGCGGCCGGCGGCAAGGTGGTCATGACCCGCGCCGACCATCCCAGCGGCACCGATCGCCTC
GCCGAGGTCGCGTGCGCGCTCGGCTGGCAGGACGAGGACATCGTCGTCAACGTGCAGGGCGACGAGCCGCTGATCGACCC
CGCCATCATCACCGCGGTGGCGGCCGAACTCGCGGCCGACGGCGAAGCCGCCATCGCCACCGCGGCGCATCCGATCCACG
ACGCCGACGAAGTCTTCAACCCCAACGTCGTCAAGGTGGTGTGCGACGCCCGGCAGCGCGCGCTGTATTTCTCCCGCGCG
CCGATTCCCTGGGCCCGCGATGCCTGGGCGCAACCCGGCGAGCGCGCCGTCCTGCCGGCCGGACTTCCGGTGCTGCGCCA
TGTCGGGCTGTATGCCTACCGAGTCAGCTTCCTGCGCCGCTACACCGCGCTGGCGCCCGCGCCGATCGAGCAGTGGGAAG
CGCTGGAGCAGCTGCGGGCGATGTGGCACGGCTACCGCATCCGCGTGCTCGGGCTGCAGGCCGCGCCGGCCGCGGGGGTC
GATACCGTGGAAGACCTGGAGCGGGTGCGGGCGGTATTCGCCGGCGCTGCTTGA

Upstream 100 bases:

>100_bases
GCCTCGCGTTCCCGATCCGCGACGGCATCCCGGTGATGCTGGAAGAGGAAGCGCGCGCGTTGGCCGCCGAGGACGTGGAC
GCGCTGCGTTGAGCGCCGCC

Downstream 100 bases:

>100_bases
CACGTTTTGCGCGGTCTTTCGGTTTGACCGTGCCGGTCGAGTCGTTTAAGTTTCGGCCTTTTGGTTCATCTAGTTCATAA
AGCAGGCCGGCAGCACAGGC

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSAFRIVIPARFASSRLPGKPLADIAGKPMIVRVLERVQDAGAAEVWVATDHDGVREAVEAAGGKVVMTRADHPSGTDRL
AEVACALGWQDEDIVVNVQGDEPLIDPAIITAVAAELAADGEAAIATAAHPIHDADEVFNPNVVKVVCDARQRALYFSRA
PIPWARDAWAQPGERAVLPAGLPVLRHVGLYAYRVSFLRRYTALAPAPIEQWEALEQLRAMWHGYRIRVLGLQAAPAAGV
DTVEDLERVRAVFAGAA

Sequences:

>Translated_257_residues
MSAFRIVIPARFASSRLPGKPLADIAGKPMIVRVLERVQDAGAAEVWVATDHDGVREAVEAAGGKVVMTRADHPSGTDRL
AEVACALGWQDEDIVVNVQGDEPLIDPAIITAVAAELAADGEAAIATAAHPIHDADEVFNPNVVKVVCDARQRALYFSRA
PIPWARDAWAQPGERAVLPAGLPVLRHVGLYAYRVSFLRRYTALAPAPIEQWEALEQLRAMWHGYRIRVLGLQAAPAAGV
DTVEDLERVRAVFAGAA
>Mature_256_residues
SAFRIVIPARFASSRLPGKPLADIAGKPMIVRVLERVQDAGAAEVWVATDHDGVREAVEAAGGKVVMTRADHPSGTDRLA
EVACALGWQDEDIVVNVQGDEPLIDPAIITAVAAELAADGEAAIATAAHPIHDADEVFNPNVVKVVCDARQRALYFSRAP
IPWARDAWAQPGERAVLPAGLPVLRHVGLYAYRVSFLRRYTALAPAPIEQWEALEQLRAMWHGYRIRVLGLQAAPAAGVD
TVEDLERVRAVFAGAA

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=249, Percent_Identity=57.429718875502, Blast_Score=274, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_AZOSB (A1K5I4)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932976.1
- ProteinModelPortal:   A1K5I4
- SMR:   A1K5I4
- STRING:   A1K5I4
- GeneID:   4610152
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1472
- eggNOG:   COG1212
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- PhylomeDB:   A1K5I4
- ProtClustDB:   PRK05450
- BioCyc:   ASP62928:AZO1472-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27628; Mature: 27497

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFRIVIPARFASSRLPGKPLADIAGKPMIVRVLERVQDAGAAEVWVATDHDGVREAVE
CCCEEEEEECHHHCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
AAGGKVVMTRADHPSGTDRLAEVACALGWQDEDIVVNVQGDEPLIDPAIITAVAAELAAD
HCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
GEAAIATAAHPIHDADEVFNPNVVKVVCDARQRALYFSRAPIPWARDAWAQPGERAVLPA
CCHHEEECCCCCCCHHHHCCCCEEEEEEHHHHHHEECCCCCCCCCCHHHCCCCCCEECCC
GLPVLRHVGLYAYRVSFLRRYTALAPAPIEQWEALEQLRAMWHGYRIRVLGLQAAPAAGV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
DTVEDLERVRAVFAGAA
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAFRIVIPARFASSRLPGKPLADIAGKPMIVRVLERVQDAGAAEVWVATDHDGVREAVE
CCEEEEEECHHHCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
AAGGKVVMTRADHPSGTDRLAEVACALGWQDEDIVVNVQGDEPLIDPAIITAVAAELAAD
HCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
GEAAIATAAHPIHDADEVFNPNVVKVVCDARQRALYFSRAPIPWARDAWAQPGERAVLPA
CCHHEEECCCCCCCHHHHCCCCEEEEEEHHHHHHEECCCCCCCCCCHHHCCCCCCEECCC
GLPVLRHVGLYAYRVSFLRRYTALAPAPIEQWEALEQLRAMWHGYRIRVLGLQAAPAAGV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
DTVEDLERVRAVFAGAA
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA