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The map label for this gene is rnfB2

Identifier: 119897731

GI number: 119897731

Start: 1570179

End: 1570730

Strand: Reverse

Name: rnfB2

Synonym: azo1440

Alternate gene names: 119897731

Gene position: 1570730-1570179 (Counterclockwise)

Preceding gene: 119897732

Following gene: 119897730

Centisome position: 35.89

GC content: 67.21

Gene sequence:

>552_bases
ATGCTGTCTGCCCTTCTCGTCATGGCCGCCATCGCGGTAGTGCTCGGCGCGGCGCTCGGCTTTGCCGCGATCCGCTTCCG
CGTCGAAGGCGACCCGCTGGTCGACAAGATCGACGCGATCCTGCCGCAAACCCAGTGCGGCCAGTGCGGCTACCCCGGCT
GCAAGCCCTACGCGCAGGCCATCGCCCAGGGCGAGGCCGACATCAACCAATGCCCGCCGGGCGGCGAGGAAGGCGTGCGC
AAGCTCGCGGATCTGCTGGGCCGCGAGTTCAAGCCGCTGTCGGCCGAGCATGGCGAGGAAAAGCCGAAGGCGGTGGCCTA
CATCGACGAAAACGTCTGCATCGGCTGCACGCTGTGCCTGCAGGCCTGCCCGGTGGACGCCATCGTCGGCGCCGCCAAGC
AGATGCACACCGTGGTCGATCCGCTGTGCACCGGTTGCGAACTCTGCGTCGCGCCCTGCCCGGTCGACTGTATCTACATG
GAGCCGGTACGCGAAACCGTGCAGACCTGGCGCTGGAAATACCCGGTCGTCGAGATCAGGAAGGCAGCATGA

Upstream 100 bases:

>100_bases
AGCCCTTCCGCGGCACCGCGATCGCGATGATCACCGCCGGCTTCATGAGCCTCGCTTTCATGGGCTTTGCCGGCCTCGAC
CGTTTCCACTGAGCCCCCAG

Downstream 100 bases:

>100_bases
TCGGACGCCTGTTCAGTTTTCGCGGCGGCATCAAGCCCGCCGCCCACAAGGAAGAATCCGCCGGCGCCGCCATCGTCGCC
GCGCCGCTGCCGGCCCAGCT

Product: electron transport complex protein RnfB

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MLSALLVMAAIAVVLGAALGFAAIRFRVEGDPLVDKIDAILPQTQCGQCGYPGCKPYAQAIAQGEADINQCPPGGEEGVR
KLADLLGREFKPLSAEHGEEKPKAVAYIDENVCIGCTLCLQACPVDAIVGAAKQMHTVVDPLCTGCELCVAPCPVDCIYM
EPVRETVQTWRWKYPVVEIRKAA

Sequences:

>Translated_183_residues
MLSALLVMAAIAVVLGAALGFAAIRFRVEGDPLVDKIDAILPQTQCGQCGYPGCKPYAQAIAQGEADINQCPPGGEEGVR
KLADLLGREFKPLSAEHGEEKPKAVAYIDENVCIGCTLCLQACPVDAIVGAAKQMHTVVDPLCTGCELCVAPCPVDCIYM
EPVRETVQTWRWKYPVVEIRKAA
>Mature_183_residues
MLSALLVMAAIAVVLGAALGFAAIRFRVEGDPLVDKIDAILPQTQCGQCGYPGCKPYAQAIAQGEADINQCPPGGEEGVR
KLADLLGREFKPLSAEHGEEKPKAVAYIDENVCIGCTLCLQACPVDAIVGAAKQMHTVVDPLCTGCELCVAPCPVDCIYM
EPVRETVQTWRWKYPVVEIRKAA

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1787915, Length=178, Percent_Identity=55.6179775280899, Blast_Score=205, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFB_AZOSB (A1K5F2)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932944.1
- ProteinModelPortal:   A1K5F2
- SMR:   A1K5F2
- STRING:   A1K5F2
- GeneID:   4609745
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1440
- eggNOG:   COG2878
- HOGENOM:   HBG549899
- OMA:   KFKVEGD
- PhylomeDB:   A1K5F2
- ProtClustDB:   PRK05113
- BioCyc:   ASP62928:AZO1440-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00463
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285
- Gene3D:   G3DSA:1.10.1060.10
- PIRSF:   PIRSF005784
- TIGRFAMs:   TIGR01944

Pfam domain/function: PF00037 Fer4; PF04060 FeS

EC number: NA

Molecular weight: Translated: 19559; Mature: 19559

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
9.3 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
9.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSALLVMAAIAVVLGAALGFAAIRFRVEGDPLVDKIDAILPQTQCGQCGYPGCKPYAQA
CHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
IAQGEADINQCPPGGEEGVRKLADLLGREFKPLSAEHGEEKPKAVAYIDENVCIGCTLCL
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHH
QACPVDAIVGAAKQMHTVVDPLCTGCELCVAPCPVDCIYMEPVRETVQTWRWKYPVVEIR
HHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEHHHHHHHHHHHCCCCCHHHHC
KAA
CCC
>Mature Secondary Structure
MLSALLVMAAIAVVLGAALGFAAIRFRVEGDPLVDKIDAILPQTQCGQCGYPGCKPYAQA
CHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
IAQGEADINQCPPGGEEGVRKLADLLGREFKPLSAEHGEEKPKAVAYIDENVCIGCTLCL
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHH
QACPVDAIVGAAKQMHTVVDPLCTGCELCVAPCPVDCIYMEPVRETVQTWRWKYPVVEIR
HHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEHHHHHHHHHHHCCCCCHHHHC
KAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA