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The map label for this gene is nuoF [H]

Identifier: 119897692

GI number: 119897692

Start: 1528259

End: 1529578

Strand: Direct

Name: nuoF [H]

Synonym: azo1401

Alternate gene names: 119897692

Gene position: 1528259-1529578 (Clockwise)

Preceding gene: 119897691

Following gene: 119897693

Centisome position: 34.92

GC content: 61.82

Gene sequence:

>1320_bases
ATGAGCGCGCACGGACTGATCCTCGCCGGGCTGGACGGCGACCGCACCTGGCGGCTCCAGGACTACATCGGCCGTGGCGG
CTATTCGGCGCTGAAGAAGATCATCGCGGAGAAGATTCCGCCGGAAACGATCATCGCGGAACTCAAGGCTTCCGCCCTGC
GCGGCCGCGGCGGTGCAGGCTTCCCGACGGGGCTGAAGTGGAGCTTCATGCCGCGCTCCTTCCCGGGCGACAAGTATCTC
GCCTGTAATTCGGACGAGGGCGAGCCGGGGACTTTCAAAGACCGCGACATCCTGCGCTACAACCCCCACACGGTGATCGA
AGGCATGGTCATCGCCGCCTATGCGATGGGGGCCACGCGCGGTTACAACTACATCCACGGCGAAGTCTTCGAACTGTACC
AGCGGTTCGAGGAAGCGCTCGACGAAGCGCGTCAGGCCGGGCTGGTCGGCCAGAACATCCTCGGTAGCGAGTTCTCGTTC
GAACTGTTTGCGCACCACGGCTACGGTGCCTACATCTGTGGCGAAGAAACCGCGCTGCTGGAGTCGATCGAAGGCAAGAA
AGGGCAGCCGCGCTTCAAGCCGCCGTTCCCTGCGAGCTATGGCCTCTACGGCAAGCCGACGACGATCAACAACACCGAAA
CGTTCGCCTCGGTTCCCTTCATCATCAACCTGGGCGGTGAAGGCTTCCTGAATCTGGGCAAGCCCAATAACGGCGGTACC
AAGCTGTTTTCGATCTCGGGCCACGTCAATCGTCCTGGCGTCTACGAGATCAACCTCGGCACGCCGTTCTCCGAGCTGCT
CGAAATGGCGGGCGGCATGCGTGGCGGCCGCAAGCTGAAGGCGGTGATTCCCGGCGGTTCGTCGGCGCCGGTGCTGCCGG
GCGACATCATGATGGACTGCACGATGGACTATGACTCCATCTCCAAGGCCGGCTCGATGCTCGGTTCCGGCGCGGTCATC
GTGATGGACGAAACCGTGTGCATGGTCAAGGCCCTCGAGCGCCTGTCCTACTTCTATTTCGAAGAGTCCTGCGGCCAATG
CACGCCGTGCCGCGAGGGTACCGGTTGGTTGTACCGCGTCGTTCATCGCATCGAGAACGGCCTTGGCCGTCCGGACGACC
TCGATCTGCTCAATTCGGTGACCGGCAACATCATGGGCCGCACGATCTGTGCCCTGGGCGACGCCGCGTCGATGCCGGTA
CAGAGTTTCATCAAGCATTTCGGTGCTGAGTTCGAATACCACATCGAAAACAAGAAATGCCTGGTTTCGCCCGAAGTCCA
GTACGCGGGTAGCCAAATCTACGTGAGCCCCTCATGCTAG

Upstream 100 bases:

>100_bases
CCTGTGGTGACGCCCCGGTTCTGCTGCACAACAACCACACGATGTGCAGCTGGATGACGACTGAGAAAATCGACCAACTC
CTGGCCGACCTGGGAAGCAA

Downstream 100 bases:

>100_bases
AGATCGAAATCGACGGTAAGCAGCTGCAGGTCGCTGACGGCAGCACGGTAATGGACGCCGCCGCCCGCGCCGGCGTCTAT
ATCCCGCACTTCTGCTACCA

Product: putative NADH-ubiquinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 1; NDH-1 subunit F 1 [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSAHGLILAGLDGDRTWRLQDYIGRGGYSALKKIIAEKIPPETIIAELKASALRGRGGAGFPTGLKWSFMPRSFPGDKYL
ACNSDEGEPGTFKDRDILRYNPHTVIEGMVIAAYAMGATRGYNYIHGEVFELYQRFEEALDEARQAGLVGQNILGSEFSF
ELFAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASYGLYGKPTTINNTETFASVPFIINLGGEGFLNLGKPNNGGT
KLFSISGHVNRPGVYEINLGTPFSELLEMAGGMRGGRKLKAVIPGGSSAPVLPGDIMMDCTMDYDSISKAGSMLGSGAVI
VMDETVCMVKALERLSYFYFEESCGQCTPCREGTGWLYRVVHRIENGLGRPDDLDLLNSVTGNIMGRTICALGDAASMPV
QSFIKHFGAEFEYHIENKKCLVSPEVQYAGSQIYVSPSC

Sequences:

>Translated_439_residues
MSAHGLILAGLDGDRTWRLQDYIGRGGYSALKKIIAEKIPPETIIAELKASALRGRGGAGFPTGLKWSFMPRSFPGDKYL
ACNSDEGEPGTFKDRDILRYNPHTVIEGMVIAAYAMGATRGYNYIHGEVFELYQRFEEALDEARQAGLVGQNILGSEFSF
ELFAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASYGLYGKPTTINNTETFASVPFIINLGGEGFLNLGKPNNGGT
KLFSISGHVNRPGVYEINLGTPFSELLEMAGGMRGGRKLKAVIPGGSSAPVLPGDIMMDCTMDYDSISKAGSMLGSGAVI
VMDETVCMVKALERLSYFYFEESCGQCTPCREGTGWLYRVVHRIENGLGRPDDLDLLNSVTGNIMGRTICALGDAASMPV
QSFIKHFGAEFEYHIENKKCLVSPEVQYAGSQIYVSPSC
>Mature_438_residues
SAHGLILAGLDGDRTWRLQDYIGRGGYSALKKIIAEKIPPETIIAELKASALRGRGGAGFPTGLKWSFMPRSFPGDKYLA
CNSDEGEPGTFKDRDILRYNPHTVIEGMVIAAYAMGATRGYNYIHGEVFELYQRFEEALDEARQAGLVGQNILGSEFSFE
LFAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASYGLYGKPTTINNTETFASVPFIINLGGEGFLNLGKPNNGGTK
LFSISGHVNRPGVYEINLGTPFSELLEMAGGMRGGRKLKAVIPGGSSAPVLPGDIMMDCTMDYDSISKAGSMLGSGAVIV
MDETVCMVKALERLSYFYFEESCGQCTPCREGTGWLYRVVHRIENGLGRPDDLDLLNSVTGNIMGRTICALGDAASMPVQ
SFIKHFGAEFEYHIENKKCLVSPEVQYAGSQIYVSPSC

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=414, Percent_Identity=50.4830917874396, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI260656005, Length=414, Percent_Identity=50.4830917874396, Blast_Score=410, Evalue=1e-114,
Organism=Escherichia coli, GI1788620, Length=414, Percent_Identity=44.6859903381643, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17531727, Length=412, Percent_Identity=49.2718446601942, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI19920794, Length=415, Percent_Identity=49.6385542168675, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45550452, Length=415, Percent_Identity=47.4698795180723, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24653819, Length=414, Percent_Identity=36.4734299516908, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24653817, Length=411, Percent_Identity=33.5766423357664, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47699; Mature: 47568

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHGLILAGLDGDRTWRLQDYIGRGGYSALKKIIAEKIPPETIIAELKASALRGRGGAG
CCCCCEEEEECCCCCEEEHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
FPTGLKWSFMPRSFPGDKYLACNSDEGEPGTFKDRDILRYNPHTVIEGMVIAAYAMGATR
CCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHC
GYNYIHGEVFELYQRFEEALDEARQAGLVGQNILGSEFSFELFAHHGYGAYICGEETALL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEECCHHHHH
ESIEGKKGQPRFKPPFPASYGLYGKPTTINNTETFASVPFIINLGGEGFLNLGKPNNGGT
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEECCEEEEECCCCEEECCCCCCCCE
KLFSISGHVNRPGVYEINLGTPFSELLEMAGGMRGGRKLKAVIPGGSSAPVLPGDIMMDC
EEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEE
TMDYDSISKAGSMLGSGAVIVMDETVCMVKALERLSYFYFEESCGQCTPCREGTGWLYRV
ECCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
VHRIENGLGRPDDLDLLNSVTGNIMGRTICALGDAASMPVQSFIKHFGAEFEYHIENKKC
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCEE
LVSPEVQYAGSQIYVSPSC
EECCCHHHCCCEEEECCCC
>Mature Secondary Structure 
SAHGLILAGLDGDRTWRLQDYIGRGGYSALKKIIAEKIPPETIIAELKASALRGRGGAG
CCCCEEEEECCCCCEEEHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
FPTGLKWSFMPRSFPGDKYLACNSDEGEPGTFKDRDILRYNPHTVIEGMVIAAYAMGATR
CCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHC
GYNYIHGEVFELYQRFEEALDEARQAGLVGQNILGSEFSFELFAHHGYGAYICGEETALL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEECCHHHHH
ESIEGKKGQPRFKPPFPASYGLYGKPTTINNTETFASVPFIINLGGEGFLNLGKPNNGGT
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEECCEEEEECCCCEEECCCCCCCCE
KLFSISGHVNRPGVYEINLGTPFSELLEMAGGMRGGRKLKAVIPGGSSAPVLPGDIMMDC
EEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEE
TMDYDSISKAGSMLGSGAVIVMDETVCMVKALERLSYFYFEESCGQCTPCREGTGWLYRV
ECCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
VHRIENGLGRPDDLDLLNSVTGNIMGRTICALGDAASMPVQSFIKHFGAEFEYHIENKKC
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCEE
LVSPEVQYAGSQIYVSPSC
EECCCHHHCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]