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The map label for this gene is tpiA

Identifier: 119897685

GI number: 119897685

Start: 1523592

End: 1524320

Strand: Direct

Name: tpiA

Synonym: azo1394

Alternate gene names: 119897685

Gene position: 1523592-1524320 (Clockwise)

Preceding gene: 119897684

Following gene: 119897686

Centisome position: 34.82

GC content: 65.71

Gene sequence:

>729_bases
GTGACCACGAAACTGATAGCAGGCAACTGGAAGCTCAACGGCAGCCTGGCGAAGAACGCAGCGCTGATCGACGAGTTGCG
GCGCGCCGAGATGCACTGCGTCGTTTGTGTGCCGTATCCGTATCTGGCCCAGGCGCAGGCGCTTGTTGCCGGGTCGTTGA
TCGAACTCGGTGCCCAGGACGTCAGCGAGTATGAGCAGGGCGCCTATACCGGCGAAGTTTCCGCCGCGATGCTCGTCGAG
TTCGGCTGCCGGTATGTCATCGTCGGTCACTCCGAACGCCGCGCGTTGTTTGGAGATTCCGACCAGGTCGTCGGCCGCAA
GGCGGCCTCTGCGCTTGCCGCCGGGCTCACGCCCATCGTCTGCGTGGGTGAAACGCTGGCGGAGCGCGAGCTTGGGGAAG
TCGAAGCGGTCATCCGCCGTCAACTGCAAGCCGTTGCCGATTGCGTAGGGGGCGAGGCGCTGCCGACGTTGGTGGTCGCT
TACGAGCCGGTGTGGGCCATTGGCACCGGCCGCTCGGCTACGCCGGAGCAGGTCGCGCAGACGCACGGCTTCATCCGCGC
ATGGTTTTCGGCCCGCTGCGACGCTTCGGCTGTGCGCATCCTTTATGGCGGCAGTGTCAAGCCCGAAAATGCGGCGGTGC
TGTTCTCCACGGACGACGTCGATGGCGGCCTGATCGGCGGGGCTTCGCTGGTCGGTAGCGACTTCGTTGCCATCTGCCGC
GCCGCCTGA

Upstream 100 bases:

>100_bases
GGCAGCCCGGCAGGGTCGGTACGGCCTTGGCCGGGCTGCCGCCGTTTGCCTGCTCCCCAACGCAAGCCTATAATCTCGCG
ATTTACCGGGCAGCCAACGC

Downstream 100 bases:

>100_bases
CGGGCGGTTTTTCAAACCAAAACGCTACTTTCAAGGGTTGTGATGAGCGACATTCTGTTTTCGGTGGTCCTGACGGTGCA
CGTCGTCGTAGGGCTCGGTG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTTKLIAGNWKLNGSLAKNAALIDELRRAEMHCVVCVPYPYLAQAQALVAGSLIELGAQDVSEYEQGAYTGEVSAAMLVE
FGCRYVIVGHSERRALFGDSDQVVGRKAASALAAGLTPIVCVGETLAERELGEVEAVIRRQLQAVADCVGGEALPTLVVA
YEPVWAIGTGRSATPEQVAQTHGFIRAWFSARCDASAVRILYGGSVKPENAAVLFSTDDVDGGLIGGASLVGSDFVAICR
AA

Sequences:

>Translated_242_residues
MTTKLIAGNWKLNGSLAKNAALIDELRRAEMHCVVCVPYPYLAQAQALVAGSLIELGAQDVSEYEQGAYTGEVSAAMLVE
FGCRYVIVGHSERRALFGDSDQVVGRKAASALAAGLTPIVCVGETLAERELGEVEAVIRRQLQAVADCVGGEALPTLVVA
YEPVWAIGTGRSATPEQVAQTHGFIRAWFSARCDASAVRILYGGSVKPENAAVLFSTDDVDGGLIGGASLVGSDFVAICR
AA
>Mature_241_residues
TTKLIAGNWKLNGSLAKNAALIDELRRAEMHCVVCVPYPYLAQAQALVAGSLIELGAQDVSEYEQGAYTGEVSAAMLVEF
GCRYVIVGHSERRALFGDSDQVVGRKAASALAAGLTPIVCVGETLAERELGEVEAVIRRQLQAVADCVGGEALPTLVVAY
EPVWAIGTGRSATPEQVAQTHGFIRAWFSARCDASAVRILYGGSVKPENAAVLFSTDDVDGGLIGGASLVGSDFVAICRA
A

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=243, Percent_Identity=41.9753086419753, Blast_Score=177, Evalue=7e-45,
Organism=Homo sapiens, GI226529917, Length=243, Percent_Identity=41.9753086419753, Blast_Score=177, Evalue=8e-45,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=47.4103585657371, Blast_Score=207, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17536593, Length=250, Percent_Identity=45.2, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=40.650406504065, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI28572008, Length=249, Percent_Identity=44.1767068273092, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28572006, Length=249, Percent_Identity=44.1767068273092, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28572004, Length=249, Percent_Identity=44.1767068273092, Blast_Score=188, Evalue=2e-48,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_AZOSB (A1K5A6)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932898.1
- ProteinModelPortal:   A1K5A6
- SMR:   A1K5A6
- STRING:   A1K5A6
- GeneID:   4609641
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1394
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   A1K5A6
- ProtClustDB:   PRK00042
- BioCyc:   ASP62928:AZO1394-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 25360; Mature: 25229

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 90-90 ACT_SITE 162-162 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKLIAGNWKLNGSLAKNAALIDELRRAEMHCVVCVPYPYLAQAQALVAGSLIELGAQD
CCCEEEECCEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHCHHH
VSEYEQGAYTGEVSAAMLVEFGCRYVIVGHSERRALFGDSDQVVGRKAASALAAGLTPIV
HHHHHCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
CVGETLAERELGEVEAVIRRQLQAVADCVGGEALPTLVVAYEPVWAIGTGRSATPEQVAQ
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCEEEECCCCCCCHHHHHH
THGFIRAWFSARCDASAVRILYGGSVKPENAAVLFSTDDVDGGLIGGASLVGSDFVAICR
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEECCHHHHHHHHHHHHC
AA
CC
>Mature Secondary Structure 
TTKLIAGNWKLNGSLAKNAALIDELRRAEMHCVVCVPYPYLAQAQALVAGSLIELGAQD
CCEEEECCEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHCHHH
VSEYEQGAYTGEVSAAMLVEFGCRYVIVGHSERRALFGDSDQVVGRKAASALAAGLTPIV
HHHHHCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
CVGETLAERELGEVEAVIRRQLQAVADCVGGEALPTLVVAYEPVWAIGTGRSATPEQVAQ
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCEEEECCCCCCCHHHHHH
THGFIRAWFSARCDASAVRILYGGSVKPENAAVLFSTDDVDGGLIGGASLVGSDFVAICR
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEECCHHHHHHHHHHHHC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA