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The map label for this gene is narK [H]

Identifier: 119897579

GI number: 119897579

Start: 1397768

End: 1398982

Strand: Direct

Name: narK [H]

Synonym: azo1288

Alternate gene names: 119897579

Gene position: 1397768-1398982 (Clockwise)

Preceding gene: 119897574

Following gene: 119897580

Centisome position: 31.94

GC content: 60.0

Gene sequence:

>1215_bases
ATGGATAAGAAATCGTTTCTGCAGGCAGGTCATACTCCGACCCTGTTTGCCGCGTTCATGTATTTCGATCTCGCCTTCAT
GGTGTGGGTCATGTTAGGGCCGCTTGGAGTACAGATCGCGACCGATCTCGGCTTAACCCACGCCCAGAAAGGAATGATGG
TGGCGACACCGGTTCTCGCCGGGGCGTTGTTGCGTTTCGTCATGGGCATGCTGGTAGATCATCTGAAGCCAAAGCTGACC
GGTGCAATCGGGCAGGTGATCGTCATCGCTGCCTTGGCTTATGCGTGGCAGCATGGCATCCACTCCTTTGAAGAAACCTT
GATTCTCGGCCTCTTCCTTGGCGTTGCGGGCGCCTCGTTTGCCGTGGCGTTGCCGCTCGCGTCGCGCTGGTACCCGCCGG
AGCACCAAGGGACGGCCTTGGGTATCGCGGGTGCGGGCAACTCGGGAACCGCGCTGGCGGCGCTGTTTGGCCCGGGCCTC
GCGGCGAGCTTCGGCTGGGTGAATGTCTTCGGGCTGGCGTTGATCCCGCTTGGCTTGGTCTTTGTTGCATATCTGCTGAT
GGCGAAGGATGCGCCGGAGTGTCCTCCGCCGAAGCGACTCGGTGAGTATCTGGCTGTCCTGAAAGATAAGGATGCCTGGT
GGTTCATGTTCTTCTACAGCGTGACGTTCGGCGGCTTCGTCGGTCTGGCCTCGTCGCTGACGATCTATTTCAATACCCAG
TATGGCCTTGACGCGAAGATGGCCGGGTACTTCACCGCCGCGTGTGTTTTTGCTGGCAGCCTGGTACGTCCGATCGGGGG
CAATCTGGCCGACAGGATAGGGGGCATCAAGTCGCTTTCCGTGGTGTATATCGCTGCGGCGGTCGCCTTGAGCATCGTTT
CAGTTGGATTGCCGAGTTCCTGGCTGGCTCTGGCTGTTTTCGTATGTGCCATGCTGTCCCTCGGCATGGGTAATGGAGCG
GTTTTTCAGCTCGTGCCCCAGCGTTTCCGCAAGGAGATCGGCGTGATGACGGGGCTGGTAGGCATGGCCGGCGGTGTAGG
CGGCTTCTATCTGGCGTCCAGTCTGGGCTATTCCAAACAGGTGAGCGGATCCTATATGCCCGGCTTTCTGATCTTTGCTG
GACTCGCACTCGCAGCCCTCGTCGGCCTGACCGCAGTGAAGAGGCGCTGGAGAACCACGTGGGGCGCCCCGCACCTGACC
ACCGCGAAGATCTGA

Upstream 100 bases:

>100_bases
CCGGGCAATCCCCGCTCCCTGGATGTCCCCTCGTCCGGTTCAAAGCGCGGTGCACGGGTCGAATTGCGGGATTGCAGGCG
CCCGCTCACTGGAGTCAAGA

Downstream 100 bases:

>100_bases
ATCTCGCATCCGTCGCGCGCGTGCAGGAGGTGTGGGTGGGCAACGGTTCGGCGAACGCTTCCACCCGCATTCTGAAGACA
TGGTGAAAGGTCGCTCGGTC

Product: putative nitrate transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MDKKSFLQAGHTPTLFAAFMYFDLAFMVWVMLGPLGVQIATDLGLTHAQKGMMVATPVLAGALLRFVMGMLVDHLKPKLT
GAIGQVIVIAALAYAWQHGIHSFEETLILGLFLGVAGASFAVALPLASRWYPPEHQGTALGIAGAGNSGTALAALFGPGL
AASFGWVNVFGLALIPLGLVFVAYLLMAKDAPECPPPKRLGEYLAVLKDKDAWWFMFFYSVTFGGFVGLASSLTIYFNTQ
YGLDAKMAGYFTAACVFAGSLVRPIGGNLADRIGGIKSLSVVYIAAAVALSIVSVGLPSSWLALAVFVCAMLSLGMGNGA
VFQLVPQRFRKEIGVMTGLVGMAGGVGGFYLASSLGYSKQVSGSYMPGFLIFAGLALAALVGLTAVKRRWRTTWGAPHLT
TAKI

Sequences:

>Translated_404_residues
MDKKSFLQAGHTPTLFAAFMYFDLAFMVWVMLGPLGVQIATDLGLTHAQKGMMVATPVLAGALLRFVMGMLVDHLKPKLT
GAIGQVIVIAALAYAWQHGIHSFEETLILGLFLGVAGASFAVALPLASRWYPPEHQGTALGIAGAGNSGTALAALFGPGL
AASFGWVNVFGLALIPLGLVFVAYLLMAKDAPECPPPKRLGEYLAVLKDKDAWWFMFFYSVTFGGFVGLASSLTIYFNTQ
YGLDAKMAGYFTAACVFAGSLVRPIGGNLADRIGGIKSLSVVYIAAAVALSIVSVGLPSSWLALAVFVCAMLSLGMGNGA
VFQLVPQRFRKEIGVMTGLVGMAGGVGGFYLASSLGYSKQVSGSYMPGFLIFAGLALAALVGLTAVKRRWRTTWGAPHLT
TAKI
>Mature_404_residues
MDKKSFLQAGHTPTLFAAFMYFDLAFMVWVMLGPLGVQIATDLGLTHAQKGMMVATPVLAGALLRFVMGMLVDHLKPKLT
GAIGQVIVIAALAYAWQHGIHSFEETLILGLFLGVAGASFAVALPLASRWYPPEHQGTALGIAGAGNSGTALAALFGPGL
AASFGWVNVFGLALIPLGLVFVAYLLMAKDAPECPPPKRLGEYLAVLKDKDAWWFMFFYSVTFGGFVGLASSLTIYFNTQ
YGLDAKMAGYFTAACVFAGSLVRPIGGNLADRIGGIKSLSVVYIAAAVALSIVSVGLPSSWLALAVFVCAMLSLGMGNGA
VFQLVPQRFRKEIGVMTGLVGMAGGVGGFYLASSLGYSKQVSGSYMPGFLIFAGLALAALVGLTAVKRRWRTTWGAPHLT
TAKI

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=402, Percent_Identity=27.6119402985075, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787743, Length=414, Percent_Identity=23.9130434782609, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42468; Mature: 42468

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]