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The map label for this gene is lguL [H]

Identifier: 119897517

GI number: 119897517

Start: 1320918

End: 1321319

Strand: Direct

Name: lguL [H]

Synonym: azo1226

Alternate gene names: 119897517

Gene position: 1320918-1321319 (Clockwise)

Preceding gene: 119897516

Following gene: 119897518

Centisome position: 30.19

GC content: 64.43

Gene sequence:

>402_bases
ATGCCAAAGCCAAAACTCATCCACACCATGATCCGGGTCCTGGACCTGGACAAATCACTGGCCTTCTACCGCGACGCCCT
CGGGCTGGAGGAGGCCTACCGGCTCGATTTTCCGGACTTCGCGCTGGCCTACCTGCGCAATGCCGAGAACGACTTCGAGC
TGGAACTCACGCTCAACAAGGGCCGCAGCGAGGCCTACACCCACGGTAGCGGCTACGGCCACATCGCGGTCTGTGTCGAC
GACGTCGAGGCCGAGCACCGCCGGCTTGCCGGGCTGGGGCTGGGGCCGACCGACGTCAAGCGTTTCGCCGATGGCGACGC
GCTGATCGCCCGCTTCTTCTTCATCCAGGACCCCGATGGCTACAAGGTCGAGGTGCTGGAGCGCCACGGCCATTACCAGT
GA

Upstream 100 bases:

>100_bases
GTGGCTGCAGGCGCCGCGGTGCCGAGGGGGCGGTCTCTCCTCCTACGACAAACCCGACGCGCGCGGGGCCTGCCTTCGAG
AACCACCGGGGGAGACGCCC

Downstream 100 bases:

>100_bases
CCGCGGCCCCCGCAGTCCGGCAGCACCCCCATACCAACAAGGAGAGAGACATCATGTCGGAACAATCCGTTGTCCTCGGC
GCACTCGCCGCGCCGCGCGT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MPKPKLIHTMIRVLDLDKSLAFYRDALGLEEAYRLDFPDFALAYLRNAENDFELELTLNKGRSEAYTHGSGYGHIAVCVD
DVEAEHRRLAGLGLGPTDVKRFADGDALIARFFFIQDPDGYKVEVLERHGHYQ

Sequences:

>Translated_133_residues
MPKPKLIHTMIRVLDLDKSLAFYRDALGLEEAYRLDFPDFALAYLRNAENDFELELTLNKGRSEAYTHGSGYGHIAVCVD
DVEAEHRRLAGLGLGPTDVKRFADGDALIARFFFIQDPDGYKVEVLERHGHYQ
>Mature_132_residues
PKPKLIHTMIRVLDLDKSLAFYRDALGLEEAYRLDFPDFALAYLRNAENDFELELTLNKGRSEAYTHGSGYGHIAVCVDD
VEAEHRRLAGLGLGPTDVKRFADGDALIARFFFIQDPDGYKVEVLERHGHYQ

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Escherichia coli, GI1787940, Length=128, Percent_Identity=35.15625, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15129; Mature: 14998

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKPKLIHTMIRVLDLDKSLAFYRDALGLEEAYRLDFPDFALAYLRNAENDFELELTLNK
CCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEEC
GRSEAYTHGSGYGHIAVCVDDVEAEHRRLAGLGLGPTDVKRFADGDALIARFFFIQDPDG
CCCCCEECCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHCCCHHEEEEEEEEECCCC
YKVEVLERHGHYQ
CEEEEEECCCCCC
>Mature Secondary Structure 
PKPKLIHTMIRVLDLDKSLAFYRDALGLEEAYRLDFPDFALAYLRNAENDFELELTLNK
CCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEEC
GRSEAYTHGSGYGHIAVCVDDVEAEHRRLAGLGLGPTDVKRFADGDALIARFFFIQDPDG
CCCCCEECCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHCCCHHEEEEEEEEECCCC
YKVEVLERHGHYQ
CEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565 [H]