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The map label for this gene is gloB1 [H]

Identifier: 119897499

GI number: 119897499

Start: 1301779

End: 1302555

Strand: Direct

Name: gloB1 [H]

Synonym: azo1208

Alternate gene names: 119897499

Gene position: 1301779-1302555 (Clockwise)

Preceding gene: 119897498

Following gene: 119897500

Centisome position: 29.75

GC content: 72.72

Gene sequence:

>777_bases
ATGAGGCCGCTGCCGCTGCGGGTGCGCCCGATTCCCGCCTTCCAGGACAACTACATCTGGCTGCTCACGCGCGGCGACGA
GGCGGTGGTGGTCGACCCCGGCACCGCCGGCCCGGTGCTCGCGGTGCTGACGGCCGAACGGCTGCGGTTGCGTGCGGTGC
TGATCACCCATCACCACGACGATCACGTGGGCGGCGTGGCGGAGGTGCTGCGCCACGCCGCGGTGCCGGTGTATGGCCCG
GCCGGCGAGGACATCGCGGTGCTCACCCATCGCGTCGCGGACGGCGAGCGTGTGGCGGTCCTCGACGACCATCCCGGTTT
CGAGGTGCTCGCGCTGCCCGGCCACACCGCGGGCCATATCGGCTATTTCGACGGCACCCACCTGTTCTGCGGCGACGTGC
TCTTCCCCTGTGGCTGCGGGCGGGTGTTCGAAGGCACGCCGGCGCAGATGCACGCCGCGCTCGCGCGCCTGGCCGCGCTG
CCGCCCGCCACCCAGGTGTATTGCGCGCACGAGTACAGCCTGGCTAATGCCCGCTTTGCGCTTGCCGCGGAGCCGGGCAA
CCGGCTGCTGCAGGGGCGCCAGCGCTGGATGGAGGCGCGCCGCGCCGCGGGCGAACCGACGGTGCCGTCCTCGCTGCGCG
ACGAGCTGGACACCAACCCCTTCCTGCGCTGCACCGAGCCCGCGCTGCGGCGCGCGGCGGCTGACTGGCGCGGCCACCCG
ATCGAGGACGAAGCCGAGGTGTTCGCGGCGCTGAGGGACTGGAAGAACCACTTCTGA

Upstream 100 bases:

>100_bases
CCGGCCGCGTGCGCGGCGCAAAGGCCTCGCTGCGCTCCGGTTTCGTCCTCGACGACCGCTTCTACTCGCGCGAGGAAGCC
GCGCAGCTGGCGGTGGCGGA

Downstream 100 bases:

>100_bases
TGAGTCCGCACGGGCCGCGCGCAAGCCGGCCCGTGCCGGTGCCGCGGGCCTGCGGCGCCAGCCGGATCCGGTCCGGGTGC
CCCGCTGCGCAAATCCTGTC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRPLPLRVRPIPAFQDNYIWLLTRGDEAVVVDPGTAGPVLAVLTAERLRLRAVLITHHHDDHVGGVAEVLRHAAVPVYGP
AGEDIAVLTHRVADGERVAVLDDHPGFEVLALPGHTAGHIGYFDGTHLFCGDVLFPCGCGRVFEGTPAQMHAALARLAAL
PPATQVYCAHEYSLANARFALAAEPGNRLLQGRQRWMEARRAAGEPTVPSSLRDELDTNPFLRCTEPALRRAAADWRGHP
IEDEAEVFAALRDWKNHF

Sequences:

>Translated_258_residues
MRPLPLRVRPIPAFQDNYIWLLTRGDEAVVVDPGTAGPVLAVLTAERLRLRAVLITHHHDDHVGGVAEVLRHAAVPVYGP
AGEDIAVLTHRVADGERVAVLDDHPGFEVLALPGHTAGHIGYFDGTHLFCGDVLFPCGCGRVFEGTPAQMHAALARLAAL
PPATQVYCAHEYSLANARFALAAEPGNRLLQGRQRWMEARRAAGEPTVPSSLRDELDTNPFLRCTEPALRRAAADWRGHP
IEDEAEVFAALRDWKNHF
>Mature_258_residues
MRPLPLRVRPIPAFQDNYIWLLTRGDEAVVVDPGTAGPVLAVLTAERLRLRAVLITHHHDDHVGGVAEVLRHAAVPVYGP
AGEDIAVLTHRVADGERVAVLDDHPGFEVLALPGHTAGHIGYFDGTHLFCGDVLFPCGCGRVFEGTPAQMHAALARLAAL
PPATQVYCAHEYSLANARFALAAEPGNRLLQGRQRWMEARRAAGEPTVPSSLRDELDTNPFLRCTEPALRRAAADWRGHP
IEDEAEVFAALRDWKNHF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=265, Percent_Identity=36.9811320754717, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI94538322, Length=265, Percent_Identity=36.9811320754717, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI14150041, Length=238, Percent_Identity=41.5966386554622, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI116642887, Length=264, Percent_Identity=36.3636363636364, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI21703352, Length=264, Percent_Identity=36.3636363636364, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI46361987, Length=207, Percent_Identity=41.5458937198068, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1786406, Length=257, Percent_Identity=43.1906614785992, Blast_Score=183, Evalue=9e-48,
Organism=Escherichia coli, GI1787158, Length=158, Percent_Identity=34.1772151898734, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=258, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320478, Length=268, Percent_Identity=30.2238805970149, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=283, Percent_Identity=28.6219081272085, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356335, Length=242, Percent_Identity=40.495867768595, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24667711, Length=242, Percent_Identity=40.495867768595, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24667703, Length=242, Percent_Identity=40.495867768595, Blast_Score=174, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28219; Mature: 28219

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLPLRVRPIPAFQDNYIWLLTRGDEAVVVDPGTAGPVLAVLTAERLRLRAVLITHHHD
CCCCCCEEEECCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHEEEEEEEEECCC
DHVGGVAEVLRHAAVPVYGPAGEDIAVLTHRVADGERVAVLDDHPGFEVLALPGHTAGHI
CCCCHHHHHHHHCCCCEECCCCCCCEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCE
GYFDGTHLFCGDVLFPCGCGRVFEGTPAQMHAALARLAALPPATQVYCAHEYSLANARFA
EECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECEECCCCEEE
LAAEPGNRLLQGRQRWMEARRAAGEPTVPSSLRDELDTNPFLRCTEPALRRAAADWRGHP
EEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHCCCCC
IEDEAEVFAALRDWKNHF
CCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPLPLRVRPIPAFQDNYIWLLTRGDEAVVVDPGTAGPVLAVLTAERLRLRAVLITHHHD
CCCCCCEEEECCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHEEEEEEEEECCC
DHVGGVAEVLRHAAVPVYGPAGEDIAVLTHRVADGERVAVLDDHPGFEVLALPGHTAGHI
CCCCHHHHHHHHCCCCEECCCCCCCEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCE
GYFDGTHLFCGDVLFPCGCGRVFEGTPAQMHAALARLAALPPATQVYCAHEYSLANARFA
EECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECEECCCCEEE
LAAEPGNRLLQGRQRWMEARRAAGEPTVPSSLRDELDTNPFLRCTEPALRRAAADWRGHP
EEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHCCCCC
IEDEAEVFAALRDWKNHF
CCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA