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The map label for this gene is dmlR [H]

Identifier: 119897452

GI number: 119897452

Start: 1249955

End: 1250920

Strand: Direct

Name: dmlR [H]

Synonym: azo1161

Alternate gene names: 119897452

Gene position: 1249955-1250920 (Clockwise)

Preceding gene: 119897449

Following gene: 119897455

Centisome position: 28.56

GC content: 69.88

Gene sequence:

>966_bases
ATGGATCAGCTGAAGCAGATCGAAACCTTCGTTGCCGTCGCCAGTCGCGGCAGCCTGTCGGCCGCGGCGCGGGCAGAAGG
CGTGACCCCGGCGGTGATCGGCCGTCGGCTCGACGGGCTGGAAGCCCGGCTGGGCGTGCGGCTGCTGGTGCGCACCACGC
GCAGCATCACGCTCACCTTCGAAGGCAGCGCCTTCCTGGAGGACTGCCAGCGCATCCTCAACGATCTCTCCAACGCCGAG
GCCTCGGTCAGCCTCGGCGGCGTCAAGCCGAGCGGGCATCTGCGCGTCTCCGCGCCTGCCGGTTTCGGCCGCCGCCACGT
CGCGCCGCTGGTGCGCGAATTCCTGCTCGCGAATCCCGACGTCAGCTGCAGCCTCGACCTCTCCGACCGCCTCGTCGACC
TGGTCAACGAAGGGCTGGATTGCGCCGTGCGCATCGGCGACATGGCGGATTCCAGCCTGGTGTCGGTGAAGCTGGCCGAC
AACCGCCGCGTGGTGGTGGCCAGCCCCGACTATCTTGCCCGCCACGGCGTGCCGCGCACGCCGGAGGATCTGCCGCGCCA
CGTCTGCCTTACGCTGGTGCAGCAGCGCGGCTGGCAATTCGCCGACGGCCGCGGCGGCGCGGTCTCGGCCAAGGTGGCGG
GCCGGCTGGAGTGCAACGACGGCGCGGTGCTGCACGAATGGGCGCTGGCCGGGCAGGGCCTGGCGTGGCGATCCTTGTGG
GAAGTGTCCGAGGACCTCGCCGCGGGCCGGCTGGTGTCGGTGCTGGACGAATTCGCCGCGCGCCCCACCGGCATCTACGC
GGTCTTTCCGTACCGCCGCAACCTTGCGCTGCGGGTGCGCTACTTCATCGATTACCTCAAGCAGCGCTATGGGGATACGG
GGTACTGGAATTCGGCTGCCGGCGAGCCGCTCACCCCACCCACACCGCCCGAAAGTCATTCACGTTCGTCAGCGTCGGCC
CGGTAA

Upstream 100 bases:

>100_bases
ATCGGGATGTCTCCTCCCCCGAATGAGCTGCAGTCTAGATCGGACCTAAATAAAACAGAAGAAACGGTATGATCACTTTA
TCTTTTACTAAAAGTACAAA

Downstream 100 bases:

>100_bases
CCAGCGAATCGCCCAAGGCCTCGAAGAAGCCATGGCCGTCGTTGTCGTCCAGCCGGTCGCGCGGGCGCAGGCCGGCCGCC
CAGGCGCGCGCCAGCGTATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDQLKQIETFVAVASRGSLSAAARAEGVTPAVIGRRLDGLEARLGVRLLVRTTRSITLTFEGSAFLEDCQRILNDLSNAE
ASVSLGGVKPSGHLRVSAPAGFGRRHVAPLVREFLLANPDVSCSLDLSDRLVDLVNEGLDCAVRIGDMADSSLVSVKLAD
NRRVVVASPDYLARHGVPRTPEDLPRHVCLTLVQQRGWQFADGRGGAVSAKVAGRLECNDGAVLHEWALAGQGLAWRSLW
EVSEDLAAGRLVSVLDEFAARPTGIYAVFPYRRNLALRVRYFIDYLKQRYGDTGYWNSAAGEPLTPPTPPESHSRSSASA
R

Sequences:

>Translated_321_residues
MDQLKQIETFVAVASRGSLSAAARAEGVTPAVIGRRLDGLEARLGVRLLVRTTRSITLTFEGSAFLEDCQRILNDLSNAE
ASVSLGGVKPSGHLRVSAPAGFGRRHVAPLVREFLLANPDVSCSLDLSDRLVDLVNEGLDCAVRIGDMADSSLVSVKLAD
NRRVVVASPDYLARHGVPRTPEDLPRHVCLTLVQQRGWQFADGRGGAVSAKVAGRLECNDGAVLHEWALAGQGLAWRSLW
EVSEDLAAGRLVSVLDEFAARPTGIYAVFPYRRNLALRVRYFIDYLKQRYGDTGYWNSAAGEPLTPPTPPESHSRSSASA
R
>Mature_321_residues
MDQLKQIETFVAVASRGSLSAAARAEGVTPAVIGRRLDGLEARLGVRLLVRTTRSITLTFEGSAFLEDCQRILNDLSNAE
ASVSLGGVKPSGHLRVSAPAGFGRRHVAPLVREFLLANPDVSCSLDLSDRLVDLVNEGLDCAVRIGDMADSSLVSVKLAD
NRRVVVASPDYLARHGVPRTPEDLPRHVCLTLVQQRGWQFADGRGGAVSAKVAGRLECNDGAVLHEWALAGQGLAWRSLW
EVSEDLAAGRLVSVLDEFAARPTGIYAVFPYRRNLALRVRYFIDYLKQRYGDTGYWNSAAGEPLTPPTPPESHSRSSASA
R

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=39.5904436860068, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1789440, Length=302, Percent_Identity=34.1059602649007, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=32.9861111111111, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=32.3024054982818, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=30.6896551724138, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=30.4054054054054, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1787589, Length=294, Percent_Identity=29.2517006802721, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789173, Length=265, Percent_Identity=28.3018867924528, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788706, Length=201, Percent_Identity=25.8706467661692, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=26.7123287671233, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=23.5690235690236, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34756; Mature: 34756

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQLKQIETFVAVASRGSLSAAARAEGVTPAVIGRRLDGLEARLGVRLLVRTTRSITLTF
CCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHCCHHHHHHHHEEEEECCEEEEEE
EGSAFLEDCQRILNDLSNAEASVSLGGVKPSGHLRVSAPAGFGRRHVAPLVREFLLANPD
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
VSCSLDLSDRLVDLVNEGLDCAVRIGDMADSSLVSVKLADNRRVVVASPDYLARHGVPRT
CEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCCHHHHCCCCCC
PEDLPRHVCLTLVQQRGWQFADGRGGAVSAKVAGRLECNDGAVLHEWALAGQGLAWRSLW
HHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEECCCCCEEEHHHHCCCCCHHHHHH
EVSEDLAAGRLVSVLDEFAARPTGIYAVFPYRRNLALRVRYFIDYLKQRYGDTGYWNSAA
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
GEPLTPPTPPESHSRSSASAR
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDQLKQIETFVAVASRGSLSAAARAEGVTPAVIGRRLDGLEARLGVRLLVRTTRSITLTF
CCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHCCHHHHHHHHEEEEECCEEEEEE
EGSAFLEDCQRILNDLSNAEASVSLGGVKPSGHLRVSAPAGFGRRHVAPLVREFLLANPD
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
VSCSLDLSDRLVDLVNEGLDCAVRIGDMADSSLVSVKLADNRRVVVASPDYLARHGVPRT
CEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCCHHHHCCCCCC
PEDLPRHVCLTLVQQRGWQFADGRGGAVSAKVAGRLECNDGAVLHEWALAGQGLAWRSLW
HHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEECCCCCEEEHHHHCCCCCHHHHHH
EVSEDLAAGRLVSVLDEFAARPTGIYAVFPYRRNLALRVRYFIDYLKQRYGDTGYWNSAA
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
GEPLTPPTPPESHSRSSASAR
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]