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The map label for this gene is livJ [C]

Identifier: 119897352

GI number: 119897352

Start: 1140857

End: 1142011

Strand: Reverse

Name: livJ [C]

Synonym: azo1061

Alternate gene names: 119897352

Gene position: 1142011-1140857 (Counterclockwise)

Preceding gene: 119897353

Following gene: 119897350

Centisome position: 26.1

GC content: 68.4

Gene sequence:

>1155_bases
ATGACATCCCTGCGTCTGCCCGCGCTCTTTCGCCGCGGACTCGCGTCCCTGCTCGCCGTTGCGGCGACATGCGGTGCGCC
TGCCGCCTTCGCCCAGCAGGGCGTAGACGCCGACGCCATCACGCTGGGTCACACGGGCGCCCTCAGCGGCCCGCTCGCCG
AACTCAACAAGGAATACCTGGCCGGCGCGAACCTGTATTTCAACCAGACCAACGAGCGCGGCGGCATCAACGGACGGCGG
ATCCGGCTGCTCACGCTCGACGATGCCTACGATCCCAACAAGGCGGCCGAGAACGCGCAGCGGCTGATCGAACAGCATCA
GGTTTTCGCGCTCTTCGCCTGCTTCGGCACCGGCCCCAGCCTCAAGGTCGTGCCGCTGGCCGCGGCCGCCAAGGTCCCGT
TCTTCGCCCCCTACACCGGCGCGGAAGCGCTGCGCGAGCCGCTCAACCCCTACGTATTCCACGTCCGCGCGTCGTACCGG
CAGGAGGTCGAGAAGGCGGTGGACCACCTCACGAAGCTGGGGGTGAAGTCGATCGGGGTTGTCCACCACGCCGACCCCTT
CGGCCAGGCCGGCCTCGACGCAGCGGTCGCATCGCTCGCCAAGCGCAATCTGACGCCGGCAGTGGTCGCGCCGATCGCTT
CCAGCGGTGCGGACGCGGCGGACACGGTGAAGAAGGTCGCCGCGGCAAACCCGGCCGCGGTCATCATGATCACCGCCGGC
AACAGTTCCGCCGCCCTGCTGCGCGCACTGCAGCAGTCAGGCAGCCAGCCGATGCTCTACGGGCTGTCGGTGATCAGCAG
CACCCAGCTCATCCGCGAACTCGGCGAGAAGGCCCACGGCCTGGTGATCGCGCAGGTGATGCCCTCGCCTTTCCGGGTCG
ACTATCCCTTCGTGCGCGATTACCGCCAGGCGGCCGAGAAAGCGGGCATCGCCTACTCGTACGCGTCGCTGGAGGGCTAT
CTCGCGGCGCGCAGTTTCGGCGAAGCCGTGCGCCGCGCCGGACGCGATCTCACCCGCGAAAAGCTGATCAGCGCCCTGGA
GAACATGGGCGACTGGGACGCCGGCGGCATGCGCATGGCGTTCTCGCCGCGGCGCCATGTGGGCATGGATTATGTGGACC
TGACGGTGATCAGCCGCGGCAACTTCACCCGCTGA

Upstream 100 bases:

>100_bases
TGAGGAGCGCAGCCGCTGCCGGCGGCACCGCCAGCGCGGCTGGCAGCAACAACAACGATCGCCGCTCCTCGCCACACGCT
AAACCGGAGAGGAGAAAACG

Downstream 100 bases:

>100_bases
CGGCGCACCGCGACTACACCACGCACCATGACAACGGCCCCGTCCAGCGGGGCCGTTGTCGTTTTCAGGCGCGGCGCCAG
GTGGTGCCTCCCGCGCCGTC

Product: extracellular ligand binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTSLRLPALFRRGLASLLAVAATCGAPAAFAQQGVDADAITLGHTGALSGPLAELNKEYLAGANLYFNQTNERGGINGRR
IRLLTLDDAYDPNKAAENAQRLIEQHQVFALFACFGTGPSLKVVPLAAAAKVPFFAPYTGAEALREPLNPYVFHVRASYR
QEVEKAVDHLTKLGVKSIGVVHHADPFGQAGLDAAVASLAKRNLTPAVVAPIASSGADAADTVKKVAAANPAAVIMITAG
NSSAALLRALQQSGSQPMLYGLSVISSTQLIRELGEKAHGLVIAQVMPSPFRVDYPFVRDYRQAAEKAGIAYSYASLEGY
LAARSFGEAVRRAGRDLTREKLISALENMGDWDAGGMRMAFSPRRHVGMDYVDLTVISRGNFTR

Sequences:

>Translated_384_residues
MTSLRLPALFRRGLASLLAVAATCGAPAAFAQQGVDADAITLGHTGALSGPLAELNKEYLAGANLYFNQTNERGGINGRR
IRLLTLDDAYDPNKAAENAQRLIEQHQVFALFACFGTGPSLKVVPLAAAAKVPFFAPYTGAEALREPLNPYVFHVRASYR
QEVEKAVDHLTKLGVKSIGVVHHADPFGQAGLDAAVASLAKRNLTPAVVAPIASSGADAADTVKKVAAANPAAVIMITAG
NSSAALLRALQQSGSQPMLYGLSVISSTQLIRELGEKAHGLVIAQVMPSPFRVDYPFVRDYRQAAEKAGIAYSYASLEGY
LAARSFGEAVRRAGRDLTREKLISALENMGDWDAGGMRMAFSPRRHVGMDYVDLTVISRGNFTR
>Mature_383_residues
TSLRLPALFRRGLASLLAVAATCGAPAAFAQQGVDADAITLGHTGALSGPLAELNKEYLAGANLYFNQTNERGGINGRRI
RLLTLDDAYDPNKAAENAQRLIEQHQVFALFACFGTGPSLKVVPLAAAAKVPFFAPYTGAEALREPLNPYVFHVRASYRQ
EVEKAVDHLTKLGVKSIGVVHHADPFGQAGLDAAVASLAKRNLTPAVVAPIASSGADAADTVKKVAAANPAAVIMITAGN
SSAALLRALQQSGSQPMLYGLSVISSTQLIRELGEKAHGLVIAQVMPSPFRVDYPFVRDYRQAAEKAGIAYSYASLEGYL
AARSFGEAVRRAGRDLTREKLISALENMGDWDAGGMRMAFSPRRHVGMDYVDLTVISRGNFTR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40867; Mature: 40736

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLRLPALFRRGLASLLAVAATCGAPAAFAQQGVDADAITLGHTGALSGPLAELNKEYL
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHH
AGANLYFNQTNERGGINGRRIRLLTLDDAYDPNKAAENAQRLIEQHQVFALFACFGTGPS
CCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
LKVVPLAAAAKVPFFAPYTGAEALREPLNPYVFHVRASYRQEVEKAVDHLTKLGVKSIGV
CEEEEEHHHCCCCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCHHHHCE
VHHADPFGQAGLDAAVASLAKRNLTPAVVAPIASSGADAADTVKKVAAANPAAVIMITAG
EECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECC
NSSAALLRALQQSGSQPMLYGLSVISSTQLIRELGEKAHGLVIAQVMPSPFRVDYPFVRD
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHH
YRQAAEKAGIAYSYASLEGYLAARSFGEAVRRAGRDLTREKLISALENMGDWDAGGMRMA
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
FSPRRHVGMDYVDLTVISRGNFTR
ECCCHHCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
TSLRLPALFRRGLASLLAVAATCGAPAAFAQQGVDADAITLGHTGALSGPLAELNKEYL
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHH
AGANLYFNQTNERGGINGRRIRLLTLDDAYDPNKAAENAQRLIEQHQVFALFACFGTGPS
CCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
LKVVPLAAAAKVPFFAPYTGAEALREPLNPYVFHVRASYRQEVEKAVDHLTKLGVKSIGV
CEEEEEHHHCCCCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCHHHHCE
VHHADPFGQAGLDAAVASLAKRNLTPAVVAPIASSGADAADTVKKVAAANPAAVIMITAG
EECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECC
NSSAALLRALQQSGSQPMLYGLSVISSTQLIRELGEKAHGLVIAQVMPSPFRVDYPFVRD
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHH
YRQAAEKAGIAYSYASLEGYLAARSFGEAVRRAGRDLTREKLISALENMGDWDAGGMRMA
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
FSPRRHVGMDYVDLTVISRGNFTR
ECCCHHCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]