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The map label for this gene is hcaT [H]

Identifier: 119897328

GI number: 119897328

Start: 1114958

End: 1116148

Strand: Direct

Name: hcaT [H]

Synonym: azo1037

Alternate gene names: 119897328

Gene position: 1114958-1116148 (Clockwise)

Preceding gene: 119897327

Following gene: 119897329

Centisome position: 25.48

GC content: 67.84

Gene sequence:

>1191_bases
GTGACGACGGCAGGCGGCAGCGGGGCGGGCCTTCCTTACTGGCGGCTGTCCGCGTACTACTTCAGCTATTTCGCCTTTGT
CGGGGCGTTTTCGCCCTACTTCACGCTCTACCTCAAGGCGCTCGATTTTTCCGCGACCGAAATCGCCGTGCTGATGTCGC
TGATGCAGGTGATGCGGGTGCTGGCGCCCAACCTGTGGGGCTGGCTCGCCGAACACCTCGGGGTGCGCCTGCCCATCGTC
CGCCTGTCCGCGCTGATGAGCGTGGCCGGCTTTGCGGTGTTCTTCTTCACCGAGTCGTTCAGCGGCATCTTTGCCGGCAT
GGCGCTGATGTCCTTCTTCTGGGCTGCGGCGCTGCCGCTGGTGGAAAGCCTCACCTTTGCTCACCTGGCCACCGCCGCGC
ATCGGTACGGCAGCATCCGAGCGTGGGGATCGGTCGGCTTCATCGCCGCGGTGGTCGGTCTGGGCGCCTTGCTCGACCTG
CTGCCGATCCGCGCGGTGCTGTGGGGGACGACGGCGGTGCTCGGCGCCATTCTCGTCTGTTCGCTGTTGCTGCCCGAAGC
GGCGCGGGCCGCCACCCGGGGCGCCGGGGCGAGCCTGCGCGAGGTTCTCGGGCGAAGTGAAGTCAAGGCGCTGCTCGGTG
CCTGCTTCCTGATGTCGGCGGCCCACGGTGCGTTCTACGTGTTCTTTTCCATCCTGCTGGTCGATCACGGCTACGGCAAG
GCGGCGGTGGGCGCCTTGTGGGCGCTCGGCGTGGTGTCGGAGATCATCGTGTTCATGTTCATGCCGCGGTTGATGCGGCG
GGTTCCGCTGCGCGCCATCCTGTTGTTTGCATTCTGCTGCGCTGTCGTGCGCTTTGTGCTGACCGGCTGGGGCGCGGCCA
GCGTGGCGGTGGTGGCGGCCGCCCAGGTGCTTCACGGCGTGACCTTCGGTGCTTACCATGCGGCCGCGATTGCCGCCATC
AACCGCTGGTTCTCCGGGCGCCTGCAGGCGCGCGGGCAGGCCTTGTATGGCAGCCTTTCATTCGGCGCCGGCGGCATGCT
GGGCGGCTTGGTGAGCGGCCTCGGCTGGGAAACGATAGGACCGGCCTGGACCTTCACGCTCGGCTCGGTGTTTGCGCTGG
CCGGGCTGCTATGGTTGAAACTGGGTTGGCGCCCGGGCCTCGGCGACGAGGTGGCGGGCGCCCGCGCATAG

Upstream 100 bases:

>100_bases
TGCGTCACCGCGCCCAGTGCGGCGACGTCCGTACCGCCACGCCGCAGATTGCCGCGCTGGCGCCCGAGGGCGTGCAGGCG
GTGCCGTCGCCACGCGCGGA

Downstream 100 bases:

>100_bases
GCGGAGGAGCAAGCATGAAGTTCTTGACGCAAAGGGTTTTGCCGGCCGCGCTGGCGGTCACCATGGCGGTCGCCTGCCAG
ACGGTGCAGACGACCGGGGG

Product: putative MFS metabolite transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTTAGGSGAGLPYWRLSAYYFSYFAFVGAFSPYFTLYLKALDFSATEIAVLMSLMQVMRVLAPNLWGWLAEHLGVRLPIV
RLSALMSVAGFAVFFFTESFSGIFAGMALMSFFWAAALPLVESLTFAHLATAAHRYGSIRAWGSVGFIAAVVGLGALLDL
LPIRAVLWGTTAVLGAILVCSLLLPEAARAATRGAGASLREVLGRSEVKALLGACFLMSAAHGAFYVFFSILLVDHGYGK
AAVGALWALGVVSEIIVFMFMPRLMRRVPLRAILLFAFCCAVVRFVLTGWGAASVAVVAAAQVLHGVTFGAYHAAAIAAI
NRWFSGRLQARGQALYGSLSFGAGGMLGGLVSGLGWETIGPAWTFTLGSVFALAGLLWLKLGWRPGLGDEVAGARA

Sequences:

>Translated_396_residues
MTTAGGSGAGLPYWRLSAYYFSYFAFVGAFSPYFTLYLKALDFSATEIAVLMSLMQVMRVLAPNLWGWLAEHLGVRLPIV
RLSALMSVAGFAVFFFTESFSGIFAGMALMSFFWAAALPLVESLTFAHLATAAHRYGSIRAWGSVGFIAAVVGLGALLDL
LPIRAVLWGTTAVLGAILVCSLLLPEAARAATRGAGASLREVLGRSEVKALLGACFLMSAAHGAFYVFFSILLVDHGYGK
AAVGALWALGVVSEIIVFMFMPRLMRRVPLRAILLFAFCCAVVRFVLTGWGAASVAVVAAAQVLHGVTFGAYHAAAIAAI
NRWFSGRLQARGQALYGSLSFGAGGMLGGLVSGLGWETIGPAWTFTLGSVFALAGLLWLKLGWRPGLGDEVAGARA
>Mature_395_residues
TTAGGSGAGLPYWRLSAYYFSYFAFVGAFSPYFTLYLKALDFSATEIAVLMSLMQVMRVLAPNLWGWLAEHLGVRLPIVR
LSALMSVAGFAVFFFTESFSGIFAGMALMSFFWAAALPLVESLTFAHLATAAHRYGSIRAWGSVGFIAAVVGLGALLDLL
PIRAVLWGTTAVLGAILVCSLLLPEAARAATRGAGASLREVLGRSEVKALLGACFLMSAAHGAFYVFFSILLVDHGYGKA
AVGALWALGVVSEIIVFMFMPRLMRRVPLRAILLFAFCCAVVRFVLTGWGAASVAVVAAAQVLHGVTFGAYHAAAIAAIN
RWFSGRLQARGQALYGSLSFGAGGMLGGLVSGLGWETIGPAWTFTLGSVFALAGLLWLKLGWRPGLGDEVAGARA

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=356, Percent_Identity=27.8089887640449, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788415, Length=357, Percent_Identity=23.249299719888, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 41862; Mature: 41731

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTAGGSGAGLPYWRLSAYYFSYFAFVGAFSPYFTLYLKALDFSATEIAVLMSLMQVMRV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH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CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]