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The map label for this gene is ilvB [H]

Identifier: 119897325

GI number: 119897325

Start: 1111730

End: 1113397

Strand: Direct

Name: ilvB [H]

Synonym: azo1034

Alternate gene names: 119897325

Gene position: 1111730-1113397 (Clockwise)

Preceding gene: 119897324

Following gene: 119897326

Centisome position: 25.4

GC content: 66.73

Gene sequence:

>1668_bases
ATGAACCAGATGACCGGCGCAGAACTGATCGTGCGCCTGCTCGAGCGCCAGGGCGTGCGCACCATTGCCGGGATTCCCGG
CGGCGCGATCCTGCCGCTCTATGACGCGCTCTCGCAAAGCGACCTGATCGAACACGTGCTGGCCCGCCACGAACAAGGCG
CAGGCTTCATGGCCCAGGGTATGGCGCGGGTGTCCGGCGTGCCGGGCGTGTGCTTTGCCTCCAGCGGTCCTGGCGCCACG
AACCTTGTCACCGCCATCGCGGACGCCCAACTCGATTCGATCCCGATGGTGGCGATCACCGGTCAGGTGCCGCTGCCGAT
GATCGGCACCGATGCTTTCCAGGAAGTCGATATCTACGGCATGACGGTGCCGATCACCAAGCACAACTTCCTGGTGCGTT
CGGCCGAAGAACTGCTGAAGGTGATTCCCGAGGCGTTTCGCATCGCGATGTCCGGGCGCCCGGGACCCGTGCTGGTCGAC
GTGCCCAAGGACGTGCAGAACCAGCGCATCGAGTTCGCCGAGTTTCCGCCGCCGGCGGGCCGCGACGCGGTGCCGGCGCT
CGACGCCGCGGCGATCGACCGTGCCGCCGCGATGATCAACGAAGCCGAACGTCCGGTGCTCTACCTCGGCGGTGGCGTCA
TCCACTCTGGCGCTTCGGCGCAGGCGGTCACGCTGGCCGAGCAGGCGGGCCTGCCGACCACGATGACGCTGATGGCGCTC
GGGGCGATGGCGGTGGACCACCCGCTGTCCATCGGCATGCTCGGAATGCACGGTGCCCGCTATACCAACTACGTGCTCGA
AGAGGCTGACCTGCTGGTCTGTGTCGGCGCGCGCTTCGACGACCGCGCGATCGGCCGGGCGGCGCAGTTCTGTCCCGGCG
CCCGCATCGTTCATATCGACGTCGATCCGTCCGAGCTGCACAAGATCAAGACCGCACACGTGGCGATCCACGGCGACGTC
GCGGCGGTGCTCGATGCGCTGTTGCCGAAGGTGAAGGTGCAACTGCGCAAGCGCTGGCTGTCCCACGTCGAAAGCCTGAA
GTCCCGGTTCCCGATGCAGATGCCGGACCTCGACGATCCGCGCAGCCATTACGGCCTGATCCATGCCGTGGCGGCGGCCC
TCGACGACGAGGCGGTGATCGCCACCGATGTCGGCCAGCATCAGATGTGGGTGGCGCAGGCCTATCCGTTCCGGCGCGCG
CGCCAGTGGCTCACGTCCGGCGGGCTGGGCACGATGGGTTTCGGTATGCCGACCGCGATCGGTGCGGCGCTGGCCGCGCC
CGAACGCACCGTGGTGTGTTTTTCCGGTGATGGCAGCCTGCAGATGAACATCCAGGAACTGGCCACGCTGGCCGAGCTGG
GGCTCAACGTCAAGATCGTGCTGATGAACAACAATTCGCTCGGCTTGGTGTACCAGCAACAGAACCTGTTCTACGGCAAG
CGCACCTTCGCGTCCAAGTATCGCGGTGGTCCGGACTTCTGCCGCATTGCCGAGGGCTATGGCATCGCCGCGGTCGATCT
CGACGCGAGCGACAACCCCCGCGCGACGCTGGCCGAAGCCCTGCAGGCGCCGGGGCCATGCCTGATCCATGCCTCGATCG
ACCGCGAGCAGTTTGTCTACCCCATGGTGCCGCCGGGCGCCGCCAACACCGAAATGATCGGAGGCTGA

Upstream 100 bases:

>100_bases
CACAGCATTCCCCAACCCCCGCCGGCGCGAAATGCCGGCGGGGGTTTTTGTTTTTCTGGCCGCCGGCATCAAGCTCCGAA
CCTGATCGAAGGAGATGACG

Downstream 100 bases:

>100_bases
TGATGATCGAACAGGTTGCCGACCCGCTGCCGCAAGCGGGCTTTGCCAAGGTTGTGCTCGAACTGGAGGTGCGCAACCAC
CCCGGCGTGATGAGCCATAT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MNQMTGAELIVRLLERQGVRTIAGIPGGAILPLYDALSQSDLIEHVLARHEQGAGFMAQGMARVSGVPGVCFASSGPGAT
NLVTAIADAQLDSIPMVAITGQVPLPMIGTDAFQEVDIYGMTVPITKHNFLVRSAEELLKVIPEAFRIAMSGRPGPVLVD
VPKDVQNQRIEFAEFPPPAGRDAVPALDAAAIDRAAAMINEAERPVLYLGGGVIHSGASAQAVTLAEQAGLPTTMTLMAL
GAMAVDHPLSIGMLGMHGARYTNYVLEEADLLVCVGARFDDRAIGRAAQFCPGARIVHIDVDPSELHKIKTAHVAIHGDV
AAVLDALLPKVKVQLRKRWLSHVESLKSRFPMQMPDLDDPRSHYGLIHAVAAALDDEAVIATDVGQHQMWVAQAYPFRRA
RQWLTSGGLGTMGFGMPTAIGAALAAPERTVVCFSGDGSLQMNIQELATLAELGLNVKIVLMNNNSLGLVYQQQNLFYGK
RTFASKYRGGPDFCRIAEGYGIAAVDLDASDNPRATLAEALQAPGPCLIHASIDREQFVYPMVPPGAANTEMIGG

Sequences:

>Translated_555_residues
MNQMTGAELIVRLLERQGVRTIAGIPGGAILPLYDALSQSDLIEHVLARHEQGAGFMAQGMARVSGVPGVCFASSGPGAT
NLVTAIADAQLDSIPMVAITGQVPLPMIGTDAFQEVDIYGMTVPITKHNFLVRSAEELLKVIPEAFRIAMSGRPGPVLVD
VPKDVQNQRIEFAEFPPPAGRDAVPALDAAAIDRAAAMINEAERPVLYLGGGVIHSGASAQAVTLAEQAGLPTTMTLMAL
GAMAVDHPLSIGMLGMHGARYTNYVLEEADLLVCVGARFDDRAIGRAAQFCPGARIVHIDVDPSELHKIKTAHVAIHGDV
AAVLDALLPKVKVQLRKRWLSHVESLKSRFPMQMPDLDDPRSHYGLIHAVAAALDDEAVIATDVGQHQMWVAQAYPFRRA
RQWLTSGGLGTMGFGMPTAIGAALAAPERTVVCFSGDGSLQMNIQELATLAELGLNVKIVLMNNNSLGLVYQQQNLFYGK
RTFASKYRGGPDFCRIAEGYGIAAVDLDASDNPRATLAEALQAPGPCLIHASIDREQFVYPMVPPGAANTEMIGG
>Mature_555_residues
MNQMTGAELIVRLLERQGVRTIAGIPGGAILPLYDALSQSDLIEHVLARHEQGAGFMAQGMARVSGVPGVCFASSGPGAT
NLVTAIADAQLDSIPMVAITGQVPLPMIGTDAFQEVDIYGMTVPITKHNFLVRSAEELLKVIPEAFRIAMSGRPGPVLVD
VPKDVQNQRIEFAEFPPPAGRDAVPALDAAAIDRAAAMINEAERPVLYLGGGVIHSGASAQAVTLAEQAGLPTTMTLMAL
GAMAVDHPLSIGMLGMHGARYTNYVLEEADLLVCVGARFDDRAIGRAAQFCPGARIVHIDVDPSELHKIKTAHVAIHGDV
AAVLDALLPKVKVQLRKRWLSHVESLKSRFPMQMPDLDDPRSHYGLIHAVAAALDDEAVIATDVGQHQMWVAQAYPFRRA
RQWLTSGGLGTMGFGMPTAIGAALAAPERTVVCFSGDGSLQMNIQELATLAELGLNVKIVLMNNNSLGLVYQQQNLFYGK
RTFASKYRGGPDFCRIAEGYGIAAVDLDASDNPRATLAEALQAPGPCLIHASIDREQFVYPMVPPGAANTEMIGG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=492, Percent_Identity=27.8455284552846, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI21361361, Length=516, Percent_Identity=26.1627906976744, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=64.0144665461121, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=40.8127208480565, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=34.6715328467153, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=27.8688524590164, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=25.673249551167, Blast_Score=145, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=28.1138790035587, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=28.1138790035587, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=568, Percent_Identity=26.2323943661972, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=40.9722222222222, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=23.1707317073171, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=518, Percent_Identity=20.6563706563707, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=514, Percent_Identity=21.9844357976654, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=556, Percent_Identity=20.6834532374101, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19922626, Length=486, Percent_Identity=27.1604938271605, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59039; Mature: 59039

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQMTGAELIVRLLERQGVRTIAGIPGGAILPLYDALSQSDLIEHVLARHEQGAGFMAQG
CCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHH
MARVSGVPGVCFASSGPGATNLVTAIADAQLDSIPMVAITGQVPLPMIGTDAFQEVDIYG
HHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHEEEEEE
MTVPITKHNFLVRSAEELLKVIPEAFRIAMSGRPGPVLVDVPKDVQNQRIEFAEFPPPAG
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCCC
RDAVPALDAAAIDRAAAMINEAERPVLYLGGGVIHSGASAQAVTLAEQAGLPTTMTLMAL
CCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCEEEEHHHCCCCHHHHHHHH
GAMAVDHPLSIGMLGMHGARYTNYVLEEADLLVCVGARFDDRAIGRAAQFCPGARIVHID
HHHHHCCCCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEE
VDPSELHKIKTAHVAIHGDVAAVLDALLPKVKVQLRKRWLSHVESLKSRFPMQMPDLDDP
CCHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RSHYGLIHAVAAALDDEAVIATDVGQHQMWVAQAYPFRRARQWLTSGGLGTMGFGMPTAI
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHCCHHHHHHHHHCCCCCCCCCCCHHHH
GAALAAPERTVVCFSGDGSLQMNIQELATLAELGLNVKIVLMNNNSLGLVYQQQNLFYGK
HHHHCCCCCEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCEECH
RTFASKYRGGPDFCRIAEGYGIAAVDLDASDNPRATLAEALQAPGPCLIHASIDREQFVY
HHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEE
PMVPPGAANTEMIGG
ECCCCCCCCCCCCCC
>Mature Secondary Structure
MNQMTGAELIVRLLERQGVRTIAGIPGGAILPLYDALSQSDLIEHVLARHEQGAGFMAQG
CCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHH
MARVSGVPGVCFASSGPGATNLVTAIADAQLDSIPMVAITGQVPLPMIGTDAFQEVDIYG
HHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHEEEEEE
MTVPITKHNFLVRSAEELLKVIPEAFRIAMSGRPGPVLVDVPKDVQNQRIEFAEFPPPAG
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCCC
RDAVPALDAAAIDRAAAMINEAERPVLYLGGGVIHSGASAQAVTLAEQAGLPTTMTLMAL
CCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCEEEEHHHCCCCHHHHHHHH
GAMAVDHPLSIGMLGMHGARYTNYVLEEADLLVCVGARFDDRAIGRAAQFCPGARIVHID
HHHHHCCCCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEE
VDPSELHKIKTAHVAIHGDVAAVLDALLPKVKVQLRKRWLSHVESLKSRFPMQMPDLDDP
CCHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RSHYGLIHAVAAALDDEAVIATDVGQHQMWVAQAYPFRRARQWLTSGGLGTMGFGMPTAI
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHCCHHHHHHHHHCCCCCCCCCCCHHHH
GAALAAPERTVVCFSGDGSLQMNIQELATLAELGLNVKIVLMNNNSLGLVYQQQNLFYGK
HHHHCCCCCEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCEECH
RTFASKYRGGPDFCRIAEGYGIAAVDLDASDNPRATLAEALQAPGPCLIHASIDREQFVY
HHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEE
PMVPPGAANTEMIGG
ECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]