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The map label for this gene is nfsA [H]

Identifier: 119897244

GI number: 119897244

Start: 1024961

End: 1025785

Strand: Reverse

Name: nfsA [H]

Synonym: azo0953

Alternate gene names: 119897244

Gene position: 1025785-1024961 (Counterclockwise)

Preceding gene: 119897245

Following gene: 119897209

Centisome position: 23.44

GC content: 70.18

Gene sequence:

>825_bases
ATGAACCAGCCCGACACCCTGTTCCAGACCCGCTATGGCGACGCGGCCGCGCGCGCGCCGGCGGCCTGGTCTCCCGTCAT
CGAACACCTGCTCGGCCACCGCTCGGTCCGCGCCTACCTGCCCGACCCGGTCTCGGAAGACGAACTGGTGGCGATCGTCG
CCGCTGCACAATCGGCGGCGAGTTCGTCCAACCTGCAGGCATGGAGCGTGGTGGCGGTGCGCGACCCGGCCACGCGCGCC
GCGCTGGCGGAATGCGCAGGCGGCCAGGCCCACGTCCGCGATGCGCCGTTGCAACTGGTGTGGCTCGCCGATCTCGCCCG
CCTCGAACACCTCGCGCTTGCCAACGAAAGGCCCTCGGCGGCACTCGACTACCTCGAGATGTTCCTGGTCGGTGTCATCG
ACGCCGCGCTCGCGGCGCAGAACGCCGCCGCGGCGGCAGAATCGCTCGGCCTTGCCACCGTGTATATCGGCGGCATGCGC
AACCAGCCCGAACGCGTGGCCGAACTGCTGCGGCTGCCGTCCAAGGTCGTGGCGGTGTTCGGCATGTGCGTAGGCCGGCC
CGATCCCGCTCAGCCCGCCGAGATCAAGCCGCGCCCGCCGCAGTCGGTGGTGCTGCACCACGAAACCTACACGCCGCTCC
CCGCCCAGGACGAAGGCATCGCCGCCTACAACGGCGCGATGGCGCAGTTCTACGAGCGCCAGAACATGAAGGTGCACGGC
ACTTGGGCGGTTCACTCGGCCAAGCGCGTCGCCGGGCCGGAAAGCCTGTCGGGGCGCGACCGCTTGGTCGAAGCGCTGCA
CAACCGCGGCTTCACGCTCAAGTAG

Upstream 100 bases:

>100_bases
CCGGCCCGCGCTGGCCGCCACCACGGCGGCCGCGCGCCGCCGGCATATACTCGGGCTTCGTTGATTTTCCGGCTGCGTGC
CGCCCTTCCCCGGAGCCCCG

Downstream 100 bases:

>100_bases
CAGACGCGGGCGCAGCCGCCGCTCGCCTCAGCCCGCGCCGCGCATCGGCAGCAGGCCGCGGCAGCGCGGATAGGTACTGC
AGCCCCAGAACGCGCCGCGC

Product: putative oxygen-insentive NADPH nitroreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNQPDTLFQTRYGDAAARAPAAWSPVIEHLLGHRSVRAYLPDPVSEDELVAIVAAAQSAASSSNLQAWSVVAVRDPATRA
ALAECAGGQAHVRDAPLQLVWLADLARLEHLALANERPSAALDYLEMFLVGVIDAALAAQNAAAAAESLGLATVYIGGMR
NQPERVAELLRLPSKVVAVFGMCVGRPDPAQPAEIKPRPPQSVVLHHETYTPLPAQDEGIAAYNGAMAQFYERQNMKVHG
TWAVHSAKRVAGPESLSGRDRLVEALHNRGFTLK

Sequences:

>Translated_274_residues
MNQPDTLFQTRYGDAAARAPAAWSPVIEHLLGHRSVRAYLPDPVSEDELVAIVAAAQSAASSSNLQAWSVVAVRDPATRA
ALAECAGGQAHVRDAPLQLVWLADLARLEHLALANERPSAALDYLEMFLVGVIDAALAAQNAAAAAESLGLATVYIGGMR
NQPERVAELLRLPSKVVAVFGMCVGRPDPAQPAEIKPRPPQSVVLHHETYTPLPAQDEGIAAYNGAMAQFYERQNMKVHG
TWAVHSAKRVAGPESLSGRDRLVEALHNRGFTLK
>Mature_274_residues
MNQPDTLFQTRYGDAAARAPAAWSPVIEHLLGHRSVRAYLPDPVSEDELVAIVAAAQSAASSSNLQAWSVVAVRDPATRA
ALAECAGGQAHVRDAPLQLVWLADLARLEHLALANERPSAALDYLEMFLVGVIDAALAAQNAAAAAESLGLATVYIGGMR
NQPERVAELLRLPSKVVAVFGMCVGRPDPAQPAEIKPRPPQSVVLHHETYTPLPAQDEGIAAYNGAMAQFYERQNMKVHG
TWAVHSAKRVAGPESLSGRDRLVEALHNRGFTLK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=250, Percent_Identity=40.8, Blast_Score=158, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 29341; Mature: 29341

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPDTLFQTRYGDAAARAPAAWSPVIEHLLGHRSVRAYLPDPVSEDELVAIVAAAQSAA
CCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEHHHHHHHHC
SSSNLQAWSVVAVRDPATRAALAECAGGQAHVRDAPLQLVWLADLARLEHLALANERPSA
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHHHCCCCCHH
ALDYLEMFLVGVIDAALAAQNAAAAAESLGLATVYIGGMRNQPERVAELLRLPSKVVAVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHH
GMCVGRPDPAQPAEIKPRPPQSVVLHHETYTPLPAQDEGIAAYNGAMAQFYERQNMKVHG
HHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
TWAVHSAKRVAGPESLSGRDRLVEALHNRGFTLK
EEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNQPDTLFQTRYGDAAARAPAAWSPVIEHLLGHRSVRAYLPDPVSEDELVAIVAAAQSAA
CCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEHHHHHHHHC
SSSNLQAWSVVAVRDPATRAALAECAGGQAHVRDAPLQLVWLADLARLEHLALANERPSA
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHHHCCCCCHH
ALDYLEMFLVGVIDAALAAQNAAAAAESLGLATVYIGGMRNQPERVAELLRLPSKVVAVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHH
GMCVGRPDPAQPAEIKPRPPQSVVLHHETYTPLPAQDEGIAAYNGAMAQFYERQNMKVHG
HHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
TWAVHSAKRVAGPESLSGRDRLVEALHNRGFTLK
EEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]