Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 119897231

GI number: 119897231

Start: 1008648

End: 1009412

Strand: Direct

Name: rlmB [H]

Synonym: azo0940

Alternate gene names: 119897231

Gene position: 1008648-1009412 (Clockwise)

Preceding gene: 119897230

Following gene: 119897232

Centisome position: 23.05

GC content: 70.46

Gene sequence:

>765_bases
TTGGCTAAACCAGCAGAATCCTCGGCCACGCGCCTGATCTACGGTTTTCACGCGGTATCGGCCAAGCTCCGCCACGCGCC
CGAGGCGGTGCTGGAGGTCTATCTGTCCGGCGACCGCAAGGACGCGCGGGTCAAGAAATTCCTCGCGACCGCGGAGGCGA
TTGGCACCCGCATCGTGCCGAGCGAACACGAGCGGCTCGACGCACTGGTGGGCACGCGGCGCCATCAGGGCGTGGTGGCG
CGCATCGACGCCACCCGGCGTGAGATCAAGCTCGCCGACGTGCTTGACAACCTCGACGAGAACGCGCTGATCCTGGTCCT
CGACGGCGTGCAGGACCCGCACAACCTCGGCGCCTGCCTGCGCGTGGCAGATGCCGCCGGCGCCCACGCGGTGGTCGCGC
CCAAGGACCGCGCGGTCGGCCTCAACGCCACCGCCGCCAAGGTCGCCAGCGGTGCGGCCGACACGGTGCCCTACATCACC
GTGACCAATCTGGCGCGCGCGCTGCGCGAAATGCAGGAAGCCGGCGTGTGGGTGGTGGGCGCCGCCGGCGAGGCGGAGAA
GTCCGTCTACCAGGTGGACCAGCGCGGCCCGCTCGCATGGGTGCTCGGGGCCGAAGGCGACGGTCTGCGCCGCCTGACCC
GCGACACCTGCGACGAGCTGGCGCAGATTCCGATGCACGGCTCGGTCGAGAGTCTCAACGTGTCGGTGGCGAGCGGCATC
TGCCTGTTCGAGGCCCGCCGCCAGCGCGCCGACGTTAGCGCCTGA

Upstream 100 bases:

>100_bases
AAGGCCGCTGCGCGCAGCAGGGCCAGGGCCACCACGACAACCGAAACCGGCGACAAGGCGGAGCCCGCCAAGCCGGCAAC
AGCAAAGAAGGGTAAGCGCA

Downstream 100 bases:

>100_bases
AGCCCCGCGCGGGCGGCGGGGGGGGGGGCGCGGCCAATATGGGTGGCATGCAATCTGCATGCACACTTGGCCGCAAGGTT
ACCGTTGTCGTTCCTGGCCC

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MAKPAESSATRLIYGFHAVSAKLRHAPEAVLEVYLSGDRKDARVKKFLATAEAIGTRIVPSEHERLDALVGTRRHQGVVA
RIDATRREIKLADVLDNLDENALILVLDGVQDPHNLGACLRVADAAGAHAVVAPKDRAVGLNATAAKVASGAADTVPYIT
VTNLARALREMQEAGVWVVGAAGEAEKSVYQVDQRGPLAWVLGAEGDGLRRLTRDTCDELAQIPMHGSVESLNVSVASGI
CLFEARRQRADVSA

Sequences:

>Translated_254_residues
MAKPAESSATRLIYGFHAVSAKLRHAPEAVLEVYLSGDRKDARVKKFLATAEAIGTRIVPSEHERLDALVGTRRHQGVVA
RIDATRREIKLADVLDNLDENALILVLDGVQDPHNLGACLRVADAAGAHAVVAPKDRAVGLNATAAKVASGAADTVPYIT
VTNLARALREMQEAGVWVVGAAGEAEKSVYQVDQRGPLAWVLGAEGDGLRRLTRDTCDELAQIPMHGSVESLNVSVASGI
CLFEARRQRADVSA
>Mature_253_residues
AKPAESSATRLIYGFHAVSAKLRHAPEAVLEVYLSGDRKDARVKKFLATAEAIGTRIVPSEHERLDALVGTRRHQGVVAR
IDATRREIKLADVLDNLDENALILVLDGVQDPHNLGACLRVADAAGAHAVVAPKDRAVGLNATAAKVASGAADTVPYITV
TNLARALREMQEAGVWVVGAAGEAEKSVYQVDQRGPLAWVLGAEGDGLRRLTRDTCDELAQIPMHGSVESLNVSVASGIC
LFEARRQRADVSA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=264, Percent_Identity=28.7878787878788, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=54.320987654321, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1788935, Length=236, Percent_Identity=29.2372881355932, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=32.2368421052632, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=248, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27039; Mature: 26908

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPAESSATRLIYGFHAVSAKLRHAPEAVLEVYLSGDRKDARVKKFLATAEAIGTRIVP
CCCCCCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
SEHERLDALVGTRRHQGVVARIDATRREIKLADVLDNLDENALILVLDGVQDPHNLGACL
CHHHHHHHHHCCHHCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHH
RVADAAGAHAVVAPKDRAVGLNATAAKVASGAADTVPYITVTNLARALREMQEAGVWVVG
HHHHCCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEE
AAGEAEKSVYQVDQRGPLAWVLGAEGDGLRRLTRDTCDELAQIPMHGSVESLNVSVASGI
CCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEHHHHCH
CLFEARRQRADVSA
HHHHHHHHHCCCCC
>Mature Secondary Structure 
AKPAESSATRLIYGFHAVSAKLRHAPEAVLEVYLSGDRKDARVKKFLATAEAIGTRIVP
CCCCCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
SEHERLDALVGTRRHQGVVARIDATRREIKLADVLDNLDENALILVLDGVQDPHNLGACL
CHHHHHHHHHCCHHCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHH
RVADAAGAHAVVAPKDRAVGLNATAAKVASGAADTVPYITVTNLARALREMQEAGVWVVG
HHHHCCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEE
AAGEAEKSVYQVDQRGPLAWVLGAEGDGLRRLTRDTCDELAQIPMHGSVESLNVSVASGI
CCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEHHHHCH
CLFEARRQRADVSA
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]