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The map label for this gene is pcm1 [H]

Identifier: 119897139

GI number: 119897139

Start: 913062

End: 913715

Strand: Direct

Name: pcm1 [H]

Synonym: azo0848

Alternate gene names: 119897139

Gene position: 913062-913715 (Clockwise)

Preceding gene: 119897138

Following gene: 119897140

Centisome position: 20.87

GC content: 66.21

Gene sequence:

>654_bases
ATGAACTTCGAGAAAGCGCGCTTCAACATGGTTGAGCAACAGATCCGCCCCTGGGATGTGCTGGATCCGGCAGTGCTCGA
CCTCCTGATGACGGTCAAGCGCGAGGAATACGTGCCGGCCGCCGCCCGCGCGCTCGCGTTCGCCGACGTGGAGATTCCGC
TCGGCCAGGGCCAGGTCATGCTGAAGCCGGTCATCGAAGGCAAGATCCTGCAGTCGCTGCAACTGCACCGCTCCGACTCC
GTGCTCGAAGTGGGCGCCGGCAGCGGTTACTTCGCCGCGTTGCTTGCCGCCCGCGTCGAATGGGTGCGCACCGTCGACAT
CGAGCCCGAACTGGTCCGGTTTGCCCACGCCAACCTCGCGCGCAACGGGGTCGAGAACGTCATCGTCGAAGAGGGCGATG
CCGCCCAGGGCTGGGCGAGCCGCGCGCCCTACGACGTGATCGTGGTGTCGGGCGGGCTGCCGGTCGTGCCCCAGGCACTG
CTTGAACAACTGAAGGTGGGTGGCCGCCTGTTCGCCTTCGTGGGCGAGGCGCCGGTGATGAAGGCGCGCCTGATCACCTG
CGAGGCCGAGGGCCGCTTCCTGACCGAAGACATTTTCGAAACCCTGGTGCCGATGCTGAAGAACGCACCGCGCCAGGACA
GCTTCCGCTTCTGA

Upstream 100 bases:

>100_bases
GGCCGTTCAAGGCGCTAGAATGTGAAGGTTTTTCATTCGATTACGCTAGTATTAGATTATTCTAATTTAGCGCATGCTAA
TATTTAGGTGGGAGTCCTAG

Downstream 100 bases:

>100_bases
GGTTTTCCATGCACGAGATCACCCCCGCCCAGCTCGCCGAGTGGCTTGCCGATCCCGCCAAGATCAAACCCCTGCTGCTG
GACGTGCGCGAGCCCTGGGA

Product: putative protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2 [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNFEKARFNMVEQQIRPWDVLDPAVLDLLMTVKREEYVPAAARALAFADVEIPLGQGQVMLKPVIEGKILQSLQLHRSDS
VLEVGAGSGYFAALLAARVEWVRTVDIEPELVRFAHANLARNGVENVIVEEGDAAQGWASRAPYDVIVVSGGLPVVPQAL
LEQLKVGGRLFAFVGEAPVMKARLITCEAEGRFLTEDIFETLVPMLKNAPRQDSFRF

Sequences:

>Translated_217_residues
MNFEKARFNMVEQQIRPWDVLDPAVLDLLMTVKREEYVPAAARALAFADVEIPLGQGQVMLKPVIEGKILQSLQLHRSDS
VLEVGAGSGYFAALLAARVEWVRTVDIEPELVRFAHANLARNGVENVIVEEGDAAQGWASRAPYDVIVVSGGLPVVPQAL
LEQLKVGGRLFAFVGEAPVMKARLITCEAEGRFLTEDIFETLVPMLKNAPRQDSFRF
>Mature_217_residues
MNFEKARFNMVEQQIRPWDVLDPAVLDLLMTVKREEYVPAAARALAFADVEIPLGQGQVMLKPVIEGKILQSLQLHRSDS
VLEVGAGSGYFAALLAARVEWVRTVDIEPELVRFAHANLARNGVENVIVEEGDAAQGWASRAPYDVIVVSGGLPVVPQAL
LEQLKVGGRLFAFVGEAPVMKARLITCEAEGRFLTEDIFETLVPMLKNAPRQDSFRF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=169, Percent_Identity=31.9526627218935, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789100, Length=170, Percent_Identity=37.0588235294118, Blast_Score=106, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71983477, Length=155, Percent_Identity=32.258064516129, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193207222, Length=98, Percent_Identity=38.7755102040816, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17981723, Length=167, Percent_Identity=31.1377245508982, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23956; Mature: 23956

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFEKARFNMVEQQIRPWDVLDPAVLDLLMTVKREEYVPAAARALAFADVEIPLGQGQVM
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCEE
LKPVIEGKILQSLQLHRSDSVLEVGAGSGYFAALLAARVEWVRTVDIEPELVRFAHANLA
EHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RNGVENVIVEEGDAAQGWASRAPYDVIVVSGGLPVVPQALLEQLKVGGRLFAFVGEAPVM
HCCHHHEEEECCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCE
KARLITCEAEGRFLTEDIFETLVPMLKNAPRQDSFRF
EEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNFEKARFNMVEQQIRPWDVLDPAVLDLLMTVKREEYVPAAARALAFADVEIPLGQGQVM
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCEE
LKPVIEGKILQSLQLHRSDSVLEVGAGSGYFAALLAARVEWVRTVDIEPELVRFAHANLA
EHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RNGVENVIVEEGDAAQGWASRAPYDVIVVSGGLPVVPQALLEQLKVGGRLFAFVGEAPVM
HCCHHHEEEECCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCE
KARLITCEAEGRFLTEDIFETLVPMLKNAPRQDSFRF
EEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA