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The map label for this gene is rluF [H]

Identifier: 119897119

GI number: 119897119

Start: 886288

End: 887790

Strand: Direct

Name: rluF [H]

Synonym: azo0828

Alternate gene names: 119897119

Gene position: 886288-887790 (Clockwise)

Preceding gene: 119897118

Following gene: 119897128

Centisome position: 20.25

GC content: 75.78

Gene sequence:

>1503_bases
TTGAGCACCATCCGCAAGAATCCGCCCCAGCCGGCGCCGTCGCGCATTGCCGGTACCCTGAAGCTGCGCAAGCCTGCCGC
CGACGACAACGGCGAGTCCGCAGCCGGGGCGGATCCGGCCCAGTCCGTCGCTGCCGGCAGCGACAAGCGCGCGCGCCCGT
CATCGGCGGGCGAGGAAGGCCGCGCGCCGCGTTCGGGTGCCCGCGGCGGTCAGGCCAGCCGCGGCGAACGGCCGCGGCAG
GACGCCGAGCCGCGTCGGCAGCCGCGCGCGCGCCCGGCGCAAGAGGGCGAGGCGCCGCGGCGGTCGCGCCGCGACCCGGG
TGATGGCGGGACGGAACGGCACCCGCGGCGGGACGCCGGCGCCAGCGAAGAGCGGCCGCGTCGGCGCGAGGCGGCTGACG
AGCGTCAGGCGCGGCCCGGCTACGGCCGTCGCGACGACGGGCCGGCCCGTGCCGGTGCGCGTCCCGCGCGCGAACAGGGC
CGCGAGCGCGGCGAAGCATCCGGCCGGCGTCACGGCGGTGAGGGTGGCGAGCGGGCCTCCGCGCCGCGCGCGGCGTACGA
CGAACGCCGCCGCGCCGGAGGTGACGAGCGCGCGCCCCGCCGCGCTGCCGGCGACGCGGCCCGCACATCTCCCCGTCCCG
CCGGCCCGCGGCCGGCGGCCGCGGCGCCCCGCCCGGCCAGTGCCGAACGCGCGCCGCGCCGCCGTGAGGAGGCGCCGGCA
CGTGCCCCGGCTGCGCCGCGCGCGCCAGCCGTGCAGTCCGGCGACGGCGAGGCGCCCAGCGGCGTGCGCTTGTCCAAGGT
GATGGCCGACCGCGGCATCTGCTCGCGGCGCGAGGCCGACGAGCTGATCGAGCGCGGCTGGGTCTTCGTCGACGGTCGCC
GTGTCTCCGAACTTGGCACCCGCATCGACCCTGACGCCCGCGTCGTGCTTGCGCCCGAGGCCAAAAAGGCGCAGGCGCAG
CGCGTCACCATCCTGCTGCACAAGCCGGTGGGCTACGTTTCGGGCCAGCCGGAGCCGGGCTACGAGCCGGCGGTGACGCT
GATCGGCGGCGACAACCAGTTCGACCGCGACGTCGCGCAGCGCTTCCACCCCTCGCAGCTGAAGGGGCTGGCGCCGGCGG
GGCGACTGGACATCGACTCCACCGGTCTGCTGGTGCTGACCCAGGACGGCCGCATCGCCCGCCAGCTGATCGGCGACGAC
TCGAAGGTCGAGAAGGAATACCTGGTGCGCGTCGAGGGCGAGCTGGTCGACGACGGCCTTGCGCTGCTCAATCACGGCCT
GTCGCTCGACGACAAGCCGCTGCGCCCGGCCAAGGTGGAGTGGATCAACGAAGACCAGCTGCGCTTCGTGTTGAAAGAGG
GCCGCAAGCGCCAGATCCGCCGCATGTGCGAACTGGTCGGCCTCCGCGTGGTGGGCCTGAAGCGGGTGCGCATCGGCCGC
GTCAAGCTCGGCGACCTGCCGCTGGGCGAGTGGCGCTATCTGCGCGACGACGAGGTGTTCTGA

Upstream 100 bases:

>100_bases
TCTGGCCCTGTCGGGCCGCCGGAACTCGCCGGGGGCTGGGGCGCTGGCGCATAATCGCGCCTGTTTCCGCATCGCGCCGT
CCGGCGCACTGAGGTCCCCT

Downstream 100 bases:

>100_bases
CGCCGCGCCGGCGTGCCGGCGTGAAGCGGCTGCATCGACGACGGGGCCTGCGGGCCCCGTCCTGCGTTTACCGGGCGATC
ACCGCGGGCGGCCGCTTCAG

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSTIRKNPPQPAPSRIAGTLKLRKPAADDNGESAAGADPAQSVAAGSDKRARPSSAGEEGRAPRSGARGGQASRGERPRQ
DAEPRRQPRARPAQEGEAPRRSRRDPGDGGTERHPRRDAGASEERPRRREAADERQARPGYGRRDDGPARAGARPAREQG
RERGEASGRRHGGEGGERASAPRAAYDERRRAGGDERAPRRAAGDAARTSPRPAGPRPAAAAPRPASAERAPRRREEAPA
RAPAAPRAPAVQSGDGEAPSGVRLSKVMADRGICSRREADELIERGWVFVDGRRVSELGTRIDPDARVVLAPEAKKAQAQ
RVTILLHKPVGYVSGQPEPGYEPAVTLIGGDNQFDRDVAQRFHPSQLKGLAPAGRLDIDSTGLLVLTQDGRIARQLIGDD
SKVEKEYLVRVEGELVDDGLALLNHGLSLDDKPLRPAKVEWINEDQLRFVLKEGRKRQIRRMCELVGLRVVGLKRVRIGR
VKLGDLPLGEWRYLRDDEVF

Sequences:

>Translated_500_residues
MSTIRKNPPQPAPSRIAGTLKLRKPAADDNGESAAGADPAQSVAAGSDKRARPSSAGEEGRAPRSGARGGQASRGERPRQ
DAEPRRQPRARPAQEGEAPRRSRRDPGDGGTERHPRRDAGASEERPRRREAADERQARPGYGRRDDGPARAGARPAREQG
RERGEASGRRHGGEGGERASAPRAAYDERRRAGGDERAPRRAAGDAARTSPRPAGPRPAAAAPRPASAERAPRRREEAPA
RAPAAPRAPAVQSGDGEAPSGVRLSKVMADRGICSRREADELIERGWVFVDGRRVSELGTRIDPDARVVLAPEAKKAQAQ
RVTILLHKPVGYVSGQPEPGYEPAVTLIGGDNQFDRDVAQRFHPSQLKGLAPAGRLDIDSTGLLVLTQDGRIARQLIGDD
SKVEKEYLVRVEGELVDDGLALLNHGLSLDDKPLRPAKVEWINEDQLRFVLKEGRKRQIRRMCELVGLRVVGLKRVRIGR
VKLGDLPLGEWRYLRDDEVF
>Mature_499_residues
STIRKNPPQPAPSRIAGTLKLRKPAADDNGESAAGADPAQSVAAGSDKRARPSSAGEEGRAPRSGARGGQASRGERPRQD
AEPRRQPRARPAQEGEAPRRSRRDPGDGGTERHPRRDAGASEERPRRREAADERQARPGYGRRDDGPARAGARPAREQGR
ERGEASGRRHGGEGGERASAPRAAYDERRRAGGDERAPRRAAGDAARTSPRPAGPRPAAAAPRPASAERAPRRREEAPAR
APAAPRAPAVQSGDGEAPSGVRLSKVMADRGICSRREADELIERGWVFVDGRRVSELGTRIDPDARVVLAPEAKKAQAQR
VTILLHKPVGYVSGQPEPGYEPAVTLIGGDNQFDRDVAQRFHPSQLKGLAPAGRLDIDSTGLLVLTQDGRIARQLIGDDS
KVEKEYLVRVEGELVDDGLALLNHGLSLDDKPLRPAKVEWINEDQLRFVLKEGRKRQIRRMCELVGLRVVGLKRVRIGRV
KLGDLPLGEWRYLRDDEVF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=246, Percent_Identity=38.2113821138211, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788510, Length=241, Percent_Identity=34.4398340248963, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=34.412955465587, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081838, Length=160, Percent_Identity=36.875, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 54374; Mature: 54243

Theoretical pI: Translated: 11.16; Mature: 11.16

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIRKNPPQPAPSRIAGTLKLRKPAADDNGESAAGADPAQSVAAGSDKRARPSSAGEEG
CCCCCCCCCCCCHHHHEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCC
RAPRSGARGGQASRGERPRQDAEPRRQPRARPAQEGEAPRRSRRDPGDGGTERHPRRDAG
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCC
ASEERPRRREAADERQARPGYGRRDDGPARAGARPAREQGRERGEASGRRHGGEGGERAS
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
APRAAYDERRRAGGDERAPRRAAGDAARTSPRPAGPRPAAAAPRPASAERAPRRREEAPA
CCHHHHHHHHHCCCCCCCCHHHCCCHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCC
RAPAAPRAPAVQSGDGEAPSGVRLSKVMADRGICSRREADELIERGWVFVDGRRVSELGT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCHHHHHCC
RIDPDARVVLAPEAKKAQAQRVTILLHKPVGYVSGQPEPGYEPAVTLIGGDNQFDRDVAQ
CCCCCCEEEECCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHH
RFHPSQLKGLAPAGRLDIDSTGLLVLTQDGRIARQLIGDDSKVEKEYLVRVEGELVDDGL
HCCHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHEEECCHHHHHHH
ALLNHGLSLDDKPLRPAKVEWINEDQLRFVLKEGRKRQIRRMCELVGLRVVGLKRVRIGR
HHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECE
VKLGDLPLGEWRYLRDDEVF
EEECCCCCCCCEECCCCCCC
>Mature Secondary Structure 
STIRKNPPQPAPSRIAGTLKLRKPAADDNGESAAGADPAQSVAAGSDKRARPSSAGEEG
CCCCCCCCCCCHHHHEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCC
RAPRSGARGGQASRGERPRQDAEPRRQPRARPAQEGEAPRRSRRDPGDGGTERHPRRDAG
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCC
ASEERPRRREAADERQARPGYGRRDDGPARAGARPAREQGRERGEASGRRHGGEGGERAS
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
APRAAYDERRRAGGDERAPRRAAGDAARTSPRPAGPRPAAAAPRPASAERAPRRREEAPA
CCHHHHHHHHHCCCCCCCCHHHCCCHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCC
RAPAAPRAPAVQSGDGEAPSGVRLSKVMADRGICSRREADELIERGWVFVDGRRVSELGT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCHHHHHCC
RIDPDARVVLAPEAKKAQAQRVTILLHKPVGYVSGQPEPGYEPAVTLIGGDNQFDRDVAQ
CCCCCCEEEECCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHH
RFHPSQLKGLAPAGRLDIDSTGLLVLTQDGRIARQLIGDDSKVEKEYLVRVEGELVDDGL
HCCHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHEEECCHHHHHHH
ALLNHGLSLDDKPLRPAKVEWINEDQLRFVLKEGRKRQIRRMCELVGLRVVGLKRVRIGR
HHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECE
VKLGDLPLGEWRYLRDDEVF
EEECCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]