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The map label for this gene is vacJ [H]

Identifier: 119897100

GI number: 119897100

Start: 866632

End: 867492

Strand: Direct

Name: vacJ [H]

Synonym: azo0809

Alternate gene names: 119897100

Gene position: 866632-867492 (Clockwise)

Preceding gene: 119897099

Following gene: 119897101

Centisome position: 19.8

GC content: 67.02

Gene sequence:

>861_bases
ATGTCGTACCGGTTCCGCTCCGTTGCCGGCAGTCGCGGCCTGCGGGTCGTGCTGGCGGCCCTGCTTGCCGGTGGCTGCGC
CACGGCCACGACCCATCCCCAGGATCCGCTCGAAAACTACAACCGGGCAATGTTCGCGTTCAACGACCGCGTCGACCGCG
CGGTGATCAAGCCGGTGGCGGAGGCTTACGAGACCGTGGTGCCGTCCCCCGTGCGCAGCGGCGTCGGCAACGTTTTCGGC
AACCTCGGCGATCCCTGGATCGCGATCAACAACCTGCTGCAGGGCAAGTTGCTTGATGCGCTGTCGGATGGCGCCCGCTT
CGTCATGAACACCACCGTCGGCGTGCTGGGAATCCTCGATGTCGCGACCGCGGCCGGCCTCACCAAGCATGACGAGGACT
TCGGCCAGACGCTGGGCGCCTGGGGCGTGGGCGAGGGTGCCTATCTGGTGCTGCCCTTCTTCGGTCCGCGCACGGTGCGC
GACGCGGCCGCCTTGCCGCTGGATGTGATGGCCGACGACGTGTGGGCGATCCAGCACGTGCCGACGCGCAACAGCATGAC
GGCGCTGCGCCTGGTGCACACGCGCTCGACCCTGCTCGGCACCGAAAAGACGCTGGAAGAGGGCACCATCGACAAGTACG
CCTACTCGCGCGACTTCTACCTGGAGCAGCGGCGGTACAAGGTGTTCGATGGCAATCCGCCGCGCAAGTACGAAGACTTC
GACGACGAGGGTGCCGCATTGCCGCAGCCCACGCAGGCGGATTTCGTCGCCCGCGCCGCGGTCGAAAGTCTTGAAGTGCT
GGCGGTCGGGGGCGAGTTCGCCCAGGCCCTACCCCCCAACAACGAGGAATCGAATCCGTGA

Upstream 100 bases:

>100_bases
AAGCTGATCGGTCAGTTCCTGTTCAACAAGGCGGCCGAACCCGCCGCGCAGCCGCAATAACAACTACCGAGAAATCCGAC
CTATGCCACGGAGAGCATGC

Downstream 100 bases:

>100_bases
ATGCGATGAGAAGCTGGCTCTCCCTGTGCCTGCGGCTGGCGCTGCTCGCGGCCGTGTTGTCGGCCCCGACGGCGCGCGCG
GCGGACCCGATGGGCCCCGA

Product: putative surface lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSYRFRSVAGSRGLRVVLAALLAGGCATATTHPQDPLENYNRAMFAFNDRVDRAVIKPVAEAYETVVPSPVRSGVGNVFG
NLGDPWIAINNLLQGKLLDALSDGARFVMNTTVGVLGILDVATAAGLTKHDEDFGQTLGAWGVGEGAYLVLPFFGPRTVR
DAAALPLDVMADDVWAIQHVPTRNSMTALRLVHTRSTLLGTEKTLEEGTIDKYAYSRDFYLEQRRYKVFDGNPPRKYEDF
DDEGAALPQPTQADFVARAAVESLEVLAVGGEFAQALPPNNEESNP

Sequences:

>Translated_286_residues
MSYRFRSVAGSRGLRVVLAALLAGGCATATTHPQDPLENYNRAMFAFNDRVDRAVIKPVAEAYETVVPSPVRSGVGNVFG
NLGDPWIAINNLLQGKLLDALSDGARFVMNTTVGVLGILDVATAAGLTKHDEDFGQTLGAWGVGEGAYLVLPFFGPRTVR
DAAALPLDVMADDVWAIQHVPTRNSMTALRLVHTRSTLLGTEKTLEEGTIDKYAYSRDFYLEQRRYKVFDGNPPRKYEDF
DDEGAALPQPTQADFVARAAVESLEVLAVGGEFAQALPPNNEESNP
>Mature_285_residues
SYRFRSVAGSRGLRVVLAALLAGGCATATTHPQDPLENYNRAMFAFNDRVDRAVIKPVAEAYETVVPSPVRSGVGNVFGN
LGDPWIAINNLLQGKLLDALSDGARFVMNTTVGVLGILDVATAAGLTKHDEDFGQTLGAWGVGEGAYLVLPFFGPRTVRD
AAALPLDVMADDVWAIQHVPTRNSMTALRLVHTRSTLLGTEKTLEEGTIDKYAYSRDFYLEQRRYKVFDGNPPRKYEDFD
DEGAALPQPTQADFVARAAVESLEVLAVGGEFAQALPPNNEESNP

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=228, Percent_Identity=34.6491228070175, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 30987; Mature: 30856

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYRFRSVAGSRGLRVVLAALLAGGCATATTHPQDPLENYNRAMFAFNDRVDRAVIKPVA
CCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCEEEEECCHHHHHHHHHHH
EAYETVVPSPVRSGVGNVFGNLGDPWIAINNLLQGKLLDALSDGARFVMNTTVGVLGILD
HHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
VATAAGLTKHDEDFGQTLGAWGVGEGAYLVLPFFGPRTVRDAAALPLDVMADDVWAIQHV
HHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHCCCHHHHHCCHHHEEEC
PTRNSMTALRLVHTRSTLLGTEKTLEEGTIDKYAYSRDFYLEQRRYKVFDGNPPRKYEDF
CCCCCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCHHHHHCCCEEEECCCCCCCCCCC
DDEGAALPQPTQADFVARAAVESLEVLAVGGEFAQALPPNNEESNP
CCCCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SYRFRSVAGSRGLRVVLAALLAGGCATATTHPQDPLENYNRAMFAFNDRVDRAVIKPVA
CCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCEEEEECCHHHHHHHHHHH
EAYETVVPSPVRSGVGNVFGNLGDPWIAINNLLQGKLLDALSDGARFVMNTTVGVLGILD
HHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
VATAAGLTKHDEDFGQTLGAWGVGEGAYLVLPFFGPRTVRDAAALPLDVMADDVWAIQHV
HHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHCCCHHHHHCCHHHEEEC
PTRNSMTALRLVHTRSTLLGTEKTLEEGTIDKYAYSRDFYLEQRRYKVFDGNPPRKYEDF
CCCCCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCHHHHHCCCEEEECCCCCCCCCCC
DDEGAALPQPTQADFVARAAVESLEVLAVGGEFAQALPPNNEESNP
CCCCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]