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The map label for this gene is mlaE [H]

Identifier: 119897098

GI number: 119897098

Start: 865327

End: 866118

Strand: Direct

Name: mlaE [H]

Synonym: azo0807

Alternate gene names: 119897098

Gene position: 865327-866118 (Clockwise)

Preceding gene: 119897097

Following gene: 119897099

Centisome position: 19.77

GC content: 67.3

Gene sequence:

>792_bases
ATGAGCGGGCTGGTCGCGGCGCTCCGGCGCCTGGGCGGGATGAGCATCGACGCGGTGTGGCGGCTGGGCTTTGCCACCCG
CTTCCTGTTGATGGTGCTGGTGTATTCCGGGCAGTCGGTGCGCCGCATTCACCTTACGCTGCGCGAGATCTACTTCGCGG
GCGTGCTGTCGCTGCTGATCATCCTCGTGTCGGGGCTGTTCGTGGGGCTGGTGCTCGGGCTGCAGGGCTATGAAACCTTG
CAGCGTTTCGGGTCCAGCGATGCGCTCGGCGTGCTGGTCGCGCTGTCGCTGACGCGCGAACTCGGCCCGGTCGTGGCGGC
GCTGCTCTTCGCCAGCCGCGCGGGGTCGGCAGTCACCGCGGAGATCGGCCTGATGAAGGCCACCGAGCAGCTCAAGGCGA
TGGACATGATGGCGGTGAATCCGCTGGCGCGGGTGGTAGCGCCGCGGTTCTGGGGCGGGGTGATCTCGATGCCGCTGCTC
GCCGCGATGTTCTCCGCGATGGGCGTGTTCGGCGGCTGGCTCATCGGCGTCGTCTTCATCGGTGTGGACGACGGCGCGTT
CTGGTCGCAGATGCAGGCCGCGGTGGATGTGCGCTATGACGTGCTGAACGGCGTCATCAAGTCCTTCGTGTTCGGCGTCG
CGGTGTCCTTGATCGCGGTGTTCGAGGGCTACGACTGCACCCCCACCGCCGAGGGCGTCTCGCGGGCGATCACCCGCACC
GTGGTCAGTTCGGCGCTCGCCATCCTGGCGCTCGACTTCGTGCTCACCTCTTTCATGTTCCGGGGACAATGA

Upstream 100 bases:

>100_bases
ACCCCTTCGTGCATCAATTCGTGCATGCCGAGGCGGACGGCCCGGTGCCGTTCCACTACCCGGCGCCCCCGGTCGCCTCG
CTGATCGAGGAGACACCGCG

Downstream 100 bases:

>100_bases
TGAGTCGTACCACGCTCGACCTCTGGGTCGGCATCTTCGTTGCGCTGGGTCTGGCGGCGCTGGTCTTTCTGGCGATGAAG
GTCGGCAACTTTTCCGGCCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSGLVAALRRLGGMSIDAVWRLGFATRFLLMVLVYSGQSVRRIHLTLREIYFAGVLSLLIILVSGLFVGLVLGLQGYETL
QRFGSSDALGVLVALSLTRELGPVVAALLFASRAGSAVTAEIGLMKATEQLKAMDMMAVNPLARVVAPRFWGGVISMPLL
AAMFSAMGVFGGWLIGVVFIGVDDGAFWSQMQAAVDVRYDVLNGVIKSFVFGVAVSLIAVFEGYDCTPTAEGVSRAITRT
VVSSALAILALDFVLTSFMFRGQ

Sequences:

>Translated_263_residues
MSGLVAALRRLGGMSIDAVWRLGFATRFLLMVLVYSGQSVRRIHLTLREIYFAGVLSLLIILVSGLFVGLVLGLQGYETL
QRFGSSDALGVLVALSLTRELGPVVAALLFASRAGSAVTAEIGLMKATEQLKAMDMMAVNPLARVVAPRFWGGVISMPLL
AAMFSAMGVFGGWLIGVVFIGVDDGAFWSQMQAAVDVRYDVLNGVIKSFVFGVAVSLIAVFEGYDCTPTAEGVSRAITRT
VVSSALAILALDFVLTSFMFRGQ
>Mature_262_residues
SGLVAALRRLGGMSIDAVWRLGFATRFLLMVLVYSGQSVRRIHLTLREIYFAGVLSLLIILVSGLFVGLVLGLQGYETLQ
RFGSSDALGVLVALSLTRELGPVVAALLFASRAGSAVTAEIGLMKATEQLKAMDMMAVNPLARVVAPRFWGGVISMPLLA
AMFSAMGVFGGWLIGVVFIGVDDGAFWSQMQAAVDVRYDVLNGVIKSFVFGVAVSLIAVFEGYDCTPTAEGVSRAITRTV
VSSALAILALDFVLTSFMFRGQ

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=257, Percent_Identity=57.5875486381323, Blast_Score=281, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28013; Mature: 27882

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGLVAALRRLGGMSIDAVWRLGFATRFLLMVLVYSGQSVRRIHLTLREIYFAGVLSLLI
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ILVSGLFVGLVLGLQGYETLQRFGSSDALGVLVALSLTRELGPVVAALLFASRAGSAVTA
HHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EIGLMKATEQLKAMDMMAVNPLARVVAPRFWGGVISMPLLAAMFSAMGVFGGWLIGVVFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVDDGAFWSQMQAAVDVRYDVLNGVIKSFVFGVAVSLIAVFEGYDCTPTAEGVSRAITRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VVSSALAILALDFVLTSFMFRGQ
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]