BacMap

Definition	Azoarcus sp. BH72 chromosome, complete genome.
Accession	NC_008702
Length	4,376,040

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The map label for this gene is paaF1 [H]

Identifier: 119897081

GI number: 119897081

Start: 848399

End: 849175

Strand: Reverse

Name: paaF1 [H]

Synonym: azo0790

Alternate gene names: 119897081

Gene position: 849175-848399 (Counterclockwise)

Preceding gene: 119897082

Following gene: 119897080

Centisome position: 19.41

GC content: 65.77

Gene sequence:

>777_bases
ATGAGCTTCGAGAACATCCTCACCGAGACCCGCGGCCGCGTCGGGCTCATCACCCTCAACCGGCCGAAGGCGCTCAACGC
GCTCAATGACGCCCTGGTGGACGAAGTCGGCCAGGCGCTGGACGCCTTCGAGGCCGACGAGAACATCGGCGCAATCGTCA
TCACCGGTTCGGAGAAGGCCTTCGCCGCGGGCGCCGACATCGGCGCCATGGCGACCTTCTCGTACATGGACGCCTACAAG
GGCGACTACATCACCCGCAACTGGGAACGCGTGAAGACCTGCCGCAAGCCGGTGATCGCCGCGGTGGCCGGTTTCGCCCT
CGGCGGCGGCTGCGAACTGGCGATGATGTGCGACATCATCATCGCCGCCGACACCGCGAAGTTCGGACAACCCGAAGTCA
AGCTCGGCATCCTGCCGGGTGCCGGCGGAACCCAGCGCCTCCCGCGCGCAGTGGGCAAGGCCAAGGCGATGGACATGTGC
CTGACCGCCCGCTTCATGGACGCCACCGAAGCGGAACGCGCCGGCCTGGTGTCTCGCGTCGTCCCGGCCGACACGCTCGT
CGATGAGGCGCTCTCGGCGGCTGAAGCCATCGCCAGCTACTCGCTGCCGGTCGTCATGATGATCAAGGAATCGGTCAACC
GGGCATTCGAAAGCAGCCTCAACGAAGGCCTGCTGTTCGAGCGCCGCGTGTTCCACGCCGCCTTCGCGCTCAATGACCAG
AAAGAAGGCATGGCGGCCTTCGTCGCCAAGCGCAAAGCCAACTTCAGTCACGACTGA

Upstream 100 bases:

>100_bases
ACGGCCGGGTGCGCGCGGTGATCCAGCCCAAGAGTGACAACAAAGCGGGCGGCGCCGAAAGCGCCAAGCCCTGAAAACCA
CGCACTGCCCAGGAGATAGC

Downstream 100 bases:

>100_bases
GCGGCCGCCCACAGCCCGCCTCCCGCGGCGGGGCGCTCGCCCCGCCCGCCTCGCCGCTGTTGCCCACTCCGGGCACTTCC
GACTGCGGAAACTCCCTGCA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSFENILTETRGRVGLITLNRPKALNALNDALVDEVGQALDAFEADENIGAIVITGSEKAFAAGADIGAMATFSYMDAYK
GDYITRNWERVKTCRKPVIAAVAGFALGGGCELAMMCDIIIAADTAKFGQPEVKLGILPGAGGTQRLPRAVGKAKAMDMC
LTARFMDATEAERAGLVSRVVPADTLVDEALSAAEAIASYSLPVVMMIKESVNRAFESSLNEGLLFERRVFHAAFALNDQ
KEGMAAFVAKRKANFSHD

Sequences:

>Translated_258_residues
MSFENILTETRGRVGLITLNRPKALNALNDALVDEVGQALDAFEADENIGAIVITGSEKAFAAGADIGAMATFSYMDAYK
GDYITRNWERVKTCRKPVIAAVAGFALGGGCELAMMCDIIIAADTAKFGQPEVKLGILPGAGGTQRLPRAVGKAKAMDMC
LTARFMDATEAERAGLVSRVVPADTLVDEALSAAEAIASYSLPVVMMIKESVNRAFESSLNEGLLFERRVFHAAFALNDQ
KEGMAAFVAKRKANFSHD
>Mature_257_residues
SFENILTETRGRVGLITLNRPKALNALNDALVDEVGQALDAFEADENIGAIVITGSEKAFAAGADIGAMATFSYMDAYKG
DYITRNWERVKTCRKPVIAAVAGFALGGGCELAMMCDIIIAADTAKFGQPEVKLGILPGAGGTQRLPRAVGKAKAMDMCL
TARFMDATEAERAGLVSRVVPADTLVDEALSAAEAIASYSLPVVMMIKESVNRAFESSLNEGLLFERRVFHAAFALNDQK
EGMAAFVAKRKANFSHD

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=57.9766536964981, Blast_Score=298, Evalue=2e-81,
Organism=Homo sapiens, GI68989263, Length=234, Percent_Identity=36.7521367521368, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=34.5864661654135, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=32.4110671936759, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI70995211, Length=240, Percent_Identity=35, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=37.8531073446328, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=30.6603773584906, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI37594471, Length=212, Percent_Identity=30.6603773584906, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=32.3770491803279, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI25453481, Length=211, Percent_Identity=26.5402843601896, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI51538573, Length=211, Percent_Identity=26.5402843601896, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=26.5402843601896, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=26.5402843601896, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=24.7863247863248, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI157694516, Length=234, Percent_Identity=24.7863247863248, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI45643119, Length=225, Percent_Identity=28.4444444444444, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260275230, Length=225, Percent_Identity=28.4444444444444, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4757968, Length=260, Percent_Identity=25, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI49355787, Length=260, Percent_Identity=25, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260274832, Length=225, Percent_Identity=28.4444444444444, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI261878539, Length=133, Percent_Identity=37.593984962406, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221307494, Length=188, Percent_Identity=30.8510638297872, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=188, Percent_Identity=30.8510638297872, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136753, Length=188, Percent_Identity=30.8510638297872, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI116235487, Length=203, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI157694520, Length=197, Percent_Identity=25.3807106598985, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=43.6, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=36.3636363636364, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788597, Length=270, Percent_Identity=34.4444444444444, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=35.1351351351351, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=32.0754716981132, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788682, Length=224, Percent_Identity=32.5892857142857, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790281, Length=178, Percent_Identity=32.5842696629214, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=55.1020408163265, Blast_Score=273, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=51.440329218107, Blast_Score=250, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17540306, Length=239, Percent_Identity=34.7280334728033, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=32.2727272727273, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=257, Percent_Identity=31.9066147859922, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=199, Percent_Identity=34.6733668341709, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=201, Percent_Identity=37.3134328358209, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=201, Percent_Identity=37.3134328358209, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=201, Percent_Identity=37.3134328358209, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=29.3333333333333, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=272, Percent_Identity=29.7794117647059, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=30.952380952381, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=193, Percent_Identity=31.6062176165803, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=193, Percent_Identity=31.6062176165803, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=244, Percent_Identity=29.9180327868852, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=201, Percent_Identity=29.8507462686567, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=184, Percent_Identity=29.3478260869565, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=56.9230769230769, Blast_Score=299, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=56.9230769230769, Blast_Score=299, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=33.976833976834, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19920382, Length=239, Percent_Identity=33.8912133891213, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21357171, Length=259, Percent_Identity=28.957528957529, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=32.3943661971831, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=26.8774703557312, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=29.9465240641711, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571729, Length=242, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571730, Length=242, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921000, Length=193, Percent_Identity=35.2331606217617, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583077, Length=193, Percent_Identity=35.2331606217617, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583079, Length=193, Percent_Identity=35.2331606217617, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27535; Mature: 27404

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFENILTETRGRVGLITLNRPKALNALNDALVDEVGQALDAFEADENIGAIVITGSEKA
CCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
FAAGADIGAMATFSYMDAYKGDYITRNWERVKTCRKPVIAAVAGFALGGGCELAMMCDII
EECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IAADTAKFGQPEVKLGILPGAGGTQRLPRAVGKAKAMDMCLTARFMDATEAERAGLVSRV
HEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPADTLVDEALSAAEAIASYSLPVVMMIKESVNRAFESSLNEGLLFERRVFHAAFALNDQ
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
KEGMAAFVAKRKANFSHD
CCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SFENILTETRGRVGLITLNRPKALNALNDALVDEVGQALDAFEADENIGAIVITGSEKA
CHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
FAAGADIGAMATFSYMDAYKGDYITRNWERVKTCRKPVIAAVAGFALGGGCELAMMCDII
EECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IAADTAKFGQPEVKLGILPGAGGTQRLPRAVGKAKAMDMCLTARFMDATEAERAGLVSRV
HEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPADTLVDEALSAAEAIASYSLPVVMMIKESVNRAFESSLNEGLLFERRVFHAAFALNDQ
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
KEGMAAFVAKRKANFSHD
CCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]