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The map label for this gene is miaB

Identifier: 119897069

GI number: 119897069

Start: 833091

End: 834440

Strand: Direct

Name: miaB

Synonym: azo0778

Alternate gene names: 119897069

Gene position: 833091-834440 (Clockwise)

Preceding gene: 119897068

Following gene: 119897070

Centisome position: 19.04

GC content: 65.26

Gene sequence:

>1350_bases
ATGAAGAAGCTCTACATCCGCACCTTCGGGTGCCAGATGAACGAGTACGACTCCGAAAAGATGGCGGACGTGCTCGGTGC
CGGCGAAGGCATTGCCAAGACGGACAACCCGGAGGAGGCGGACGTCATCCTCTTCAACACCTGCTCCGTGCGCGAGAAGG
CGCAGGAAAAGGTGTTCCACGACCTCGGGCGAGTCAAGCACCTGAAGCAGCTCAAGCCCGATCTCATCATCGGTGTCGGT
GGCTGTGTAGCGAGCCAGGAGGGCGATGCCATCGTCGCCCGCGCGCCTTATGTGGATGTGGTCTTTGGGCCGCAAACCCT
GCACCGGCTGCCGGCGCTGATCGCGGCGCGCAAGCAGAGCGGCCGCTCGCAGGTGGATATCTCGTTTCCCGAGATCGAGA
AGTTCGACGCCATGCCGCCCGCGCGGGTCGAAGGGGCGAGCGCCTTTGTGTCGATCATGGAAGGCTGTTCCAAGTACTGC
ACCTTCTGCGTCGTGCCTTATACCCGCGGCGAGGAGATCTCGCGCCCGCTCGAAGACGTGCTGGCGGAGATTGCCGGCTT
GGCGGAGCAGGGCGTCAAGGAAGTGACGCTGCTCGGCCAGAACGTCAACGCCTGGCGCGGCGAGATCGTGCGTGACGCGG
GCGAGGAGGGCGACTTTGCCTTCCTGCTCGAATGCGTCGCGGAGATTCCCGGCATCGAGCGCATCCGCTACACGACCTCG
CACCCGCGCGAGATGACGCAGCGCTTGTTCGACGCCTACGTGAAGATCCCCAAGCTGGTGTCGCAGCTGCATCTGCCGGT
CCAGTCGGGCTCCGACCGCATCCTGGCGGCGATGAAGCGCGGCTACTCGGTGCTCGAGTTCAAGTCCATCGTGCGCAAGC
TGCGCGCGGCGCGCCCCGACCTGTCGCTGTCGTCCGACTTCATCGTAGGCTTCCCCGGCGAAACCGAGGAAGATTTCGAG
AAGACGATGAAGCTGATCGACGAGGTCGGCTTCGACGGCTCCTTCAGCTTTGTCTACAGCGCCCGACCCGGCACGCCCGC
CGCGGACCTCGAAGATCCGGTGCCGCAGGAGACCAAGCTGGCGTGGCTTGCCCGGCTGCAAAAGCGCATCGACGAACAGG
CCCAGGCGGTCAGCCAGTCGATGGTGGGCAGCGCGCAGCGCATCCTGGTCGAGGGCGTCTCGCGCAAGAGCGCGGAGGAA
CTGATGGGGCGCACCGACAACAATCGCGTGGTCAACTTCCCGGCGCCGTCGCCCCACCGCGACCGGCTCGTCGGCCAGTT
CATCGAGGTGCGGATCACCTCGGCGCTGCCGCACAGCCTGCGCGGCGAAATCCTTACCCGGGAAAGCTGA

Upstream 100 bases:

>100_bases
GGGCGCAAATCGACAACGCGGGCCAGGTGCACCGGGTGTGGGTGCTTACCCCGGCGGAAATCGCGGTGGTCGACCCCAAG
CAGTAAACGGAATTTCCCCG

Downstream 100 bases:

>100_bases
TGGCCAAGATTCTGGAAGTGTTCTTCGAGCCGGTGGATAACCTCCGGCTGGCGCGCTTGTGCGGGGTGCTGGACGAGAAC
CTGCGCCAGATCGAGAACGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKKLYIRTFGCQMNEYDSEKMADVLGAGEGIAKTDNPEEADVILFNTCSVREKAQEKVFHDLGRVKHLKQLKPDLIIGVG
GCVASQEGDAIVARAPYVDVVFGPQTLHRLPALIAARKQSGRSQVDISFPEIEKFDAMPPARVEGASAFVSIMEGCSKYC
TFCVVPYTRGEEISRPLEDVLAEIAGLAEQGVKEVTLLGQNVNAWRGEIVRDAGEEGDFAFLLECVAEIPGIERIRYTTS
HPREMTQRLFDAYVKIPKLVSQLHLPVQSGSDRILAAMKRGYSVLEFKSIVRKLRAARPDLSLSSDFIVGFPGETEEDFE
KTMKLIDEVGFDGSFSFVYSARPGTPAADLEDPVPQETKLAWLARLQKRIDEQAQAVSQSMVGSAQRILVEGVSRKSAEE
LMGRTDNNRVVNFPAPSPHRDRLVGQFIEVRITSALPHSLRGEILTRES

Sequences:

>Translated_449_residues
MKKLYIRTFGCQMNEYDSEKMADVLGAGEGIAKTDNPEEADVILFNTCSVREKAQEKVFHDLGRVKHLKQLKPDLIIGVG
GCVASQEGDAIVARAPYVDVVFGPQTLHRLPALIAARKQSGRSQVDISFPEIEKFDAMPPARVEGASAFVSIMEGCSKYC
TFCVVPYTRGEEISRPLEDVLAEIAGLAEQGVKEVTLLGQNVNAWRGEIVRDAGEEGDFAFLLECVAEIPGIERIRYTTS
HPREMTQRLFDAYVKIPKLVSQLHLPVQSGSDRILAAMKRGYSVLEFKSIVRKLRAARPDLSLSSDFIVGFPGETEEDFE
KTMKLIDEVGFDGSFSFVYSARPGTPAADLEDPVPQETKLAWLARLQKRIDEQAQAVSQSMVGSAQRILVEGVSRKSAEE
LMGRTDNNRVVNFPAPSPHRDRLVGQFIEVRITSALPHSLRGEILTRES
>Mature_449_residues
MKKLYIRTFGCQMNEYDSEKMADVLGAGEGIAKTDNPEEADVILFNTCSVREKAQEKVFHDLGRVKHLKQLKPDLIIGVG
GCVASQEGDAIVARAPYVDVVFGPQTLHRLPALIAARKQSGRSQVDISFPEIEKFDAMPPARVEGASAFVSIMEGCSKYC
TFCVVPYTRGEEISRPLEDVLAEIAGLAEQGVKEVTLLGQNVNAWRGEIVRDAGEEGDFAFLLECVAEIPGIERIRYTTS
HPREMTQRLFDAYVKIPKLVSQLHLPVQSGSDRILAAMKRGYSVLEFKSIVRKLRAARPDLSLSSDFIVGFPGETEEDFE
KTMKLIDEVGFDGSFSFVYSARPGTPAADLEDPVPQETKLAWLARLQKRIDEQAQAVSQSMVGSAQRILVEGVSRKSAEE
LMGRTDNNRVVNFPAPSPHRDRLVGQFIEVRITSALPHSLRGEILTRES

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=39.8340248962656, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=39.8340248962656, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI93277076, Length=462, Percent_Identity=28.1385281385281, Blast_Score=166, Evalue=6e-41,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=65.1006711409396, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1787057, Length=416, Percent_Identity=27.6442307692308, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=34.8936170212766, Blast_Score=268, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI71996771, Length=312, Percent_Identity=30.7692307692308, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21356207, Length=484, Percent_Identity=36.7768595041322, Blast_Score=297, Evalue=8e-81,
Organism=Drosophila melanogaster, GI19922432, Length=463, Percent_Identity=28.9416846652268, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_AZOSB (A1K3J0)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932282.1
- ProteinModelPortal:   A1K3J0
- SMR:   A1K3J0
- STRING:   A1K3J0
- GeneID:   4606561
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0778
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   A1K3J0
- ProtClustDB:   PRK14325
- BioCyc:   ASP62928:AZO0778-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49687; Mature: 49687

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLYIRTFGCQMNEYDSEKMADVLGAGEGIAKTDNPEEADVILFNTCSVREKAQEKVFH
CCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
DLGRVKHLKQLKPDLIIGVGGCVASQEGDAIVARAPYVDVVFGPQTLHRLPALIAARKQS
HHHHHHHHHHCCCCEEEECCCCEECCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHHC
GRSQVDISFPEIEKFDAMPPARVEGASAFVSIMEGCSKYCTFCVVPYTRGEEISRPLEDV
CCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHH
LAEIAGLAEQGVKEVTLLGQNVNAWRGEIVRDAGEEGDFAFLLECVAEIPGIERIRYTTS
HHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCC
HPREMTQRLFDAYVKIPKLVSQLHLPVQSGSDRILAAMKRGYSVLEFKSIVRKLRAARPD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
LSLSSDFIVGFPGETEEDFEKTMKLIDEVGFDGSFSFVYSARPGTPAADLEDPVPQETKL
CCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHH
AWLARLQKRIDEQAQAVSQSMVGSAQRILVEGVSRKSAEELMGRTDNNRVVNFPAPSPHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHH
DRLVGQFIEVRITSALPHSLRGEILTRES
HHHHHHHHHHHHHHHCCHHHCCCEECCCC
>Mature Secondary Structure
MKKLYIRTFGCQMNEYDSEKMADVLGAGEGIAKTDNPEEADVILFNTCSVREKAQEKVFH
CCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
DLGRVKHLKQLKPDLIIGVGGCVASQEGDAIVARAPYVDVVFGPQTLHRLPALIAARKQS
HHHHHHHHHHCCCCEEEECCCCEECCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHHC
GRSQVDISFPEIEKFDAMPPARVEGASAFVSIMEGCSKYCTFCVVPYTRGEEISRPLEDV
CCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHH
LAEIAGLAEQGVKEVTLLGQNVNAWRGEIVRDAGEEGDFAFLLECVAEIPGIERIRYTTS
HHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCC
HPREMTQRLFDAYVKIPKLVSQLHLPVQSGSDRILAAMKRGYSVLEFKSIVRKLRAARPD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
LSLSSDFIVGFPGETEEDFEKTMKLIDEVGFDGSFSFVYSARPGTPAADLEDPVPQETKL
CCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHH
AWLARLQKRIDEQAQAVSQSMVGSAQRILVEGVSRKSAEELMGRTDNNRVVNFPAPSPHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHH
DRLVGQFIEVRITSALPHSLRGEILTRES
HHHHHHHHHHHHHHHCCHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA