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The map label for this gene is rsmD [H]

Identifier: 119897054

GI number: 119897054

Start: 814728

End: 815249

Strand: Reverse

Name: rsmD [H]

Synonym: azo0763

Alternate gene names: 119897054

Gene position: 815249-814728 (Counterclockwise)

Preceding gene: 119897055

Following gene: 119897053

Centisome position: 18.63

GC content: 67.05

Gene sequence:

>522_bases
GTGGGCGGGACCTGGCGTTCCCGCCTGCTCGATGTCGCCGCGGTTGAGGGCCTGCGCCCCACCCCCGACCGCGTACGCGA
AACGCTGTTCAACTGGCTGGGGCAACGCCTGGACGGGCTGCGCTGCCTGGACCTCTTCGCCGGCACCGGCATTCTCGGCT
TCGAAGCCGCGTCGCGCGGGGCCGAGGCGGTCACCCTGGTGGAGCAGAACCCGCGTGCGCTCGACGCGCTGCACAAGGCC
GCAATAACCCTTCAGGCCTCGCAGGTAGAGATCGTGCGCGGCGATGCGGTAAAATTCCTGCAGAACACGACGGCCAGATT
CGACGTGGTATTTCTCGACCCACCCTACCATCAGGGCTGGCTCGACCGCCTGGAACCCTGGCTCGACCGCGTGCTCGACA
AGGACGGCTGGCTCTACGCAGAAGCTGAAACGCCGCTCGACCGGCTGGGCAAGTGGCAGACGCTCAAGCAGGGGCACGCC
GGCCTGGTCCATTACCACCTGATGCAGAGGGCAGACGAATGA

Upstream 100 bases:

>100_bases
TCGCGCGGCGGGTGCGTCCCGAGCACATGGTGACCGTGGTGGCCGGCGGCGATGGCGATACCCAGGCCAAGGCCGCGGCG
CGGTGAGCCGGGTCCGTATC

Downstream 100 bases:

>100_bases
GGGATGGGGTCGCGATCTACCCGGGGACCTTCGATCCCTTCACCCGCGGGCACGAGGACCTCGTACGACGGGCCTCGCTG
CTGTTCGATCAGGTCGTGGT

Product: putative methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MGGTWRSRLLDVAAVEGLRPTPDRVRETLFNWLGQRLDGLRCLDLFAGTGILGFEAASRGAEAVTLVEQNPRALDALHKA
AITLQASQVEIVRGDAVKFLQNTTARFDVVFLDPPYHQGWLDRLEPWLDRVLDKDGWLYAEAETPLDRLGKWQTLKQGHA
GLVHYHLMQRADE

Sequences:

>Translated_173_residues
MGGTWRSRLLDVAAVEGLRPTPDRVRETLFNWLGQRLDGLRCLDLFAGTGILGFEAASRGAEAVTLVEQNPRALDALHKA
AITLQASQVEIVRGDAVKFLQNTTARFDVVFLDPPYHQGWLDRLEPWLDRVLDKDGWLYAEAETPLDRLGKWQTLKQGHA
GLVHYHLMQRADE
>Mature_172_residues
GGTWRSRLLDVAAVEGLRPTPDRVRETLFNWLGQRLDGLRCLDLFAGTGILGFEAASRGAEAVTLVEQNPRALDALHKAA
ITLQASQVEIVRGDAVKFLQNTTARFDVVFLDPPYHQGWLDRLEPWLDRVLDKDGWLYAEAETPLDRLGKWQTLKQGHAG
LVHYHLMQRADE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=179, Percent_Identity=40.2234636871508, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 19499; Mature: 19368

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGTWRSRLLDVAAVEGLRPTPDRVRETLFNWLGQRLDGLRCLDLFAGTGILGFEAASRG
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEHHHHCCC
AEAVTLVEQNPRALDALHKAAITLQASQVEIVRGDAVKFLQNTTARFDVVFLDPPYHQGW
CCEEEEECCCCHHHHHHHHHHHEEECCCEEEEHHHHHHHHHCCCCEEEEEEECCCCCCHH
LDRLEPWLDRVLDKDGWLYAEAETPLDRLGKWQTLKQGHAGLVHYHLMQRADE
HHHHHHHHHHHHCCCCCEEEECCCCHHHHCCHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
GGTWRSRLLDVAAVEGLRPTPDRVRETLFNWLGQRLDGLRCLDLFAGTGILGFEAASRG
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEHHHHCCC
AEAVTLVEQNPRALDALHKAAITLQASQVEIVRGDAVKFLQNTTARFDVVFLDPPYHQGW
CCEEEEECCCCHHHHHHHHHHHEEECCCEEEEHHHHHHHHHCCCCEEEEEEECCCCCCHH
LDRLEPWLDRVLDKDGWLYAEAETPLDRLGKWQTLKQGHAGLVHYHLMQRADE
HHHHHHHHHHHHCCCCCEEEECCCCHHHHCCHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]