Click here to switch to the map view.

The map label for this gene is mltA [H]

Identifier: 119897016

GI number: 119897016

Start: 777974

End: 779038

Strand: Reverse

Name: mltA [H]

Synonym: azo0725

Alternate gene names: 119897016

Gene position: 779038-777974 (Counterclockwise)

Preceding gene: 119897020

Following gene: 119897008

Centisome position: 17.8

GC content: 65.35

Gene sequence:

>1065_bases
GTGGCTGAAGTTCCGCCCCACCGCGCAGCCAGCTGGACGGAAATCGACGGCTGGCGGGACGACGACCCGAGCGAAGCGTG
GTCCGCCTTTCGCAAGTCCTGCAACGTGCTAGAAAAGCAGGCGCCGTGGCAGGCCAGCTGCAAAGCCGCCCGCCAGTTGG
GAGAGGCGCCAGGCCCCGCGCAGGCACGCGCATTCTTCGAACGCCACTTCTCGCCCTGGCAGCTGGTCAATCCCGACGGC
AGCGAACAGGGCCTGATCACCGGCTATTACGAACCGCTGATCAAAGCCAGCCGTACCCCGGACGCGCTCTACCGGTGGCC
CGTGCATGGCACGCCGGGCGACCTCCTTACCATCGACCTTGCCTCCGTCTATCCGGAACTGAAGGGCTACCGGTTGCGCG
GCCGTCTGGTTGGCAACAAGGTGCTCCCGTACTGGAAGCGCGACGAGCTGGGTGACCAGGGAAGCCGCCTGCCCGCCCCG
GTGCTGCTGTGGGCCGCGGACCCCATCGATCTGTTTTTCATGCAGGTGCAGGGGTCCGGTCGCGCTGAGCTGCCAGATGG
CAGCGTGCTCCGGCTGGGATACGCGGACCAGAATGGCCACCCCTATCAATCCATTGGTCGCTGGCTCGTTCAGCAGGGGC
AGCTGAGCCTGGACAAGGCATCGATGGACGGCATCCGCGACTGGGCACGGCGACATCCGCAACGCCTGGAAGAAATGCTG
AACAGCAATCCAAGCTACGTATTCTTCCGTGAGTTGCCCTCGTCGGCTGAAGGCCCCATCGGCGCCCAAGGTGCGCCGCT
GACGGCAAACCGCAGCATCGCGATCGATTCGCGCTTCGTTGCGCTCGGCGCCCCGGTCTTTCTTGCCACCACCCAGCCGA
ATAGCGACACCCCGCTCAAGCGGCTGATGATCGCGCAGGATACCGGCGGAGCCATCAAGGGGGCAGTGAGGGCCGACTAC
TTCTGGGGTTTTGGCCCGCAGGCCGGCGCAATGGCAGGTCGAATGCGCCAGCAGGGCAGGATGTGGGTCCTGCTTCCGAA
TGGAATGAAGCCGGCGGCGAACTAA

Upstream 100 bases:

>100_bases
TTCTTCGCCGACGCTGCCGGTCGCCCCGGTCTCCACGCCACAAGCCTGTCCATCGCCTACGGTGTGCCAGGCCTGCGCCG
CATGTCCGGCGCCGCAGCCG

Downstream 100 bases:

>100_bases
CGCTCGCCGCCGGTGTGGGTGCTCAACGAGGCGTCACGCGGGCGAGCGCCTTTTCGAGTTCGGGCAACTGGACGAAGCCG
CCAATCCGCGTGCCGTCGGA

Product: putative membrane-bound transglycolase

Products: Muramic Acid Residue [C]

Alternate protein names: Mlt38; Murein hydrolase A [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MAEVPPHRAASWTEIDGWRDDDPSEAWSAFRKSCNVLEKQAPWQASCKAARQLGEAPGPAQARAFFERHFSPWQLVNPDG
SEQGLITGYYEPLIKASRTPDALYRWPVHGTPGDLLTIDLASVYPELKGYRLRGRLVGNKVLPYWKRDELGDQGSRLPAP
VLLWAADPIDLFFMQVQGSGRAELPDGSVLRLGYADQNGHPYQSIGRWLVQQGQLSLDKASMDGIRDWARRHPQRLEEML
NSNPSYVFFRELPSSAEGPIGAQGAPLTANRSIAIDSRFVALGAPVFLATTQPNSDTPLKRLMIAQDTGGAIKGAVRADY
FWGFGPQAGAMAGRMRQQGRMWVLLPNGMKPAAN

Sequences:

>Translated_354_residues
MAEVPPHRAASWTEIDGWRDDDPSEAWSAFRKSCNVLEKQAPWQASCKAARQLGEAPGPAQARAFFERHFSPWQLVNPDG
SEQGLITGYYEPLIKASRTPDALYRWPVHGTPGDLLTIDLASVYPELKGYRLRGRLVGNKVLPYWKRDELGDQGSRLPAP
VLLWAADPIDLFFMQVQGSGRAELPDGSVLRLGYADQNGHPYQSIGRWLVQQGQLSLDKASMDGIRDWARRHPQRLEEML
NSNPSYVFFRELPSSAEGPIGAQGAPLTANRSIAIDSRFVALGAPVFLATTQPNSDTPLKRLMIAQDTGGAIKGAVRADY
FWGFGPQAGAMAGRMRQQGRMWVLLPNGMKPAAN
>Mature_353_residues
AEVPPHRAASWTEIDGWRDDDPSEAWSAFRKSCNVLEKQAPWQASCKAARQLGEAPGPAQARAFFERHFSPWQLVNPDGS
EQGLITGYYEPLIKASRTPDALYRWPVHGTPGDLLTIDLASVYPELKGYRLRGRLVGNKVLPYWKRDELGDQGSRLPAPV
LLWAADPIDLFFMQVQGSGRAELPDGSVLRLGYADQNGHPYQSIGRWLVQQGQLSLDKASMDGIRDWARRHPQRLEEMLN
SNPSYVFFRELPSSAEGPIGAQGAPLTANRSIAIDSRFVALGAPVFLATTQPNSDTPLKRLMIAQDTGGAIKGAVRADYF
WGFGPQAGAMAGRMRQQGRMWVLLPNGMKPAAN

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi [H]

COG id: COG2821

COG function: function code M; Membrane-bound lytic murein transglycosylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789179, Length=279, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010611
- InterPro:   IPR014733
- InterPro:   IPR005300 [H]

Pfam domain/function: PF06725 3D; PF03562 MltA [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 39107; Mature: 38976

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVPPHRAASWTEIDGWRDDDPSEAWSAFRKSCNVLEKQAPWQASCKAARQLGEAPGPA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHH
QARAFFERHFSPWQLVNPDGSEQGLITGYYEPLIKASRTPDALYRWPVHGTPGDLLTIDL
HHHHHHHHCCCCCEEECCCCCCCCEEEHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEH
ASVYPELKGYRLRGRLVGNKVLPYWKRDELGDQGSRLPAPVLLWAADPIDLFFMQVQGSG
HHHCHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCC
RAELPDGSVLRLGYADQNGHPYQSIGRWLVQQGQLSLDKASMDGIRDWARRHPQRLEEML
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
NSNPSYVFFRELPSSAEGPIGAQGAPLTANRSIAIDSRFVALGAPVFLATTQPNSDTPLK
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCEEEEECCCCCCCCHH
RLMIAQDTGGAIKGAVRADYFWGFGPQAGAMAGRMRQQGRMWVLLPNGMKPAAN
HEEEECCCCCCEECCEEECEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
AEVPPHRAASWTEIDGWRDDDPSEAWSAFRKSCNVLEKQAPWQASCKAARQLGEAPGPA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHH
QARAFFERHFSPWQLVNPDGSEQGLITGYYEPLIKASRTPDALYRWPVHGTPGDLLTIDL
HHHHHHHHCCCCCEEECCCCCCCCEEEHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEH
ASVYPELKGYRLRGRLVGNKVLPYWKRDELGDQGSRLPAPVLLWAADPIDLFFMQVQGSG
HHHCHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCC
RAELPDGSVLRLGYADQNGHPYQSIGRWLVQQGQLSLDKASMDGIRDWARRHPQRLEEML
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
NSNPSYVFFRELPSSAEGPIGAQGAPLTANRSIAIDSRFVALGAPVFLATTQPNSDTPLK
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCEEEEECCCCCCCCHH
RLMIAQDTGGAIKGAVRADYFWGFGPQAGAMAGRMRQQGRMWVLLPNGMKPAAN
HEEEECCCCCCEECCEEECEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]