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The map label for this gene is pleD [H]

Identifier: 119896947

GI number: 119896947

Start: 689058

End: 690014

Strand: Direct

Name: pleD [H]

Synonym: azo0656

Alternate gene names: 119896947

Gene position: 689058-690014 (Clockwise)

Preceding gene: 119896946

Following gene: 119896948

Centisome position: 15.75

GC content: 71.89

Gene sequence:

>957_bases
ATGAGCCCGTCCCCCGCCACCGTCGCCGTGCCGCAGTCGGCCGGCATCGTGCTGATCGTCGATGATGTGCCGGAGAACCT
CTCGCTGCTGCACGACGCGCTCGACGAAGCCGGCTACACCGTGCTGGTCGCCACCAACGGCGAAAGCGCGCTCGCCCGCG
CCCGCCAGAGCCTGCCCGACGTGGTGCTGCTCGACGCGGTGATGCCCGGCATGGATGGCTTCGAGGTGGCGCGGCGGCTC
AAGGCCGACTTCGTCACCCGCGCCATCCCGCTGATCTTCATGACCGGCCTCACCGAGACCGAGCACGTGGTCGCCGCGTT
CGCCGCCGGCGGCGCCGACTACGTCACCAAGCCGATCAAGCCGGCCGAGGTGCTGGCGCGCATCGCCGCCCACGTGCAGA
ACGCGCGCCAGATGAAGCAGGCGCGCAGCGCGCTCGACGCCTTCGGCCAGGCCACCGTGGCGGTGCGCGCCACCGACGGC
AAGCTGGTCTGGCAGACGCCGCTCGCGCGCCAGCTGATCCGCAACTGGTTCGACATCGGCGCCGACGAAACCACGCCGCC
GCGGCTGATCGAGTGGATCCTCGCCGCGGAACTCGCGCGCCGCGAACGCCGCGAGGTCGCGCCGCTGGTGATGGCCGACG
GCGCCCGCCGGCTGCTCGCCTCGTTCCACGACCACACCGGCGAGGAGGAGTGGCTGGTGGTGCTGCGCGAGGAGAACGAC
GCCAGCGCGATCGAGGCCCTGATCGCCGCCTTCCGCCTCACCACGCGCGAGGCCGAGGTGCTGTACTGGGTCACCCGAGG
CAAGACCAACCGCGACATCGGCGACATCCTCGGCTCCAGCCCGCGCACGGTGCACAAGCACCTGGAGCATGTGTTCGAGA
AGCTCGGCGTGGAAACCCGCACCGCGGCCGCGTCGATGGCGCTGGCGAAGATCCGCGGCGCGGGCGAGGGCGGCTGA

Upstream 100 bases:

>100_bases
CACCCGATTACGCCGAGTTCGTGCGCGTGCAGCGCGAACTCGCGCGCCAGTTCCAGTTCGACGCCATGCACGAAATCCTG
CGCCGGAGCGAAGCCCCGCA

Downstream 100 bases:

>100_bases
GGGCCGGGCCGCTTTGCGCCCGGCTCTGGTGAGGCAGTCGTTTCGGCTTGCATCGTTTTCCGCCTCCTGAACGTTGGAGT
CTGGCAGGGACGCTCGGGCT

Product: LuxR family transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSPSPATVAVPQSAGIVLIVDDVPENLSLLHDALDEAGYTVLVATNGESALARARQSLPDVVLLDAVMPGMDGFEVARRL
KADFVTRAIPLIFMTGLTETEHVVAAFAAGGADYVTKPIKPAEVLARIAAHVQNARQMKQARSALDAFGQATVAVRATDG
KLVWQTPLARQLIRNWFDIGADETTPPRLIEWILAAELARRERREVAPLVMADGARRLLASFHDHTGEEEWLVVLREEND
ASAIEALIAAFRLTTREAEVLYWVTRGKTNRDIGDILGSSPRTVHKHLEHVFEKLGVETRTAAASMALAKIRGAGEGG

Sequences:

>Translated_318_residues
MSPSPATVAVPQSAGIVLIVDDVPENLSLLHDALDEAGYTVLVATNGESALARARQSLPDVVLLDAVMPGMDGFEVARRL
KADFVTRAIPLIFMTGLTETEHVVAAFAAGGADYVTKPIKPAEVLARIAAHVQNARQMKQARSALDAFGQATVAVRATDG
KLVWQTPLARQLIRNWFDIGADETTPPRLIEWILAAELARRERREVAPLVMADGARRLLASFHDHTGEEEWLVVLREEND
ASAIEALIAAFRLTTREAEVLYWVTRGKTNRDIGDILGSSPRTVHKHLEHVFEKLGVETRTAAASMALAKIRGAGEGG
>Mature_317_residues
SPSPATVAVPQSAGIVLIVDDVPENLSLLHDALDEAGYTVLVATNGESALARARQSLPDVVLLDAVMPGMDGFEVARRLK
ADFVTRAIPLIFMTGLTETEHVVAAFAAGGADYVTKPIKPAEVLARIAAHVQNARQMKQARSALDAFGQATVAVRATDGK
LVWQTPLARQLIRNWFDIGADETTPPRLIEWILAAELARRERREVAPLVMADGARRLLASFHDHTGEEEWLVVLREENDA
SAIEALIAAFRLTTREAEVLYWVTRGKTNRDIGDILGSSPRTVHKHLEHVFEKLGVETRTAAASMALAKIRGAGEGG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788394, Length=124, Percent_Identity=31.4516129032258, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790860, Length=105, Percent_Identity=34.2857142857143, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786784, Length=112, Percent_Identity=33.0357142857143, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=32.5581395348837, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=34.8148148148148, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790437, Length=169, Percent_Identity=28.4023668639053, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87082012, Length=133, Percent_Identity=27.8195488721804, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789809, Length=143, Percent_Identity=31.4685314685315, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34460; Mature: 34329

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSPATVAVPQSAGIVLIVDDVPENLSLLHDALDEAGYTVLVATNGESALARARQSLPD
CCCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCC
VVLLDAVMPGMDGFEVARRLKADFVTRAIPLIFMTGLTETEHVVAAFAAGGADYVTKPIK
EEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCC
PAEVLARIAAHVQNARQMKQARSALDAFGQATVAVRATDGKLVWQTPLARQLIRNWFDIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEECCHHHHHHHHHHHHCC
ADETTPPRLIEWILAAELARRERREVAPLVMADGARRLLASFHDHTGEEEWLVVLREEND
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
ASAIEALIAAFRLTTREAEVLYWVTRGKTNRDIGDILGSSPRTVHKHLEHVFEKLGVETR
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHH
TAAASMALAKIRGAGEGG
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SPSPATVAVPQSAGIVLIVDDVPENLSLLHDALDEAGYTVLVATNGESALARARQSLPD
CCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCC
VVLLDAVMPGMDGFEVARRLKADFVTRAIPLIFMTGLTETEHVVAAFAAGGADYVTKPIK
EEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCC
PAEVLARIAAHVQNARQMKQARSALDAFGQATVAVRATDGKLVWQTPLARQLIRNWFDIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEECCHHHHHHHHHHHHCC
ADETTPPRLIEWILAAELARRERREVAPLVMADGARRLLASFHDHTGEEEWLVVLREEND
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
ASAIEALIAAFRLTTREAEVLYWVTRGKTNRDIGDILGSSPRTVHKHLEHVFEKLGVETR
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHH
TAAASMALAKIRGAGEGG
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA