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The map label for this gene is oprM2 [H]

Identifier: 119896901

GI number: 119896901

Start: 638132

End: 639553

Strand: Reverse

Name: oprM2 [H]

Synonym: azo0610

Alternate gene names: 119896901

Gene position: 639553-638132 (Counterclockwise)

Preceding gene: 119896902

Following gene: 119896897

Centisome position: 14.61

GC content: 73.42

Gene sequence:

>1422_bases
ATGACCGCTTCCGTCGCGCGCCTGCGCCTTGCCCCCGTGCTGCTCGCCGCGCTGGTCGCCGGCTGCGCCTTCACCGAACC
GGTCGCGCGCCCCGACCAGCCGCTACCCGCGCAGTGGACCGAACAGCCCGGCCCTGCCGCCGCCACCCCACTGCCCGACA
CCTGGTGGCAGTCCTTCGGCTCGCCGGCACTGGACGCGCTGGTGGCCGAGGCGCTGGTCGCCAGCCCAGACCTGCAGGTG
CAGGCGGAGCGCGTGCTCCAGGCCGAACTCGCGCTGCGCCAGACGCGTGCGTCGCTGTTCCCGTGGCTCACGCTCGACGC
CGACAGCGGCTGGCGCCGCGCCGACGCCGGCGACCGCGGCGCGAGCACGGTGACCGAAACCAAGACCACCTCGCTCGGCC
TTTCCGCCAGTTACGAGGTCGATCTGTGGGGCCGCGTGGCCGCCAACGTGGGCAGCGCGCGCGCCAGCCTCAACGCCACC
CGCTACGACCGCGACAGCGTGCGCCTGTCGCTCGCCGCGAGCGTCGCCACCACCTATTTCCAGCTGCTGACGCTGCAGGA
GCGGCTGGAGATCGCACGCCAGAACCTGGCGATTGCCGAACGCGTGCTGCGCGTGGTCGAGGCGCGCTACCGCAACGGGG
CCGCGTCGGCACTGGAAGTGAGCCAGCAGCGCACCACGGTGCTCACCCAGCGCGCTGCGATCGAACCGCTGGAGGTCAGC
GTGCGCCAGACGCGCTCCGCGCTCGCCATCCTGCTCGGCCGCAATCCGCAGGACGCCGCGCCGGAATTCGAACGCCTCGA
AGCGCTGGCCATCCCCACGGTGACACCCGGCCTGCCCGTCGAGCTGCTGCTGCGCCGGCCCGATCTGGCGAGCGTGGAAG
CCAGTCTCGCCGCCGCCTCCGCCGACATCGCCGCGGCGCGTGCCGCCTTGCTGCCCAGCATCAGCCTGTCGGCCGGCGGC
GGCGTGGCCAGCAGCCTGCTGCTGTCGCTGGCCGACCCGGGCACCACGGTGTCGCTGTCGGCCTCGCTGGTGCAGACCAT
CTTCGACGGCGGCCGCCTGCAGGCCGCGGTCGACATCGCCCGCTCGCGCCAGCGCGAACTGCTCGAGAGCTACCGCAGCG
CGATCATCACCGCGTTGAAGGAAGTGGAAGACGCGCTCGGCAACGCAAGCCGGGACGCGAATCAGGAAGCCGCGCAGCGC
GAGATCCTCGCCGAAGCCCAGCGCGCGCTGCGTCTGGCCGAGCTGCGCTACCGCGAGGGCGCGGCCGACCTGCTCACCGT
CCTGGACGCCCAGCGCACGCTGTTCTCGGCGCAGGACCAGCTGGCCCAGCTGCGCCAGGCGCGGTTGACCGATGCGGTCG
GGCTGTACAAGGCGCTCGGGGGCGGCTGGCGGGCGGAGAACTCGATGGCGGCGGGCGGCTGA

Upstream 100 bases:

>100_bases
CCTTCGCCACCGGCCTGGTATTCGGCTACCTGCCCGCACGCAAGGCCGCACGGCTGGACCCGGTGGTCGCCCTCGCTTCC
GAATGACAGTTCCACACACC

Downstream 100 bases:

>100_bases
GGCAGCCCGCTCAGCGCGGGGCCGCCAGCAGCGCCTGCGGCCCGATGTCCGCCAGCGTCGCGCAGCCGGCGAGCGCCATC
GCCACTTCCAGCTCGTCGCG

Product: putative outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTASVARLRLAPVLLAALVAGCAFTEPVARPDQPLPAQWTEQPGPAAATPLPDTWWQSFGSPALDALVAEALVASPDLQV
QAERVLQAELALRQTRASLFPWLTLDADSGWRRADAGDRGASTVTETKTTSLGLSASYEVDLWGRVAANVGSARASLNAT
RYDRDSVRLSLAASVATTYFQLLTLQERLEIARQNLAIAERVLRVVEARYRNGAASALEVSQQRTTVLTQRAAIEPLEVS
VRQTRSALAILLGRNPQDAAPEFERLEALAIPTVTPGLPVELLLRRPDLASVEASLAAASADIAAARAALLPSISLSAGG
GVASSLLLSLADPGTTVSLSASLVQTIFDGGRLQAAVDIARSRQRELLESYRSAIITALKEVEDALGNASRDANQEAAQR
EILAEAQRALRLAELRYREGAADLLTVLDAQRTLFSAQDQLAQLRQARLTDAVGLYKALGGGWRAENSMAAGG

Sequences:

>Translated_473_residues
MTASVARLRLAPVLLAALVAGCAFTEPVARPDQPLPAQWTEQPGPAAATPLPDTWWQSFGSPALDALVAEALVASPDLQV
QAERVLQAELALRQTRASLFPWLTLDADSGWRRADAGDRGASTVTETKTTSLGLSASYEVDLWGRVAANVGSARASLNAT
RYDRDSVRLSLAASVATTYFQLLTLQERLEIARQNLAIAERVLRVVEARYRNGAASALEVSQQRTTVLTQRAAIEPLEVS
VRQTRSALAILLGRNPQDAAPEFERLEALAIPTVTPGLPVELLLRRPDLASVEASLAAASADIAAARAALLPSISLSAGG
GVASSLLLSLADPGTTVSLSASLVQTIFDGGRLQAAVDIARSRQRELLESYRSAIITALKEVEDALGNASRDANQEAAQR
EILAEAQRALRLAELRYREGAADLLTVLDAQRTLFSAQDQLAQLRQARLTDAVGLYKALGGGWRAENSMAAGG
>Mature_472_residues
TASVARLRLAPVLLAALVAGCAFTEPVARPDQPLPAQWTEQPGPAAATPLPDTWWQSFGSPALDALVAEALVASPDLQVQ
AERVLQAELALRQTRASLFPWLTLDADSGWRRADAGDRGASTVTETKTTSLGLSASYEVDLWGRVAANVGSARASLNATR
YDRDSVRLSLAASVATTYFQLLTLQERLEIARQNLAIAERVLRVVEARYRNGAASALEVSQQRTTVLTQRAAIEPLEVSV
RQTRSALAILLGRNPQDAAPEFERLEALAIPTVTPGLPVELLLRRPDLASVEASLAAASADIAAARAALLPSISLSAGGG
VASSLLLSLADPGTTVSLSASLVQTIFDGGRLQAAVDIARSRQRELLESYRSAIITALKEVEDALGNASRDANQEAAQRE
ILAEAQRALRLAELRYREGAADLLTVLDAQRTLFSAQDQLAQLRQARLTDAVGLYKALGGGWRAENSMAAGG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=461, Percent_Identity=33.4056399132321, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI2367348, Length=495, Percent_Identity=27.4747474747475, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50235; Mature: 50104

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASVARLRLAPVLLAALVAGCAFTEPVARPDQPLPAQWTEQPGPAAATPLPDTWWQSFG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC
SPALDALVAEALVASPDLQVQAERVLQAELALRQTRASLFPWLTLDADSGWRRADAGDRG
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCC
ASTVTETKTTSLGLSASYEVDLWGRVAANVGSARASLNATRYDRDSVRLSLAASVATTYF
CCCHHHCCCCEECCCCCEEEEHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QLLTLQERLEIARQNLAIAERVLRVVEARYRNGAASALEVSQQRTTVLTQRAAIEPLEVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
VRQTRSALAILLGRNPQDAAPEFERLEALAIPTVTPGLPVELLLRRPDLASVEASLAAAS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
ADIAAARAALLPSISLSAGGGVASSLLLSLADPGTTVSLSASLVQTIFDGGRLQAAVDIA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCHHHHHHHH
RSRQRELLESYRSAIITALKEVEDALGNASRDANQEAAQREILAEAQRALRLAELRYREG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
AADLLTVLDAQRTLFSAQDQLAQLRQARLTDAVGLYKALGGGWRAENSMAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TASVARLRLAPVLLAALVAGCAFTEPVARPDQPLPAQWTEQPGPAAATPLPDTWWQSFG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC
SPALDALVAEALVASPDLQVQAERVLQAELALRQTRASLFPWLTLDADSGWRRADAGDRG
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCC
ASTVTETKTTSLGLSASYEVDLWGRVAANVGSARASLNATRYDRDSVRLSLAASVATTYF
CCCHHHCCCCEECCCCCEEEEHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QLLTLQERLEIARQNLAIAERVLRVVEARYRNGAASALEVSQQRTTVLTQRAAIEPLEVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
VRQTRSALAILLGRNPQDAAPEFERLEALAIPTVTPGLPVELLLRRPDLASVEASLAAAS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
ADIAAARAALLPSISLSAGGGVASSLLLSLADPGTTVSLSASLVQTIFDGGRLQAAVDIA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCHHHHHHHH
RSRQRELLESYRSAIITALKEVEDALGNASRDANQEAAQREILAEAQRALRLAELRYREG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
AADLLTVLDAQRTLFSAQDQLAQLRQARLTDAVGLYKALGGGWRAENSMAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]