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The map label for this gene is rlmI [H]

Identifier: 119896889

GI number: 119896889

Start: 626914

End: 628101

Strand: Direct

Name: rlmI [H]

Synonym: azo0598

Alternate gene names: 119896889

Gene position: 626914-628101 (Clockwise)

Preceding gene: 119896888

Following gene: 119896894

Centisome position: 14.33

GC content: 69.7

Gene sequence:

>1188_bases
ATGGCCCGTCTGTTTCTGCATCCCGGCAAGGAGCGCTCGCTGCTCCGCCGCCATCCCTGGATTTTTGCCGGTTCCGTCGA
ACGCCTCGAAGGGCGTGCTCGCGCGGGGGACACGGTTGACGTGCTGTCGGCCGACGGCCGCGTGCTGGGCCGGGCCGCAT
GGTCGCCGGTGTCGCAGATCCGCGCCCGCATGTGGTGTTTCGACGCCGATACCACGATCGACCACGCCTTCTTCAAGCGC
CGCGTGGCGGCGGCCGTGGCGGCCCGGCAGAGTCACCCGCTGCTGACGGGGCAGGACGGCCTGCGCCTGATTCACGGCGA
GTCCGACGGCCTGCCCGGTGTGATCGCCGACCGTTACGGCGACGTCGTGGTGGTGCAACTCACCAGCGCCGGGGCGGACA
AGTGGCGCGACGCGATCGTCGCTGCGCTGGTCCAGGCCACGGGCTGCAGTACCGTCTATGAACGCTCGGATTCCGACGTC
CGCGGCCTCGAAGGGCTGGAGCCGAAAACCGGCTGCGTGCACGGCGCGCTGCCGGAGACCTTGCAGATCGTCGAGCACGG
GGTGCGCATGGAGGTCGATGTCGCCGGTGGCCACAAGACCGGCTTCTATCTCGACCAGCGCGACAACCGCCTGCTCACCG
GGCGCCTCGCGCGCGGGCGGCGGGTGCTGAACTGCTTCTGCTACACCGGCGGCTTCTCGCTGCAGGCATTGGCCGGGGGC
GCGGAAAGCGTGTTGTCCATCGACTCTTCCGGCCCCGCGCTCGCAACCGCGGCGCGCAACCTCGCGCTCAACCCGCAACT
GGATGCCGCGCGGGCGGACTGGTGGGAAGAGGATGTGTTCGAGGCGCTGCGGGCACTGCGCGCCGAAGGCCGCAGCTTCG
ACCTGATCGTGCTCGATCCGCCCAAGTTCGCGCCTTCGGCCGCGCACGCCGAGCGCGCAGCGCGCGCCTACAAGGACATC
AACCTGTTCGGCTTCCGCCTGCTGGCGCCGGGCGGCATCCTGATGACCTATTCCTGCTCGGGCGGCATCGGTCTGGAGCT
GTTCCAGAAGATCGTTGCCGGCGCGGCGTCGGACGCCGGTGTCGATGCGCGCATCCTGCATCGCCTGTCGGCGGCGCCGG
ACCACCCGATCGGGCTGTCGGTGCCGGAGGGTGAATACCTGAAAGGGCTGGCGATCCAGATCGCCTAA

Upstream 100 bases:

>100_bases
CCGCGGACCACGCCCGCCGCCTGCTCGAGTCCTGACGCCGGCAATTGGCCGTGGAGCGGGCGGCCGCTAAACTGTCCGCC
CGCTTTCCGTCGTGATTCCC

Downstream 100 bases:

>100_bases
CGGGCACTGGCCGGCGGCCGGTAGTCCGGGTTGGGGCCGGGCGGCGCGAGGTTGAAGGCGGCGCTGAGCAGCACCAGCGC
CAGCACCAGCACCGCGAAAT

Product: SAM dependent methyl transferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MARLFLHPGKERSLLRRHPWIFAGSVERLEGRARAGDTVDVLSADGRVLGRAAWSPVSQIRARMWCFDADTTIDHAFFKR
RVAAAVAARQSHPLLTGQDGLRLIHGESDGLPGVIADRYGDVVVVQLTSAGADKWRDAIVAALVQATGCSTVYERSDSDV
RGLEGLEPKTGCVHGALPETLQIVEHGVRMEVDVAGGHKTGFYLDQRDNRLLTGRLARGRRVLNCFCYTGGFSLQALAGG
AESVLSIDSSGPALATAARNLALNPQLDAARADWWEEDVFEALRALRAEGRSFDLIVLDPPKFAPSAAHAERAARAYKDI
NLFGFRLLAPGGILMTYSCSGGIGLELFQKIVAGAASDAGVDARILHRLSAAPDHPIGLSVPEGEYLKGLAIQIA

Sequences:

>Translated_395_residues
MARLFLHPGKERSLLRRHPWIFAGSVERLEGRARAGDTVDVLSADGRVLGRAAWSPVSQIRARMWCFDADTTIDHAFFKR
RVAAAVAARQSHPLLTGQDGLRLIHGESDGLPGVIADRYGDVVVVQLTSAGADKWRDAIVAALVQATGCSTVYERSDSDV
RGLEGLEPKTGCVHGALPETLQIVEHGVRMEVDVAGGHKTGFYLDQRDNRLLTGRLARGRRVLNCFCYTGGFSLQALAGG
AESVLSIDSSGPALATAARNLALNPQLDAARADWWEEDVFEALRALRAEGRSFDLIVLDPPKFAPSAAHAERAARAYKDI
NLFGFRLLAPGGILMTYSCSGGIGLELFQKIVAGAASDAGVDARILHRLSAAPDHPIGLSVPEGEYLKGLAIQIA
>Mature_394_residues
ARLFLHPGKERSLLRRHPWIFAGSVERLEGRARAGDTVDVLSADGRVLGRAAWSPVSQIRARMWCFDADTTIDHAFFKRR
VAAAVAARQSHPLLTGQDGLRLIHGESDGLPGVIADRYGDVVVVQLTSAGADKWRDAIVAALVQATGCSTVYERSDSDVR
GLEGLEPKTGCVHGALPETLQIVEHGVRMEVDVAGGHKTGFYLDQRDNRLLTGRLARGRRVLNCFCYTGGFSLQALAGGA
ESVLSIDSSGPALATAARNLALNPQLDAARADWWEEDVFEALRALRAEGRSFDLIVLDPPKFAPSAAHAERAARAYKDIN
LFGFRLLAPGGILMTYSCSGGIGLELFQKIVAGAASDAGVDARILHRLSAAPDHPIGLSVPEGEYLKGLAIQIA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=393, Percent_Identity=49.8727735368957, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1787181, Length=271, Percent_Identity=30.2583025830258, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 42391; Mature: 42260

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLFLHPGKERSLLRRHPWIFAGSVERLEGRARAGDTVDVLSADGRVLGRAAWSPVSQI
CCEEEECCCCHHHHHHHCCEEEECCHHHHCCCCCCCCEEEEEECCCCEEEHHHCCCHHHH
RARMWCFDADTTIDHAFFKRRVAAAVAARQSHPLLTGQDGLRLIHGESDGLPGVIADRYG
HHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCCCEEHHCCC
DVVVVQLTSAGADKWRDAIVAALVQATGCSTVYERSDSDVRGLEGLEPKTGCVHGALPET
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
LQIVEHGVRMEVDVAGGHKTGFYLDQRDNRLLTGRLARGRRVLNCFCYTGGFSLQALAGG
HHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEHHHHCCCEEEEEEEEECCCEEEECCCC
AESVLSIDSSGPALATAARNLALNPQLDAARADWWEEDVFEALRALRAEGRSFDLIVLDP
CCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEEECC
PKFAPSAAHAERAARAYKDINLFGFRLLAPGGILMTYSCSGGIGLELFQKIVAGAASDAG
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
VDARILHRLSAAPDHPIGLSVPEGEYLKGLAIQIA
CHHHHHHHHHCCCCCCCCCCCCCCHHHCCEEEEEC
>Mature Secondary Structure 
ARLFLHPGKERSLLRRHPWIFAGSVERLEGRARAGDTVDVLSADGRVLGRAAWSPVSQI
CEEEECCCCHHHHHHHCCEEEECCHHHHCCCCCCCCEEEEEECCCCEEEHHHCCCHHHH
RARMWCFDADTTIDHAFFKRRVAAAVAARQSHPLLTGQDGLRLIHGESDGLPGVIADRYG
HHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCCCEEHHCCC
DVVVVQLTSAGADKWRDAIVAALVQATGCSTVYERSDSDVRGLEGLEPKTGCVHGALPET
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
LQIVEHGVRMEVDVAGGHKTGFYLDQRDNRLLTGRLARGRRVLNCFCYTGGFSLQALAGG
HHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEHHHHCCCEEEEEEEEECCCEEEECCCC
AESVLSIDSSGPALATAARNLALNPQLDAARADWWEEDVFEALRALRAEGRSFDLIVLDP
CCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEEECC
PKFAPSAAHAERAARAYKDINLFGFRLLAPGGILMTYSCSGGIGLELFQKIVAGAASDAG
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
VDARILHRLSAAPDHPIGLSVPEGEYLKGLAIQIA
CHHHHHHHHHCCCCCCCCCCCCCCHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA