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The map label for this gene is dapF

Identifier: 119896885

GI number: 119896885

Start: 623683

End: 624516

Strand: Direct

Name: dapF

Synonym: azo0594

Alternate gene names: 119896885

Gene position: 623683-624516 (Clockwise)

Preceding gene: 119896883

Following gene: 119896886

Centisome position: 14.25

GC content: 69.78

Gene sequence:

>834_bases
ATGCATATCCGCTTCACCAAGATGCACGGCCTCGGTAACGACTTCGTCGTCATCGACGCCACCCGTACGCCGGTCGACCT
CACTCCCGCGCGGGTCAAGGCGATCGCCGACCGCCATTTCGGCGTCGGTTGCGACCAGTTGCTGGTGGTCGAGCCGCCGG
GCAGCGACGAAGTGGATTTCCGCTACCGCATCTTCAACGCCGACGGCGGCGAGGTGGAGCAGTGCGGCAACGGCGCGCGC
TGCTTCGTCCGCTTCGTGCATGACAAGGGCCTGACCGACAAGCGCGAAATCCGCGTGGAAACCCGCGCCGGCGTCATCGC
TCCCGCGCTGCGCCCGGACGGGCTGGTGTCGGTGGACATGGGCGTGCCCGAGCTGAGTCCGGCGCGCATTCCCTTCGTGT
CCGATTCCGACGCCTGGGTGCAGCCGCTGCAACTGGCGGAAGACACGGTGGCGATCACCGCGGTGTCGATGGGCAACCCG
CACGCGGTGCAGGTGGTCGCCGACGTCGACACTGCCCCGGTGGCCCGCCAGGGCGCCGAAATCGAATGCCATCCGCGCTT
TCCGGCCCGGGTCAACGCCGGCTTCATGCAGGTGGTCGACGCCGGCCACATCCGCCTGCGCGTATTCGAACGCGGTGCGG
GCGAAACGCTGGCCTGCGGCACCGGCGCGTGCGCCGCGGTGGTGGCGGGCATCGGGCGCGGCCTGCTGGTGTCGCCGGTC
CGCGTCGAAACCCGCGGCGGCGAACTCGAAATCGCGTGGGCGGGGCCGGGTACGCCGGTCGTGATGACCGGCCCGGCGGT
CACGGTTTTCGAAGGCGAACTCGAGCTTCCCTGA

Upstream 100 bases:

>100_bases
CCGGCAAGCGCGCGTAGGCCCGCACCCGACGCGGGCGGCCGCGACGCAGTGGTTCCAGCCGACGCCGCCCCCTTTCAGCT
TCCAACCGGATGTTTCCCGC

Downstream 100 bases:

>100_bases
TCCCATCTTCGCGGCTGCGCGCAGCCGCATGGAGCACCGCATGAACCCTAACGACGTCGCCCGCTACCTGCGCGACAACC
CTGACTTCCTCGCCCAGCAC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MHIRFTKMHGLGNDFVVIDATRTPVDLTPARVKAIADRHFGVGCDQLLVVEPPGSDEVDFRYRIFNADGGEVEQCGNGAR
CFVRFVHDKGLTDKREIRVETRAGVIAPALRPDGLVSVDMGVPELSPARIPFVSDSDAWVQPLQLAEDTVAITAVSMGNP
HAVQVVADVDTAPVARQGAEIECHPRFPARVNAGFMQVVDAGHIRLRVFERGAGETLACGTGACAAVVAGIGRGLLVSPV
RVETRGGELEIAWAGPGTPVVMTGPAVTVFEGELELP

Sequences:

>Translated_277_residues
MHIRFTKMHGLGNDFVVIDATRTPVDLTPARVKAIADRHFGVGCDQLLVVEPPGSDEVDFRYRIFNADGGEVEQCGNGAR
CFVRFVHDKGLTDKREIRVETRAGVIAPALRPDGLVSVDMGVPELSPARIPFVSDSDAWVQPLQLAEDTVAITAVSMGNP
HAVQVVADVDTAPVARQGAEIECHPRFPARVNAGFMQVVDAGHIRLRVFERGAGETLACGTGACAAVVAGIGRGLLVSPV
RVETRGGELEIAWAGPGTPVVMTGPAVTVFEGELELP
>Mature_277_residues
MHIRFTKMHGLGNDFVVIDATRTPVDLTPARVKAIADRHFGVGCDQLLVVEPPGSDEVDFRYRIFNADGGEVEQCGNGAR
CFVRFVHDKGLTDKREIRVETRAGVIAPALRPDGLVSVDMGVPELSPARIPFVSDSDAWVQPLQLAEDTVAITAVSMGNP
HAVQVVADVDTAPVARQGAEIECHPRFPARVNAGFMQVVDAGHIRLRVFERGAGETLACGTGACAAVVAGIGRGLLVSPV
RVETRGGELEIAWAGPGTPVVMTGPAVTVFEGELELP

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=58.029197080292, Blast_Score=316, Evalue=9e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_AZOSB (A1K306)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932098.1
- ProteinModelPortal:   A1K306
- SMR:   A1K306
- STRING:   A1K306
- GeneID:   4606464
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0594
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   A1K306
- ProtClustDB:   PRK00450
- BioCyc:   ASP62928:AZO0594-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 29433; Mature: 29433

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIRFTKMHGLGNDFVVIDATRTPVDLTPARVKAIADRHFGVGCDQLLVVEPPGSDEVDF
CEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEE
RYRIFNADGGEVEQCGNGARCFVRFVHDKGLTDKREIRVETRAGVIAPALRPDGLVSVDM
EEEEEECCCCCHHHCCCCCEEEEEEEECCCCCCCCEEEEEECCCEEEECCCCCCEEEEEC
GVPELSPARIPFVSDSDAWVQPLQLAEDTVAITAVSMGNPHAVQVVADVDTAPVARQGAE
CCCCCCCCCCEEECCCCHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCCCCHHHCCCC
IECHPRFPARVNAGFMQVVDAGHIRLRVFERGAGETLACGTGACAAVVAGIGRGLLVSPV
EEECCCCCCEECCCEEEEECCCCEEEEEEECCCCCEEEECCCHHHHHHHHCCCCEEECCE
RVETRGGELEIAWAGPGTPVVMTGPAVTVFEGELELP
EEEECCCEEEEEECCCCCCEEEECCEEEEEECEECCC
>Mature Secondary Structure
MHIRFTKMHGLGNDFVVIDATRTPVDLTPARVKAIADRHFGVGCDQLLVVEPPGSDEVDF
CEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEE
RYRIFNADGGEVEQCGNGARCFVRFVHDKGLTDKREIRVETRAGVIAPALRPDGLVSVDM
EEEEEECCCCCHHHCCCCCEEEEEEEECCCCCCCCEEEEEECCCEEEECCCCCCEEEEEC
GVPELSPARIPFVSDSDAWVQPLQLAEDTVAITAVSMGNPHAVQVVADVDTAPVARQGAE
CCCCCCCCCCEEECCCCHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCCCCHHHCCCC
IECHPRFPARVNAGFMQVVDAGHIRLRVFERGAGETLACGTGACAAVVAGIGRGLLVSPV
EEECCCCCCEECCCEEEEECCCCEEEEEEECCCCCEEEECCCHHHHHHHHCCCCEEECCE
RVETRGGELEIAWAGPGTPVVMTGPAVTVFEGELELP
EEEECCCEEEEEECCCCCCEEEECCEEEEEECEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA