Click here to switch to the map view.

The map label for this gene is leuE [H]

Identifier: 119896745

GI number: 119896745

Start: 485487

End: 486122

Strand: Reverse

Name: leuE [H]

Synonym: azo0454

Alternate gene names: 119896745

Gene position: 486122-485487 (Counterclockwise)

Preceding gene: 119896751

Following gene: 119896744

Centisome position: 11.11

GC content: 72.48

Gene sequence:

>636_bases
ATGCCCAGCTTCGACACCCTCGCCGCCTTCCTCGCCATCTCGGTGGCGATCACCTTTGCCCCCGGGCCGGACAACCTGAT
GGTGCTCGGGCACAGCCTGGCGCGTGGGCGGCTGGCGGGGTTCGGCATTGCGCTGGGCTGCGCGCTCGGCTGCTTCACAC
ACACGCTGTGGGCGGCGCTGGGGGTGAGTGCGGCGCTGGCCTCGTCGCCGCAGGTCTTCCTTGCGCTCAAGCTCGCGGGT
GCCGCCTATCTCGCCTGGCTGGGCGTGCAGGCGCTGCGCTTTGCCGCGGCGCCACGGCTGGACGGCGTCGCCGCGACGCG
GGCGCCATGGCTGCGCGACGTGCGCCGCGGCTTCGTCGCCAATGCAATCAACCCCAAGGTGGCGCTGTTCTTCCTCGCCT
TCCTGCCGCAGTTCGTCAGCGCCGACGGCGGCCATGCGACGCTGCAGATGGTGGCGCTCGGCAGCCTGTTCGCCGTGCAG
ACGGTGCTGGTGTTCGGCGGTTTCGCGCTGGCCGCCGGCACGCTCGGCCGCGCCATCGCCCGCCGCCCGGCGGCCGGTGC
CTGGCTGGACCGCATCGCCGGGCTGATCTTCATCGGCCTCGCGCTGCGGCTCGCGACCGACGACCACACCCTATAA

Upstream 100 bases:

>100_bases
AACCCGACCGCCTGCTCCACCCCTCATGCAAGGGCCCCGCCCGGTTTGACGCCACCCCGCCCCCGCAACAGAATCCCCCC
TCCCGCCGTACACCGCCGCC

Downstream 100 bases:

>100_bases
GAAACCAGAGAACCGCTCCCCGATGAGACGCGCCCCCGCCCCGCTACCCGCCCCGGCCAGCGGCGAACGCCACGACTGGC
AGACGCTGAAGAGCCTCGTC

Product: putative threonine efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MPSFDTLAAFLAISVAITFAPGPDNLMVLGHSLARGRLAGFGIALGCALGCFTHTLWAALGVSAALASSPQVFLALKLAG
AAYLAWLGVQALRFAAAPRLDGVAATRAPWLRDVRRGFVANAINPKVALFFLAFLPQFVSADGGHATLQMVALGSLFAVQ
TVLVFGGFALAAGTLGRAIARRPAAGAWLDRIAGLIFIGLALRLATDDHTL

Sequences:

>Translated_211_residues
MPSFDTLAAFLAISVAITFAPGPDNLMVLGHSLARGRLAGFGIALGCALGCFTHTLWAALGVSAALASSPQVFLALKLAG
AAYLAWLGVQALRFAAAPRLDGVAATRAPWLRDVRRGFVANAINPKVALFFLAFLPQFVSADGGHATLQMVALGSLFAVQ
TVLVFGGFALAAGTLGRAIARRPAAGAWLDRIAGLIFIGLALRLATDDHTL
>Mature_210_residues
PSFDTLAAFLAISVAITFAPGPDNLMVLGHSLARGRLAGFGIALGCALGCFTHTLWAALGVSAALASSPQVFLALKLAGA
AYLAWLGVQALRFAAAPRLDGVAATRAPWLRDVRRGFVANAINPKVALFFLAFLPQFVSADGGHATLQMVALGSLFAVQT
VLVFGGFALAAGTLGRAIARRPAAGAWLDRIAGLIFIGLALRLATDDHTL

Specific function: Exporter of leucine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=205, Percent_Identity=30.2439024390244, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788099, Length=199, Percent_Identity=25.1256281407035, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786522, Length=193, Percent_Identity=27.979274611399, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21796; Mature: 21665

Theoretical pI: Translated: 11.56; Mature: 11.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFDTLAAFLAISVAITFAPGPDNLMVLGHSLARGRLAGFGIALGCALGCFTHTLWAAL
CCCHHHHHHHHHHHHHEEECCCCCCEEEECHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
GVSAALASSPQVFLALKLAGAAYLAWLGVQALRFAAAPRLDGVAATRAPWLRDVRRGFVA
HHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHH
NAINPKVALFFLAFLPQFVSADGGHATLQMVALGSLFAVQTVLVFGGFALAAGTLGRAIA
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRPAAGAWLDRIAGLIFIGLALRLATDDHTL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PSFDTLAAFLAISVAITFAPGPDNLMVLGHSLARGRLAGFGIALGCALGCFTHTLWAAL
CCHHHHHHHHHHHHHEEECCCCCCEEEECHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
GVSAALASSPQVFLALKLAGAAYLAWLGVQALRFAAAPRLDGVAATRAPWLRDVRRGFVA
HHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHH
NAINPKVALFFLAFLPQFVSADGGHATLQMVALGSLFAVQTVLVFGGFALAAGTLGRAIA
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRPAAGAWLDRIAGLIFIGLALRLATDDHTL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA