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The map label for this gene is lig2 [H]

Identifier: 119896735

GI number: 119896735

Start: 469999

End: 471642

Strand: Direct

Name: lig2 [H]

Synonym: azo0444

Alternate gene names: 119896735

Gene position: 469999-471642 (Clockwise)

Preceding gene: 119896734

Following gene: 119896736

Centisome position: 10.74

GC content: 72.69

Gene sequence:

>1644_bases
ATGAAGGCCTTTGCCGCCCTCTACGCCGAACTCGACGCCACCACTTCCAGCAATGCCAAGCTCGCCGCGATGGTGGCGTA
CTTCCGCGCCGCGCCGGCGGCGGATGCGGCGTGGGCGGCCTACTTCCTCGCCGGCGGCAAACCGCGCCAGCTGGTGCCGG
TGCGCCAGCTGCGGGAATTCGCGATGCGCATCGCCGGCGTGCCCGAATGGCTGTTCGAGGAGAGCTACCAGGCGGTGGGC
GACCTCGCCGAGACGCTGGCGCTGCTGCTGCCCGAAGGCGTGGCGGGAGACGATGCGCCGCTGGCCGAATGGGTGGAGGC
GCGCCTGCTGCCGCTGCGCGGGCTGCCGCCCGACGTGCTGCAGCCGCGGCTGGAGATGCTGTTCGGCGCGCTGTCGACGC
TGCAGCGCTTCGTCTGCGCCAAGCTCATCACCGGCAGCTTCCGCGTCGGCGTGTCGCGCCTGCTCGTCACCCGCGCGCTG
GGCGAGGCCGGCGGGGTCGATACCCGGCGCGTCGCCGAACGCCTGATCGGCTACACCGACATCGGCGCGCGCCCCGACGC
CGCCAGCTACCGCGCGCTGCTCGCGCCGCCCGCGCCGGCCGAGGGCGCGCGCGAAGACGGCCTGCCCTATCCCTTCTTCC
TCGCCCATCCGCTGCAGGCCCCGGTGGAGGCGCTGGAGGACCGCCTCGGGCCGCCGGAAAACTGGCTGATCGAATGGAAA
TGGGACGGCATCCGCGCCCAGCTGGTGCGCCGCGGTGGCCGCAGCTGGCTGTGGTCGCGTGGCGAGGACCTCGTCACCGA
ACGCTTTCCGGAGCTGGCCGCGCTCGGCGCCGAGCTGCCCGAAGGCACCGTGATCGACGGCGAAATCCTGGTGTGGAAGG
CGGGGCGGGTGCAACCCTTCGCCGCGCTGCAGAAGCGGCTCGGGCGCAAGACCGTGGGCACCCGCCTGCTCGCCGAGGCG
CCGGTGGTGCTGTGCGCCTATGACCTGCTCGAGTGGCAGGGCGAAGACCTGCGCGCGCAGCCGCAACACGCCCGCCGCGC
CGCGCTGGAACGGCTGGCGCAGCATCACCCGCAGCGCGCGCTGCAGCTGTCGCCGCTGCTGCACGGGGAGGACTGGGCGG
CGCTCGCGCGCCTGCGCGAGGAGGCCCGCGAGCGCGGCGTCGAAGGCATGATGCTGAAGGGCCGCGACGCGCCCTATGGC
GTGGGCCGCAGCAAGGAAGGCGGCGTGTGGTGGAAATGGAAGATCGACCCCTACAGCATCGACGCGGTGCTGATCTATGC
CCAGCGCGGCCACGGCCGCCGCGCCAGCCTGTACACCGACTACACCTTCGCGGTGTGGGACGCGCCGCCCGACGCGCCGC
AGCGGCGGCTGGTGCCGTTCGCAAAGGCGTATTCCGGCCTGACCGATGCGGAGATCCGGCAGGTGGATGCGATCGTGCGC
AAGACCACCCAGGAGAAGTTCGGCCCGGTGCGCAGCGTGACGCCGGAGCAGGTGTTCGAACTCGGCTTCGAAGGCATCGC
CCGCAGCGCCCGCCACAAGAGCGGCATCGCGGTGCGCTTTCCGCGCATCCTGCGCTGGCGGCACGACAAGCCGGCGGCCG
AGGCCGACACCCTGCAGACACTGGCGGAGCTGCTGCCGCCGTGA

Upstream 100 bases:

>100_bases
CGGCGCAGGCCTTCGCCACCGAATACGGCGAGGAGGACGACGCCGGCCAGGGCGGCGCGGGCGATTCCGCGCGCCCGGAC
GATGCGGCGGAGCCCCCGCA

Downstream 100 bases:

>100_bases
GCACTGCCCGTCCTGTGCCGCTGTCGGCAAACCGCCACGCGAGGGCGGGCCGGTGAGTCCGCCGCGCAAGCCGCCGTCGC
GCCGGGCCACACCCCGGCCG

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MKAFAALYAELDATTSSNAKLAAMVAYFRAAPAADAAWAAYFLAGGKPRQLVPVRQLREFAMRIAGVPEWLFEESYQAVG
DLAETLALLLPEGVAGDDAPLAEWVEARLLPLRGLPPDVLQPRLEMLFGALSTLQRFVCAKLITGSFRVGVSRLLVTRAL
GEAGGVDTRRVAERLIGYTDIGARPDAASYRALLAPPAPAEGAREDGLPYPFFLAHPLQAPVEALEDRLGPPENWLIEWK
WDGIRAQLVRRGGRSWLWSRGEDLVTERFPELAALGAELPEGTVIDGEILVWKAGRVQPFAALQKRLGRKTVGTRLLAEA
PVVLCAYDLLEWQGEDLRAQPQHARRAALERLAQHHPQRALQLSPLLHGEDWAALARLREEARERGVEGMMLKGRDAPYG
VGRSKEGGVWWKWKIDPYSIDAVLIYAQRGHGRRASLYTDYTFAVWDAPPDAPQRRLVPFAKAYSGLTDAEIRQVDAIVR
KTTQEKFGPVRSVTPEQVFELGFEGIARSARHKSGIAVRFPRILRWRHDKPAAEADTLQTLAELLPP

Sequences:

>Translated_547_residues
MKAFAALYAELDATTSSNAKLAAMVAYFRAAPAADAAWAAYFLAGGKPRQLVPVRQLREFAMRIAGVPEWLFEESYQAVG
DLAETLALLLPEGVAGDDAPLAEWVEARLLPLRGLPPDVLQPRLEMLFGALSTLQRFVCAKLITGSFRVGVSRLLVTRAL
GEAGGVDTRRVAERLIGYTDIGARPDAASYRALLAPPAPAEGAREDGLPYPFFLAHPLQAPVEALEDRLGPPENWLIEWK
WDGIRAQLVRRGGRSWLWSRGEDLVTERFPELAALGAELPEGTVIDGEILVWKAGRVQPFAALQKRLGRKTVGTRLLAEA
PVVLCAYDLLEWQGEDLRAQPQHARRAALERLAQHHPQRALQLSPLLHGEDWAALARLREEARERGVEGMMLKGRDAPYG
VGRSKEGGVWWKWKIDPYSIDAVLIYAQRGHGRRASLYTDYTFAVWDAPPDAPQRRLVPFAKAYSGLTDAEIRQVDAIVR
KTTQEKFGPVRSVTPEQVFELGFEGIARSARHKSGIAVRFPRILRWRHDKPAAEADTLQTLAELLPP
>Mature_547_residues
MKAFAALYAELDATTSSNAKLAAMVAYFRAAPAADAAWAAYFLAGGKPRQLVPVRQLREFAMRIAGVPEWLFEESYQAVG
DLAETLALLLPEGVAGDDAPLAEWVEARLLPLRGLPPDVLQPRLEMLFGALSTLQRFVCAKLITGSFRVGVSRLLVTRAL
GEAGGVDTRRVAERLIGYTDIGARPDAASYRALLAPPAPAEGAREDGLPYPFFLAHPLQAPVEALEDRLGPPENWLIEWK
WDGIRAQLVRRGGRSWLWSRGEDLVTERFPELAALGAELPEGTVIDGEILVWKAGRVQPFAALQKRLGRKTVGTRLLAEA
PVVLCAYDLLEWQGEDLRAQPQHARRAALERLAQHHPQRALQLSPLLHGEDWAALARLREEARERGVEGMMLKGRDAPYG
VGRSKEGGVWWKWKIDPYSIDAVLIYAQRGHGRRASLYTDYTFAVWDAPPDAPQRRLVPFAKAYSGLTDAEIRQVDAIVR
KTTQEKFGPVRSVTPEQVFELGFEGIARSARHKSGIAVRFPRILRWRHDKPAAEADTLQTLAELLPP

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=370, Percent_Identity=26.4864864864865, Blast_Score=95, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71985269, Length=456, Percent_Identity=24.780701754386, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320038, Length=372, Percent_Identity=25.5376344086022, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24762424, Length=374, Percent_Identity=28.3422459893048, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI85815841, Length=363, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 60601; Mature: 60601

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFAALYAELDATTSSNAKLAAMVAYFRAAPAADAAWAAYFLAGGKPRQLVPVRQLREF
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
AMRIAGVPEWLFEESYQAVGDLAETLALLLPEGVAGDDAPLAEWVEARLLPLRGLPPDVL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
QPRLEMLFGALSTLQRFVCAKLITGSFRVGVSRLLVTRALGEAGGVDTRRVAERLIGYTD
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
IGARPDAASYRALLAPPAPAEGAREDGLPYPFFLAHPLQAPVEALEDRLGPPENWLIEWK
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCEEEEE
WDGIRAQLVRRGGRSWLWSRGEDLVTERFPELAALGAELPEGTVIDGEILVWKAGRVQPF
ECCHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCHH
AALQKRLGRKTVGTRLLAEAPVVLCAYDLLEWQGEDLRAQPQHARRAALERLAQHHPQRA
HHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
LQLSPLLHGEDWAALARLREEARERGVEGMMLKGRDAPYGVGRSKEGGVWWKWKIDPYSI
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCEEE
DAVLIYAQRGHGRRASLYTDYTFAVWDAPPDAPQRRLVPFAKAYSGLTDAEIRQVDAIVR
EEEEEEEECCCCCEEEEEECEEEEEECCCCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHH
KTTQEKFGPVRSVTPEQVFELGFEGIARSARHKSGIAVRFPRILRWRHDKPAAEADTLQT
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHCCCCCCCHHHHHHH
LAELLPP
HHHHCCC
>Mature Secondary Structure
MKAFAALYAELDATTSSNAKLAAMVAYFRAAPAADAAWAAYFLAGGKPRQLVPVRQLREF
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
AMRIAGVPEWLFEESYQAVGDLAETLALLLPEGVAGDDAPLAEWVEARLLPLRGLPPDVL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
QPRLEMLFGALSTLQRFVCAKLITGSFRVGVSRLLVTRALGEAGGVDTRRVAERLIGYTD
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
IGARPDAASYRALLAPPAPAEGAREDGLPYPFFLAHPLQAPVEALEDRLGPPENWLIEWK
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCEEEEE
WDGIRAQLVRRGGRSWLWSRGEDLVTERFPELAALGAELPEGTVIDGEILVWKAGRVQPF
ECCHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCHH
AALQKRLGRKTVGTRLLAEAPVVLCAYDLLEWQGEDLRAQPQHARRAALERLAQHHPQRA
HHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
LQLSPLLHGEDWAALARLREEARERGVEGMMLKGRDAPYGVGRSKEGGVWWKWKIDPYSI
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCEEE
DAVLIYAQRGHGRRASLYTDYTFAVWDAPPDAPQRRLVPFAKAYSGLTDAEIRQVDAIVR
EEEEEEEECCCCCEEEEEECEEEEEECCCCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHH
KTTQEKFGPVRSVTPEQVFELGFEGIARSARHKSGIAVRFPRILRWRHDKPAAEADTLQT
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHCCCCCCCHHHHHHH
LAELLPP
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA