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The map label for this gene is tolB

Identifier: 119896708

GI number: 119896708

Start: 442120

End: 443403

Strand: Reverse

Name: tolB

Synonym: azo0417

Alternate gene names: 119896708

Gene position: 443403-442120 (Counterclockwise)

Preceding gene: 119896709

Following gene: 119896707

Centisome position: 10.13

GC content: 68.46

Gene sequence:

>1284_bases
ATGAAGACTTTCGCCCAACTCCGTCTGCTCCTCGCCGCCGCCGCACTGGCCTTGCTGTCCTTCTCCGCGCAGGCGCAACT
GTCGATCGAGATCACCGGCGCGGGCGCTTCGCGCTTCCCGGTCATCATCCCGGTATTCGAGAACGAGGCCAGCCTGCCGC
GCAGCGTGTCGGACATCGTGCGCGCCGACCTCGAACGCAGCGGCCTGTTCAGCCTGGTCGACATCGGGCCGCTCCCGCTG
CCTGAAGGACAGATTCCCGATCTCGGCAGCCTGCGCAGCCGCGGCGCCGACGCGGCCCTGGCCGCCAGCGTGTTTCCGCA
GGGCGACGGCCGCTACGAAATCCGCTTCCGCCTGTTCGACACCCAGAAGCAGACCGAACTCGGCGCCCTCGCGCTGCGCA
TGACGGCGGCCCAGAACCGCATCACCGCGCACCGCATCGCCGATTTCGTCTATGAAAAGCTCACCGGCCTGCCCGGCTAC
TTCGCCACCCGCATCGCCTACGTGGTCAAGACCGGGCCGCGCTACGAACTGCAGGTGGCCGACGCCGACGGCATGAATGC
CCAGGCCGCGCTGGTCTCCCGCGAGCCGATCATCTCGCCGGCGTGGTCCCCGGACGGCGGCCGCCTCGCCTACGTCTCGT
TCGAGGCCAAGAAGCCGATCATCTATGTACACACGCTCGCCACCGGCCAGCGCCAGGTGGTGGCCAACTTCAAGGGTTCC
AACTCGGCGCCGGCCTGGTCGCCCGACGGCCAGCAGCTCTCGGTGGTGCTCACCAAGGACGGCCTGTCGCAGCTCTACGT
GCTCAATGCCGACGGCTCCGGCGTGCGCCGCCTGGCAAGCTCCTCCGGCATCGACACCGAGCCGGCCTGGTCGCCCGACG
GCCAGTGGATCTATTTCACTTCCGATCGCGGCGGCAGCCCGCAGATCTACCGCATTCCGACCGCGGGCGGCGCCGCCCAG
CGGGTGACTTTCGACGGCACCTACAACGTGACCGCCCGCCCCTCGGCGGACGGCCGGCTGCTTGCCTTCATCACCCGCAA
CAACGGTCGCTTCCAGGTGGCGGTCCAGGACCTCACCACCCGGCAGACCACGATCCTCACCGATTCGGCGCGTGACGAAT
CGCCCAGCTTCGCACCTAACGGCCGCATGATCCTCTACGCCACCGATGCCGGTGGCCGGGGTGTGCTTGCGGCGGTGTCG
TCCGATGGCCGGGTCAAGCAACGCCTTTCGGTGCAGGCCGCCGATGTACGGGAACCCGCCTGGGGCCCCTTGCAAAAGCA
ATAA

Upstream 100 bases:

>100_bases
GGGGAACTTTTCGAACGAACCCTGGAACTGAAGTTCCGGCCGCTGGAAGAATGATGCGGTCACACGGCTATAATCCACTG
CTTGTGCACCACGTCACGAA

Downstream 100 bases:

>100_bases
CGCTGCTGTCCCCCTAAAAAACGACTTGGAGAAACATCCATGAAGAAGCTTGTCCTGCCCGCTCTGCTGTCCGCCGTTCT
GGCTGCGTGCTCCAGCACCG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKTFAQLRLLLAAAALALLSFSAQAQLSIEITGAGASRFPVIIPVFENEASLPRSVSDIVRADLERSGLFSLVDIGPLPL
PEGQIPDLGSLRSRGADAALAASVFPQGDGRYEIRFRLFDTQKQTELGALALRMTAAQNRITAHRIADFVYEKLTGLPGY
FATRIAYVVKTGPRYELQVADADGMNAQAALVSREPIISPAWSPDGGRLAYVSFEAKKPIIYVHTLATGQRQVVANFKGS
NSAPAWSPDGQQLSVVLTKDGLSQLYVLNADGSGVRRLASSSGIDTEPAWSPDGQWIYFTSDRGGSPQIYRIPTAGGAAQ
RVTFDGTYNVTARPSADGRLLAFITRNNGRFQVAVQDLTTRQTTILTDSARDESPSFAPNGRMILYATDAGGRGVLAAVS
SDGRVKQRLSVQAADVREPAWGPLQKQ

Sequences:

>Translated_427_residues
MKTFAQLRLLLAAAALALLSFSAQAQLSIEITGAGASRFPVIIPVFENEASLPRSVSDIVRADLERSGLFSLVDIGPLPL
PEGQIPDLGSLRSRGADAALAASVFPQGDGRYEIRFRLFDTQKQTELGALALRMTAAQNRITAHRIADFVYEKLTGLPGY
FATRIAYVVKTGPRYELQVADADGMNAQAALVSREPIISPAWSPDGGRLAYVSFEAKKPIIYVHTLATGQRQVVANFKGS
NSAPAWSPDGQQLSVVLTKDGLSQLYVLNADGSGVRRLASSSGIDTEPAWSPDGQWIYFTSDRGGSPQIYRIPTAGGAAQ
RVTFDGTYNVTARPSADGRLLAFITRNNGRFQVAVQDLTTRQTTILTDSARDESPSFAPNGRMILYATDAGGRGVLAAVS
SDGRVKQRLSVQAADVREPAWGPLQKQ
>Mature_427_residues
MKTFAQLRLLLAAAALALLSFSAQAQLSIEITGAGASRFPVIIPVFENEASLPRSVSDIVRADLERSGLFSLVDIGPLPL
PEGQIPDLGSLRSRGADAALAASVFPQGDGRYEIRFRLFDTQKQTELGALALRMTAAQNRITAHRIADFVYEKLTGLPGY
FATRIAYVVKTGPRYELQVADADGMNAQAALVSREPIISPAWSPDGGRLAYVSFEAKKPIIYVHTLATGQRQVVANFKGS
NSAPAWSPDGQQLSVVLTKDGLSQLYVLNADGSGVRRLASSSGIDTEPAWSPDGQWIYFTSDRGGSPQIYRIPTAGGAAQ
RVTFDGTYNVTARPSADGRLLAFITRNNGRFQVAVQDLTTRQTTILTDSARDESPSFAPNGRMILYATDAGGRGVLAAVS
SDGRVKQRLSVQAADVREPAWGPLQKQ

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=424, Percent_Identity=38.4433962264151, Blast_Score=281, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_AZOSB (A1K2H9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931921.1
- ProteinModelPortal:   A1K2H9
- SMR:   A1K2H9
- STRING:   A1K2H9
- GeneID:   4609491
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0417
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   A1K2H9
- ProtClustDB:   PRK02889
- BioCyc:   ASP62928:AZO0417-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 45829; Mature: 45829

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFAQLRLLLAAAALALLSFSAQAQLSIEITGAGASRFPVIIPVFENEASLPRSVSDIV
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHH
RADLERSGLFSLVDIGPLPLPEGQIPDLGSLRSRGADAALAASVFPQGDGRYEIRFRLFD
HHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCEEEEEEEEEC
TQKQTELGALALRMTAAQNRITAHRIADFVYEKLTGLPGYFATRIAYVVKTGPRYELQVA
CCCCCHHHHEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCEEEEEE
DADGMNAQAALVSREPIISPAWSPDGGRLAYVSFEAKKPIIYVHTLATGQRQVVANFKGS
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEEECCCC
NSAPAWSPDGQQLSVVLTKDGLSQLYVLNADGSGVRRLASSSGIDTEPAWSPDGQWIYFT
CCCCCCCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEEEEE
SDRGGSPQIYRIPTAGGAAQRVTFDGTYNVTARPSADGRLLAFITRNNGRFQVAVQDLTT
CCCCCCCEEEEEECCCCCEEEEEECCEEEEEECCCCCCCEEEEEEECCCEEEEEEECCCC
RQTTILTDSARDESPSFAPNGRMILYATDAGGRGVLAAVSSDGRVKQRLSVQAADVREPA
CEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHEEEECCCCCCC
WGPLQKQ
CCCCCCC
>Mature Secondary Structure
MKTFAQLRLLLAAAALALLSFSAQAQLSIEITGAGASRFPVIIPVFENEASLPRSVSDIV
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHH
RADLERSGLFSLVDIGPLPLPEGQIPDLGSLRSRGADAALAASVFPQGDGRYEIRFRLFD
HHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCEEEEEEEEEC
TQKQTELGALALRMTAAQNRITAHRIADFVYEKLTGLPGYFATRIAYVVKTGPRYELQVA
CCCCCHHHHEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCEEEEEE
DADGMNAQAALVSREPIISPAWSPDGGRLAYVSFEAKKPIIYVHTLATGQRQVVANFKGS
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEEECCCC
NSAPAWSPDGQQLSVVLTKDGLSQLYVLNADGSGVRRLASSSGIDTEPAWSPDGQWIYFT
CCCCCCCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEEEEE
SDRGGSPQIYRIPTAGGAAQRVTFDGTYNVTARPSADGRLLAFITRNNGRFQVAVQDLTT
CCCCCCCEEEEEECCCCCEEEEEECCEEEEEECCCCCCCEEEEEEECCCEEEEEEECCCC
RQTTILTDSARDESPSFAPNGRMILYATDAGGRGVLAAVSSDGRVKQRLSVQAADVREPA
CEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHEEEECCCCCCC
WGPLQKQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA