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The map label for this gene is dsbA [H]

Identifier: 119896673

GI number: 119896673

Start: 408982

End: 409629

Strand: Direct

Name: dsbA [H]

Synonym: azo0382

Alternate gene names: 119896673

Gene position: 408982-409629 (Clockwise)

Preceding gene: 119896672

Following gene: 119896674

Centisome position: 9.35

GC content: 68.21

Gene sequence:

>648_bases
ATGAATCGCCGCATCGCCCTCAAGCAGCTCGCCGGTCTTGCCGTCCTCGCCGGCGCCGCCGGTCCCGTCGTCGCCCAGCG
CGCCGCTGCCGCGCCCTTCGTCGAACTCGATACGGTCGTCGCCACCGACAGCAAGGGCAAGGTCGAGGTGATCGAGTTCT
TCCACTACGGCTGCCCGCACTGCCGCGCCTTCGATCCGCTGCTGGAGTCCTGGCTCAAGCGCCTGCCGACCGACGTCGCC
TTCCTCCGCGTGCCGGCGATCTGGGGCAATGCTCAGCTCGGCAAGCTCGCCCAGCTGTATTACGCGATCGAGCTGAGCGG
CAAGGTGGAGCCGTTGCACGGCAAGGTGTTCGTGGCGGTGCAGGACGACAAGGTGCCGCTGCATACCGAAGAGGGGGTGC
GCGAATGGGTCGCCAAGCAGGGCGTGGACACGAAGGCCTTCATGGATGCCTACAAGTCGTTCGGCATGCAGGCGCTGCTC
AAGCAGGCGGACCAGCGCGCGCGCGACTACAAGATCCAGGGCGTGCCGACGATGGCGGTCGACGGCCGCTTCCTGACCTC
GGCGTCGATGACCGGCAGCCACGAGGCGACGCTGAAGGTGGTGGACGATCTCATCGCCCGCGCCCGCAGCCAGCCGCGCC
GCGGTTGA

Upstream 100 bases:

>100_bases
GTCACCAAGTCGGCGCCCTGAAGCGCCTGCAATTCGGCCGCACCGAGGAACGCGGCGCAGCAAACCGCGTCTGAGCCTCG
TCACTGTCCAGGAGTCCCGA

Downstream 100 bases:

>100_bases
TTTCCCGCAGCGTCAGGACTCCGGCGGCATGCTGACGGCCGCCGCCGCAGGGTCTCCACGGGTATTCCTCACCGGCGCCT
CCAGCGGGCTCGGTGCCGCG

Product: putative protein disulfide-isomerase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNRRIALKQLAGLAVLAGAAGPVVAQRAAAAPFVELDTVVATDSKGKVEVIEFFHYGCPHCRAFDPLLESWLKRLPTDVA
FLRVPAIWGNAQLGKLAQLYYAIELSGKVEPLHGKVFVAVQDDKVPLHTEEGVREWVAKQGVDTKAFMDAYKSFGMQALL
KQADQRARDYKIQGVPTMAVDGRFLTSASMTGSHEATLKVVDDLIARARSQPRRG

Sequences:

>Translated_215_residues
MNRRIALKQLAGLAVLAGAAGPVVAQRAAAAPFVELDTVVATDSKGKVEVIEFFHYGCPHCRAFDPLLESWLKRLPTDVA
FLRVPAIWGNAQLGKLAQLYYAIELSGKVEPLHGKVFVAVQDDKVPLHTEEGVREWVAKQGVDTKAFMDAYKSFGMQALL
KQADQRARDYKIQGVPTMAVDGRFLTSASMTGSHEATLKVVDDLIARARSQPRRG
>Mature_215_residues
MNRRIALKQLAGLAVLAGAAGPVVAQRAAAAPFVELDTVVATDSKGKVEVIEFFHYGCPHCRAFDPLLESWLKRLPTDVA
FLRVPAIWGNAQLGKLAQLYYAIELSGKVEPLHGKVFVAVQDDKVPLHTEEGVREWVAKQGVDTKAFMDAYKSFGMQALL
KQADQRARDYKIQGVPTMAVDGRFLTSASMTGSHEATLKVVDDLIARARSQPRRG

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=179, Percent_Identity=29.0502793296089, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23530; Mature: 23530

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRIALKQLAGLAVLAGAAGPVVAQRAAAAPFVELDTVVATDSKGKVEVIEFFHYGCPH
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHCCCCC
CRAFDPLLESWLKRLPTDVAFLRVPAIWGNAQLGKLAQLYYAIELSGKVEPLHGKVFVAV
CHHHHHHHHHHHHHCCCCEEEEEECCEECCCHHHHHHHHHEEEEECCCCCCCCCEEEEEE
QDDKVPLHTEEGVREWVAKQGVDTKAFMDAYKSFGMQALLKQADQRARDYKIQGVPTMAV
ECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEE
DGRFLTSASMTGSHEATLKVVDDLIARARSQPRRG
CCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNRRIALKQLAGLAVLAGAAGPVVAQRAAAAPFVELDTVVATDSKGKVEVIEFFHYGCPH
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHCCCCC
CRAFDPLLESWLKRLPTDVAFLRVPAIWGNAQLGKLAQLYYAIELSGKVEPLHGKVFVAV
CHHHHHHHHHHHHHCCCCEEEEEECCEECCCHHHHHHHHHEEEEECCCCCCCCCEEEEEE
QDDKVPLHTEEGVREWVAKQGVDTKAFMDAYKSFGMQALLKQADQRARDYKIQGVPTMAV
ECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEE
DGRFLTSASMTGSHEATLKVVDDLIARARSQPRRG
CCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10051655 [H]