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The map label for this gene is fixL [H]

Identifier: 119896649

GI number: 119896649

Start: 380934

End: 383189

Strand: Reverse

Name: fixL [H]

Synonym: azo0358

Alternate gene names: 119896649

Gene position: 383189-380934 (Counterclockwise)

Preceding gene: 119896650

Following gene: 119896648

Centisome position: 8.76

GC content: 52.79

Gene sequence:

>2256_bases
ATGTCGAACGGCAGAAATTCATACGAGTCAGCCCTGCGCCCCCGGGCCCGGATCATGAAGACACTTGGGAGCGAACTCAT
CAGCAATGATGCAGTGGCAGTTATCGAATTGGTGAAGAACGCTTATGACGCTGAAGCAAGCCGAGTACTCATCAAATTTG
TCGGACCATTGCAGCCGAAACAAGGTTGTATCGAGATTTTTGACGACGGAGCCGGGATGAGCCTGGATGTCGTCCGGGGG
GCCTGGATGGAGCCCGCAACACCCGGCAAGCGTCAGAAGACCAGTTCTGGATCAAAAGGCCGAAGGGTTCTTGGGGAAAA
AGGCATTGGTCGTTTTGCTGCCATGCGGCTGGCTTCGGAACTGGAGTTGATCACCAGAGCGCGAGGAGCGGACCAAGAGG
TCTATGGGATCTTCGATTGGACGCAATTCGAGGACGAACAGAAGTATCTTGATGAGGTGCTGATCCTTACAGAGGTGCGC
AAGCCTGAAGTCATCCGGAGCGACGTAGGTCTCGATGCCATCTGGCCAAAGCACGAGGTTCCAGTCGAGTGCCCTCCCTC
GGAGCAGGGCACGCTATTGCGGATGAACAATCTTGCGCAGGCCTGGGATGCTGAAAGGTTTCGCCTGATCCAGAGGGGGC
TTTCGCGGCTGATCTCCCCCTTCAAGGAGAACAAGGATTTCAGTGTGTTTCTTCAAGCGCCGGAGGCGTTCTCCGAGTTC
TCATCCGAGATCACGCCTCCCGTGGTCTTGAACTATCCGCATTACACAGTGAGTGGGATGGTCGATCCTCAAGGTAAATG
CGATCTGATGCTTGAGGTGAAAGCTACGGGTGAAGTCAGGAGCGTCACAGGAGGTTTTGTCAGAGAAGGAAAAGATGGCC
TTCAGTATCTTGAGAACGAAGCCTACTCTAAATTGAAGCAGACGACAGAAAACCAGTTAGAAAACGACAAGACTGAATGG
GAAAAGAAACTTCCTACCTGCGGGCCGCTGCAGATTGAATTGCGCATCTGGGATCGGGACGATCTCGGTAACGTCATCCA
ACAAACTAGGTCAAATCTGCAGAACATTCGAGCAGATCTCGATGCCTTCGCAGGCATCAATATATATCGTGACGGATTCA
GGGTCTTGCCTTATGGGGAGCCGAACAACGATTGGCTGCGACTGGATATCCGTCGGGTCCAGAATCCAACAAAGCGCCTG
TCCAACAATCAGATTGTTGGGCACATATCGATTACGGCAGATCAGAACAATGGCTTGAAGGATCAGTCCAATCGCGAAGG
TTTGGACGAAAACCAGAGCTACTCAGACCTAAGGGAGATCATGAAATCGATTCTTTCCAAGATCGAAGATCTTCGAAAGA
GGTCAAAGGGCACAGGTGAAGGCGGCAACAAGGATAAGCAGACCCTTAACCTGTTTGCTCCTCTGGATCTGTCACCTATC
CGGCAGCATTTGGAGTCCGTCAGTCCTAAGGACGAGGTCGCCAAAGAGCTGATCAACAACGTAGAGCGCGCCTTCAACAC
AAAGGTTGAAAGCCTGAAGGCAGTGGTTGCGAAATATCACGCTCTGGCGACATTAGGTCAGCTGATTGACGTTCTACTCC
ATGATGGGCGTCTCCCTCTGGCAAAGATCAGGAAAGAGTCCCTGCTGGCTCAAGAGGACATTGTTGAGGGGCTCCTGACC
GGAGAAGATCTGCTCAACAAGCTGGCTGGCCGTTTGCGCATCATCCATGGGCAGAGTGATGTGCTTGCCACAGTCTTCCG
GCGAGTCGAACCTTTTGGCGGGCGCAAACGGGGACGCCCCAAACAGCTCTATCTTGAGAAGATCATCGAAGATGCATTTG
GCGTGTGCGCAAGCCAGATTGCGGACCTGCAGATCGCAACCTCATTGTCGACCGGACAAACCCTTGTACGGGTAGATGAA
GCTGAAATCCAAGAGGTCATCGTCAATCTGCTGCAGAACAGCCTTTACTGGCTCCAATTTGTTGATACTGCAGTTAGGCG
CATAGATGTCTCTGTGTCGCGCACGGCGCAGGACCACGTTGAGATCATTTTCAGCGACACCGGACCCGGCATTCCGGATG
AGAACAGAGCACGGATCTTTGAACCCTATTTTTCTACGAAGAAGGATGGCGTCGGCCTTGGCTTGGCGATCGTCGGAGAG
ATCATCATGGACTACTACGGCGGGAAGCTTGAGCTGCTGGATTCATCGGGGGCGTCTGGGGCCGTCTTCCGTATCACTTT
AACAAAACGGGTCTGA

Upstream 100 bases:

>100_bases
GTTTGCACCGCACGCAGCAAGACCAACTCTCCTTTTCTGCATTGAGAGACAATTCGGAAAGCAGCAGTTCCTTCCTCCAT
TTTTCATGTGATAACAGCTC

Downstream 100 bases:

>100_bases
CCAGGGGGAAGACATGGATTGGCGGGTTCTGGTCGTTGATGACAAAGCAGCAGATGACGTAGCAGAGACCATCGGAGGGA
ACAAGACAGTCCCAAAGCCA

Product: putative two-component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 751; Mature: 750

Protein sequence:

>751_residues
MSNGRNSYESALRPRARIMKTLGSELISNDAVAVIELVKNAYDAEASRVLIKFVGPLQPKQGCIEIFDDGAGMSLDVVRG
AWMEPATPGKRQKTSSGSKGRRVLGEKGIGRFAAMRLASELELITRARGADQEVYGIFDWTQFEDEQKYLDEVLILTEVR
KPEVIRSDVGLDAIWPKHEVPVECPPSEQGTLLRMNNLAQAWDAERFRLIQRGLSRLISPFKENKDFSVFLQAPEAFSEF
SSEITPPVVLNYPHYTVSGMVDPQGKCDLMLEVKATGEVRSVTGGFVREGKDGLQYLENEAYSKLKQTTENQLENDKTEW
EKKLPTCGPLQIELRIWDRDDLGNVIQQTRSNLQNIRADLDAFAGINIYRDGFRVLPYGEPNNDWLRLDIRRVQNPTKRL
SNNQIVGHISITADQNNGLKDQSNREGLDENQSYSDLREIMKSILSKIEDLRKRSKGTGEGGNKDKQTLNLFAPLDLSPI
RQHLESVSPKDEVAKELINNVERAFNTKVESLKAVVAKYHALATLGQLIDVLLHDGRLPLAKIRKESLLAQEDIVEGLLT
GEDLLNKLAGRLRIIHGQSDVLATVFRRVEPFGGRKRGRPKQLYLEKIIEDAFGVCASQIADLQIATSLSTGQTLVRVDE
AEIQEVIVNLLQNSLYWLQFVDTAVRRIDVSVSRTAQDHVEIIFSDTGPGIPDENRARIFEPYFSTKKDGVGLGLAIVGE
IIMDYYGGKLELLDSSGASGAVFRITLTKRV

Sequences:

>Translated_751_residues
MSNGRNSYESALRPRARIMKTLGSELISNDAVAVIELVKNAYDAEASRVLIKFVGPLQPKQGCIEIFDDGAGMSLDVVRG
AWMEPATPGKRQKTSSGSKGRRVLGEKGIGRFAAMRLASELELITRARGADQEVYGIFDWTQFEDEQKYLDEVLILTEVR
KPEVIRSDVGLDAIWPKHEVPVECPPSEQGTLLRMNNLAQAWDAERFRLIQRGLSRLISPFKENKDFSVFLQAPEAFSEF
SSEITPPVVLNYPHYTVSGMVDPQGKCDLMLEVKATGEVRSVTGGFVREGKDGLQYLENEAYSKLKQTTENQLENDKTEW
EKKLPTCGPLQIELRIWDRDDLGNVIQQTRSNLQNIRADLDAFAGINIYRDGFRVLPYGEPNNDWLRLDIRRVQNPTKRL
SNNQIVGHISITADQNNGLKDQSNREGLDENQSYSDLREIMKSILSKIEDLRKRSKGTGEGGNKDKQTLNLFAPLDLSPI
RQHLESVSPKDEVAKELINNVERAFNTKVESLKAVVAKYHALATLGQLIDVLLHDGRLPLAKIRKESLLAQEDIVEGLLT
GEDLLNKLAGRLRIIHGQSDVLATVFRRVEPFGGRKRGRPKQLYLEKIIEDAFGVCASQIADLQIATSLSTGQTLVRVDE
AEIQEVIVNLLQNSLYWLQFVDTAVRRIDVSVSRTAQDHVEIIFSDTGPGIPDENRARIFEPYFSTKKDGVGLGLAIVGE
IIMDYYGGKLELLDSSGASGAVFRITLTKRV
>Mature_750_residues
SNGRNSYESALRPRARIMKTLGSELISNDAVAVIELVKNAYDAEASRVLIKFVGPLQPKQGCIEIFDDGAGMSLDVVRGA
WMEPATPGKRQKTSSGSKGRRVLGEKGIGRFAAMRLASELELITRARGADQEVYGIFDWTQFEDEQKYLDEVLILTEVRK
PEVIRSDVGLDAIWPKHEVPVECPPSEQGTLLRMNNLAQAWDAERFRLIQRGLSRLISPFKENKDFSVFLQAPEAFSEFS
SEITPPVVLNYPHYTVSGMVDPQGKCDLMLEVKATGEVRSVTGGFVREGKDGLQYLENEAYSKLKQTTENQLENDKTEWE
KKLPTCGPLQIELRIWDRDDLGNVIQQTRSNLQNIRADLDAFAGINIYRDGFRVLPYGEPNNDWLRLDIRRVQNPTKRLS
NNQIVGHISITADQNNGLKDQSNREGLDENQSYSDLREIMKSILSKIEDLRKRSKGTGEGGNKDKQTLNLFAPLDLSPIR
QHLESVSPKDEVAKELINNVERAFNTKVESLKAVVAKYHALATLGQLIDVLLHDGRLPLAKIRKESLLAQEDIVEGLLTG
EDLLNKLAGRLRIIHGQSDVLATVFRRVEPFGGRKRGRPKQLYLEKIIEDAFGVCASQIADLQIATSLSTGQTLVRVDEA
EIQEVIVNLLQNSLYWLQFVDTAVRRIDVSVSRTAQDHVEIIFSDTGPGIPDENRARIFEPYFSTKKDGVGLGLAIVGEI
IMDYYGGKLELLDSSGASGAVFRITLTKRV

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 84018; Mature: 83887

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGRNSYESALRPRARIMKTLGSELISNDAVAVIELVKNAYDAEASRVLIKFVGPLQPK
CCCCCCHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
QGCIEIFDDGAGMSLDVVRGAWMEPATPGKRQKTSSGSKGRRVLGEKGIGRFAAMRLASE
CCHHHEEECCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
LELITRARGADQEVYGIFDWTQFEDEQKYLDEVLILTEVRKPEVIRSDVGLDAIWPKHEV
HHHHHHHCCCCCCEEEEECCHHHCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCC
PVECPPSEQGTLLRMNNLAQAWDAERFRLIQRGLSRLISPFKENKDFSVFLQAPEAFSEF
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
SSEITPPVVLNYPHYTVSGMVDPQGKCDLMLEVKATGEVRSVTGGFVREGKDGLQYLENE
HHCCCCCEEECCCCEEEECCCCCCCCCEEEEEEECCCCHHHHCCHHHHCCHHHHHHHHHH
AYSKLKQTTENQLENDKTEWEKKLPTCGPLQIELRIWDRDDLGNVIQQTRSNLQNIRADL
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
DAFAGINIYRDGFRVLPYGEPNNDWLRLDIRRVQNPTKRLSNNQIVGHISITADQNNGLK
HHHCCCEEECCCCEEECCCCCCCCEEEEEHHHHCCHHHHCCCCEEEEEEEEEECCCCCCC
DQSNREGLDENQSYSDLREIMKSILSKIEDLRKRSKGTGEGGNKDKQTLNLFAPLDLSPI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHH
RQHLESVSPKDEVAKELINNVERAFNTKVESLKAVVAKYHALATLGQLIDVLLHDGRLPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AKIRKESLLAQEDIVEGLLTGEDLLNKLAGRLRIIHGQSDVLATVFRRVEPFGGRKRGRP
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCCCCCCCCH
KQLYLEKIIEDAFGVCASQIADLQIATSLSTGQTLVRVDEAEIQEVIVNLLQNSLYWLQF
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
VDTAVRRIDVSVSRTAQDHVEIIFSDTGPGIPDENRARIFEPYFSTKKDGVGLGLAIVGE
HHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCCCEEECHHHCCCCCCCCHHHHHHHH
IIMDYYGGKLELLDSSGASGAVFRITLTKRV
HHHHHCCCEEEEEECCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SNGRNSYESALRPRARIMKTLGSELISNDAVAVIELVKNAYDAEASRVLIKFVGPLQPK
CCCCCHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
QGCIEIFDDGAGMSLDVVRGAWMEPATPGKRQKTSSGSKGRRVLGEKGIGRFAAMRLASE
CCHHHEEECCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
LELITRARGADQEVYGIFDWTQFEDEQKYLDEVLILTEVRKPEVIRSDVGLDAIWPKHEV
HHHHHHHCCCCCCEEEEECCHHHCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCC
PVECPPSEQGTLLRMNNLAQAWDAERFRLIQRGLSRLISPFKENKDFSVFLQAPEAFSEF
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
SSEITPPVVLNYPHYTVSGMVDPQGKCDLMLEVKATGEVRSVTGGFVREGKDGLQYLENE
HHCCCCCEEECCCCEEEECCCCCCCCCEEEEEEECCCCHHHHCCHHHHCCHHHHHHHHHH
AYSKLKQTTENQLENDKTEWEKKLPTCGPLQIELRIWDRDDLGNVIQQTRSNLQNIRADL
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
DAFAGINIYRDGFRVLPYGEPNNDWLRLDIRRVQNPTKRLSNNQIVGHISITADQNNGLK
HHHCCCEEECCCCEEECCCCCCCCEEEEEHHHHCCHHHHCCCCEEEEEEEEEECCCCCCC
DQSNREGLDENQSYSDLREIMKSILSKIEDLRKRSKGTGEGGNKDKQTLNLFAPLDLSPI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHH
RQHLESVSPKDEVAKELINNVERAFNTKVESLKAVVAKYHALATLGQLIDVLLHDGRLPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AKIRKESLLAQEDIVEGLLTGEDLLNKLAGRLRIIHGQSDVLATVFRRVEPFGGRKRGRP
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCCCCCCCCH
KQLYLEKIIEDAFGVCASQIADLQIATSLSTGQTLVRVDEAEIQEVIVNLLQNSLYWLQF
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
VDTAVRRIDVSVSRTAQDHVEIIFSDTGPGIPDENRARIFEPYFSTKKDGVGLGLAIVGE
HHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCCCEEECHHHCCCCCCCCHHHHHHHH
IIMDYYGGKLELLDSSGASGAVFRITLTKRV
HHHHHCCCEEEEEECCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]