Click here to switch to the map view.

The map label for this gene is vsr [H]

Identifier: 119896647

GI number: 119896647

Start: 379320

End: 379709

Strand: Reverse

Name: vsr [H]

Synonym: azo0356

Alternate gene names: 119896647

Gene position: 379709-379320 (Counterclockwise)

Preceding gene: 119896648

Following gene: 119896646

Centisome position: 8.68

GC content: 61.79

Gene sequence:

>390_bases
ATGATGTCCCGCATCAAGGGACGCGACACCGGACCGGAACTGAGTCTGCGTCGCAATATATGGGCGCTTGGCCTGCGCTA
CCGGTTGCAATACCGGATCGGCCGGACACGCCCCGACATGGTTTTCGTCAGGGCGCGGTTGGCCGTGTTTGTGGATGGTT
GCTTCTGGCACGGTTGTCCGCAGCATTCGACGATGCCGAAGAACAATCGGGATTTCTGGGAGCGGAAGCTCAGGCGCAAC
CGAGAGCGGGACGCGGAGAACACGCATAGCCTCGAGGCAGAGGGCTGGCGAGTGCTGCGTCTCTGGGAGCATGAGATTCA
GGCTTCACCCGCAGACTGCGCACGGCGGATCGCGGTGATGCTGGGGAAGGCGGAGGAATCGAAAGCATGA

Upstream 100 bases:

>100_bases
GGTTCAAGAGCCGCACAATGAAAACGTAGCAAAGAGTCAGATAAAAGGTTAGTCGAGTTTGATGGCTACCAGACTTTTCG
CAACTGAGCAGCGTCGCCAG

Downstream 100 bases:

>100_bases
CCTTCTTCACCGGTGAGACGTTGGGGCAGGTTGACCTGATCGTGGATGCGGTCTACGCGGGCTACAAAACCGAACGCGGT
GGCATGGCCGATCCGCTAGT

Product: very short patch repair endonuclease

Products: DNA [C]

Alternate protein names: V.NaeI [H]

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MMSRIKGRDTGPELSLRRNIWALGLRYRLQYRIGRTRPDMVFVRARLAVFVDGCFWHGCPQHSTMPKNNRDFWERKLRRN
RERDAENTHSLEAEGWRVLRLWEHEIQASPADCARRIAVMLGKAEESKA

Sequences:

>Translated_129_residues
MMSRIKGRDTGPELSLRRNIWALGLRYRLQYRIGRTRPDMVFVRARLAVFVDGCFWHGCPQHSTMPKNNRDFWERKLRRN
RERDAENTHSLEAEGWRVLRLWEHEIQASPADCARRIAVMLGKAEESKA
>Mature_129_residues
MMSRIKGRDTGPELSLRRNIWALGLRYRLQYRIGRTRPDMVFVRARLAVFVDGCFWHGCPQHSTMPKNNRDFWERKLRRN
RERDAENTHSLEAEGWRVLRLWEHEIQASPADCARRIAVMLGKAEESKA

Specific function: May nick NaeI sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=105, Percent_Identity=37.1428571428571, Blast_Score=66, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 15292; Mature: 15292

Theoretical pI: Translated: 11.11; Mature: 11.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSRIKGRDTGPELSLRRNIWALGLRYRLQYRIGRTRPDMVFVRARLAVFVDGCFWHGCP
CCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
QHSTMPKNNRDFWERKLRRNRERDAENTHSLEAEGWRVLRLWEHEIQASPADCARRIAVM
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHH
LGKAEESKA
HCCCCCCCC
>Mature Secondary Structure
MMSRIKGRDTGPELSLRRNIWALGLRYRLQYRIGRTRPDMVFVRARLAVFVDGCFWHGCP
CCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
QHSTMPKNNRDFWERKLRRNRERDAENTHSLEAEGWRVLRLWEHEIQASPADCARRIAVM
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHH
LGKAEESKA
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7698663 [H]