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The map label for this gene is blaA [H]

Identifier: 119896621

GI number: 119896621

Start: 354344

End: 355339

Strand: Reverse

Name: blaA [H]

Synonym: azo0330

Alternate gene names: 119896621

Gene position: 355339-354344 (Counterclockwise)

Preceding gene: 119896623

Following gene: 119896620

Centisome position: 8.12

GC content: 74.3

Gene sequence:

>996_bases
ATGGAAGCTCCAGGAAAACTCCCGCCCCTGCCCGCGCTGCGCAGCTTCGAGGCCGCCGCGCGGCTGCTCAGCTTCAGCCG
CGCCGCCGACGAGCTGCACGTCACCCACGGCGCCGTCAGCCACCAGATCCGCGCGCTCGAAGCGCAGCTCGGACTCGCGC
TGTTCGCGCGCGATACGCGCGGGCTGCGCCTGACGCCCGCCGGCGAGGCGCTGCTGCAGGCCACCAACGGCGCGCTGCGC
GGCATCGCCGACGCCGTCGCGGGGCTGCGCCGCCACCTGCGCCCGGACCGGCTCTCGGTCAGCGTGATGCCGTCCTTCGC
CGGACGCTGGCTGGCGCCGCGCCTGGCGGCCTTTCTCGACGCCAATCCCGGCTGCGAACTCAACGTGCTGTCGTCCGACG
CGATCACCGATTTCGCCCGCGACGGCACCGACCTCGCGATCCGCTGGGGTTTCGGCGGCTACAGCGGGGTGCATAGCGAG
CTGTTGATGGACGACGTGATGTTCCCGGTGGTGAGCCCGCACTTTGCCGGCGGCGTGCCGCGCACCCCGGCCGGGCTGGC
GGGGCTGCCGCTGCTGCGTTCGGTGGGCGAGGACTGGCTGCCGTGGTTCCGCGCCGCCGGGCTGGACTGGCCGGAGCCCT
CGCGCGGGCTGGTGCTGAGCGACTCCGGCCTGCTGGTGCAGGCAGCCATCGACGGCCAGGGCGTGGCGCTGGCGCGCCGC
AGCCTGGCGATGCGCGCCGTGCGCGAAGGCCGCCTGCTGAAACCCTTCGATATCGAGGTGCCGCTGCTTTACGGACCGGG
CCAGGGCAAGCCCCCCTCGCTCGGGCTGGCGCCCGACGGCACGCCCAAGCGCTGGCGGTTCTGGCTGGTGTGGCCGCTCA
CCCCGGCGCCCACACCGCTGTTGCAGCGCTTTGTGGCCTGGCTGCACGCCGAGGTGGCGGCCGAGCTGGACGCCGCCGCG
GCGACCCCCGCCTCAAGCGCGCTCACAGGGCTGTGA

Upstream 100 bases:

>100_bases
ATGACGACACCGCAGGGAAAAACCTTCCGTCTGCGCGTGGGCACCGTCCCGCGCTTTCCGGCGAAAATGCCGTCTCGCTA
CGCTTGCGCCAGCCCCGCCC

Downstream 100 bases:

>100_bases
AAAATGCTCGTTTGATCGCCCGCGCGCCGCGCGCCACCATTGCCCCGTCATCCACTGCCCGCACCCCGTCATGCAACTCG
CCGAACTCAACGTCGCCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MEAPGKLPPLPALRSFEAAARLLSFSRAADELHVTHGAVSHQIRALEAQLGLALFARDTRGLRLTPAGEALLQATNGALR
GIADAVAGLRRHLRPDRLSVSVMPSFAGRWLAPRLAAFLDANPGCELNVLSSDAITDFARDGTDLAIRWGFGGYSGVHSE
LLMDDVMFPVVSPHFAGGVPRTPAGLAGLPLLRSVGEDWLPWFRAAGLDWPEPSRGLVLSDSGLLVQAAIDGQGVALARR
SLAMRAVREGRLLKPFDIEVPLLYGPGQGKPPSLGLAPDGTPKRWRFWLVWPLTPAPTPLLQRFVAWLHAEVAAELDAAA
ATPASSALTGL

Sequences:

>Translated_331_residues
MEAPGKLPPLPALRSFEAAARLLSFSRAADELHVTHGAVSHQIRALEAQLGLALFARDTRGLRLTPAGEALLQATNGALR
GIADAVAGLRRHLRPDRLSVSVMPSFAGRWLAPRLAAFLDANPGCELNVLSSDAITDFARDGTDLAIRWGFGGYSGVHSE
LLMDDVMFPVVSPHFAGGVPRTPAGLAGLPLLRSVGEDWLPWFRAAGLDWPEPSRGLVLSDSGLLVQAAIDGQGVALARR
SLAMRAVREGRLLKPFDIEVPLLYGPGQGKPPSLGLAPDGTPKRWRFWLVWPLTPAPTPLLQRFVAWLHAEVAAELDAAA
ATPASSALTGL
>Mature_331_residues
MEAPGKLPPLPALRSFEAAARLLSFSRAADELHVTHGAVSHQIRALEAQLGLALFARDTRGLRLTPAGEALLQATNGALR
GIADAVAGLRRHLRPDRLSVSVMPSFAGRWLAPRLAAFLDANPGCELNVLSSDAITDFARDGTDLAIRWGFGGYSGVHSE
LLMDDVMFPVVSPHFAGGVPRTPAGLAGLPLLRSVGEDWLPWFRAAGLDWPEPSRGLVLSDSGLLVQAAIDGQGVALARR
SLAMRAVREGRLLKPFDIEVPLLYGPGQGKPPSLGLAPDGTPKRWRFWLVWPLTPAPTPLLQRFVAWLHAEVAAELDAAA
ATPASSALTGL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=313, Percent_Identity=37.3801916932907, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=35.8267716535433, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788706, Length=315, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI145693193, Length=266, Percent_Identity=30.8270676691729, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790262, Length=234, Percent_Identity=29.9145299145299, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI157672245, Length=177, Percent_Identity=36.7231638418079, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786401, Length=249, Percent_Identity=26.1044176706827, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=26.9503546099291, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788887, Length=120, Percent_Identity=35.8333333333333, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35225; Mature: 35225

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAPGKLPPLPALRSFEAAARLLSFSRAADELHVTHGAVSHQIRALEAQLGLALFARDTR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
GLRLTPAGEALLQATNGALRGIADAVAGLRRHLRPDRLSVSVMPSFAGRWLAPRLAAFLD
CEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCHHHHHHHHHHHC
ANPGCELNVLSSDAITDFARDGTDLAIRWGFGGYSGVHSELLMDDVMFPVVSPHFAGGVP
CCCCCEEEEECCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHCCCCC
RTPAGLAGLPLLRSVGEDWLPWFRAAGLDWPEPSRGLVLSDSGLLVQAAIDGQGVALARR
CCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHH
SLAMRAVREGRLLKPFDIEVPLLYGPGQGKPPSLGLAPDGTPKRWRFWLVWPLTPAPTPL
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHH
LQRFVAWLHAEVAAELDAAAATPASSALTGL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MEAPGKLPPLPALRSFEAAARLLSFSRAADELHVTHGAVSHQIRALEAQLGLALFARDTR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
GLRLTPAGEALLQATNGALRGIADAVAGLRRHLRPDRLSVSVMPSFAGRWLAPRLAAFLD
CEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCHHHHHHHHHHHC
ANPGCELNVLSSDAITDFARDGTDLAIRWGFGGYSGVHSELLMDDVMFPVVSPHFAGGVP
CCCCCEEEEECCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHCCCCC
RTPAGLAGLPLLRSVGEDWLPWFRAAGLDWPEPSRGLVLSDSGLLVQAAIDGQGVALARR
CCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHH
SLAMRAVREGRLLKPFDIEVPLLYGPGQGKPPSLGLAPDGTPKRWRFWLVWPLTPAPTPL
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHH
LQRFVAWLHAEVAAELDAAAATPASSALTGL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]