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The map label for this gene is livK1 [C]

Identifier: 119896619

GI number: 119896619

Start: 352603

End: 353745

Strand: Direct

Name: livK1 [C]

Synonym: azo0328

Alternate gene names: 119896619

Gene position: 352603-353745 (Clockwise)

Preceding gene: 119896617

Following gene: 119896622

Centisome position: 8.06

GC content: 71.39

Gene sequence:

>1143_bases
ATGCCCAGCCTGTTACGCCCTTTCCGCCTCGCCGCCGGCCTGCTGGCGCTGCTCGTTCCGCTGGCGCCGTCCGGTGCTGC
CGCGCAGACGCGCGAACTGGTGATCGGCCAGGTGGCGCCGTTTTCCGGACCGCAGGCGGTCACCGGCAAGGCGATCCACG
CCGGCATCCGGCTCTACTTCGACCAGGTGAATGCGCAGGGCGGGGTCAAGGGCGCGCGCCTGCGCCTGGTCACCCGTGAC
GACGCGCAGAAGCCGGAGCAGACGGTTGCGCTCGCCCGCGAGCTGATCCAGCAGGAAGCGCCGGTGGCGCTGATCGGCAC
CGTCGGCACCACCAACGTGGATGCGCTGATCGCCGATGGCGTGCTGGTGCGCGCCGGCCTGCCGCTGGTGGGCGCGGTGT
CCGGCGCGTCGAGCGCGATCGGCGGGCGCAACGTGTTCGTCACCAAGGCCAGCTACCACGATGAGGTGAACCGGCTGTTC
ACCAGCCTTTCCGGCCTCGGCATGAAGCGCGTCGGGCTGGTGTATCAGGACGACGCGCTCGGCCAGGACGTCATCGCCGG
GGCCGACGCCGCGGCGCCGCGCGCCGGCATCAGCCTGATCGCGCGCGCTGGCTACGAGCGCAACACGGTGAAGGTGGAGC
AGGCGGTGGAAGCCATGCTGAAGGCCAATCCGCAGGTGGTCTTCCTCGGCGCCACCACCGCTGCGGCGATCGAGTTCGTG
CGCCAGTACCGCGCGCGCGGCGGCGACGCCACGCTGTACGGGCTGTCGATCATCGACACCCGCCAGCTGGTGGCCCAGCT
CGGCCCCGCCGGTGCGCGCGGCTTTGCGTTCTCGGTGGTGTTGCCGCTGGAAGGCCAGCAGACCATCGAGGTGAACCGCG
AATACCTGCGCCTGCGCGCCGCCAGCACCGACCCCGACCTGTCGGGCCGCTCCATCGAGGGCTTCATCGCCGCCAAGGCA
CTGGTGCACGCGCTGCGCAGCGCCGAGGGCGCGCCGGGCGGCGGCGCGGTGCTGAAGAGCCTGCAGGCGATGCGCAAGGT
CGATCTTGGCGGCTACATGCTGGACTTCGGCCAGCCGGGCCGGCCTGGCTCCAGCTATGTGGACTTCGCCATGCTCGGCG
ATGGCGGCCGGGTGGTGCACTAA

Upstream 100 bases:

>100_bases
GCCGCCACGGCGGCCTGTATTGTGGACAACCGTAATTGCCCGCCGCCGGCCACGCGCCGAACAATCGTCCGCTCGTATTC
CGCCCGCCAGGACGATCGCC

Downstream 100 bases:

>100_bases
GCGCGCCACCCGTCCCCCCGCGAATGGGGCGTCGTGTGTGTGCCGAAAGCCCGCTTACGCTGTCACCGTACCGGGTTTTG
CCGCGCACTCGCGGCCGTCC

Product: putative leucine-specific binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPSLLRPFRLAAGLLALLVPLAPSGAAAQTRELVIGQVAPFSGPQAVTGKAIHAGIRLYFDQVNAQGGVKGARLRLVTRD
DAQKPEQTVALARELIQQEAPVALIGTVGTTNVDALIADGVLVRAGLPLVGAVSGASSAIGGRNVFVTKASYHDEVNRLF
TSLSGLGMKRVGLVYQDDALGQDVIAGADAAAPRAGISLIARAGYERNTVKVEQAVEAMLKANPQVVFLGATTAAAIEFV
RQYRARGGDATLYGLSIIDTRQLVAQLGPAGARGFAFSVVLPLEGQQTIEVNREYLRLRAASTDPDLSGRSIEGFIAAKA
LVHALRSAEGAPGGGAVLKSLQAMRKVDLGGYMLDFGQPGRPGSSYVDFAMLGDGGRVVH

Sequences:

>Translated_380_residues
MPSLLRPFRLAAGLLALLVPLAPSGAAAQTRELVIGQVAPFSGPQAVTGKAIHAGIRLYFDQVNAQGGVKGARLRLVTRD
DAQKPEQTVALARELIQQEAPVALIGTVGTTNVDALIADGVLVRAGLPLVGAVSGASSAIGGRNVFVTKASYHDEVNRLF
TSLSGLGMKRVGLVYQDDALGQDVIAGADAAAPRAGISLIARAGYERNTVKVEQAVEAMLKANPQVVFLGATTAAAIEFV
RQYRARGGDATLYGLSIIDTRQLVAQLGPAGARGFAFSVVLPLEGQQTIEVNREYLRLRAASTDPDLSGRSIEGFIAAKA
LVHALRSAEGAPGGGAVLKSLQAMRKVDLGGYMLDFGQPGRPGSSYVDFAMLGDGGRVVH
>Mature_379_residues
PSLLRPFRLAAGLLALLVPLAPSGAAAQTRELVIGQVAPFSGPQAVTGKAIHAGIRLYFDQVNAQGGVKGARLRLVTRDD
AQKPEQTVALARELIQQEAPVALIGTVGTTNVDALIADGVLVRAGLPLVGAVSGASSAIGGRNVFVTKASYHDEVNRLFT
SLSGLGMKRVGLVYQDDALGQDVIAGADAAAPRAGISLIARAGYERNTVKVEQAVEAMLKANPQVVFLGATTAAAIEFVR
QYRARGGDATLYGLSIIDTRQLVAQLGPAGARGFAFSVVLPLEGQQTIEVNREYLRLRAASTDPDLSGRSIEGFIAAKAL
VHALRSAEGAPGGGAVLKSLQAMRKVDLGGYMLDFGQPGRPGSSYVDFAMLGDGGRVVH

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39499; Mature: 39368

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLLRPFRLAAGLLALLVPLAPSGAAAQTRELVIGQVAPFSGPQAVTGKAIHAGIRLYF
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHEEE
DQVNAQGGVKGARLRLVTRDDAQKPEQTVALARELIQQEAPVALIGTVGTTNVDALIADG
EHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCC
VLVRAGLPLVGAVSGASSAIGGRNVFVTKASYHDEVNRLFTSLSGLGMKRVGLVYQDDAL
EEEECCCCCEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCHHHCCEEEECCCC
GQDVIAGADAAAPRAGISLIARAGYERNTVKVEQAVEAMLKANPQVVFLGATTAAAIEFV
CCHHHCCCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
RQYRARGGDATLYGLSIIDTRQLVAQLGPAGARGFAFSVVLPLEGQQTIEVNREYLRLRA
HHHHHCCCCEEEEEEEEHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHCHHHHEEEE
ASTDPDLSGRSIEGFIAAKALVHALRSAEGAPGGGAVLKSLQAMRKVDLGGYMLDFGQPG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
RPGSSYVDFAMLGDGGRVVH
CCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
PSLLRPFRLAAGLLALLVPLAPSGAAAQTRELVIGQVAPFSGPQAVTGKAIHAGIRLYF
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHEEE
DQVNAQGGVKGARLRLVTRDDAQKPEQTVALARELIQQEAPVALIGTVGTTNVDALIADG
EHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCC
VLVRAGLPLVGAVSGASSAIGGRNVFVTKASYHDEVNRLFTSLSGLGMKRVGLVYQDDAL
EEEECCCCCEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCHHHCCEEEECCCC
GQDVIAGADAAAPRAGISLIARAGYERNTVKVEQAVEAMLKANPQVVFLGATTAAAIEFV
CCHHHCCCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
RQYRARGGDATLYGLSIIDTRQLVAQLGPAGARGFAFSVVLPLEGQQTIEVNREYLRLRA
HHHHHCCCCEEEEEEEEHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHCHHHHEEEE
ASTDPDLSGRSIEGFIAAKALVHALRSAEGAPGGGAVLKSLQAMRKVDLGGYMLDFGQPG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
RPGSSYVDFAMLGDGGRVVH
CCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA