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The map label for this gene is paaJ1 [H]

Identifier: 119896601

GI number: 119896601

Start: 333560

End: 334762

Strand: Direct

Name: paaJ1 [H]

Synonym: azo0310

Alternate gene names: 119896601

Gene position: 333560-334762 (Clockwise)

Preceding gene: 119896600

Following gene: 119896602

Centisome position: 7.62

GC content: 70.91

Gene sequence:

>1203_bases
GTGACCGAAGTCTTCATCTGCGACGGCATCCGCACCCCGATCGGCCGCTACGGCGGCGCGCTGTCCGGCGTGCGCACCGA
CGATCTCGCCGCGCTGCCGATCAAGGCCCTGATCGCCCGCAACAGCGGCGTGGATTGGGCGGCCGTCGACGACATCTACT
ACGGCTGCGCCAACCAGGCCGGCGAAGACAACCGCGACGTCGCGCGCATGGCCGGCCTGCTCGCCGGGCTGCCGATCGAC
GTGCCCGGCTCCACGATCAACCGCCTGTGCGGCTCGGGCATGGACGCGGTGGGCACCGCCGCGCGCGCGATCCGCACCGG
CGAGACCCAGCTGATGATCGCCGGCGGCGTCGAGAGCATGAGCCGCGCGCCCTTCGTCATGGGCAAGGCCGATACCGCGT
TCTCGCGCAGCGCGAAGATCGAGGACACCACGATCGGCTGGCGCTTCGTCAACCCGCTGATGAAGGCCAAGTACGGCATC
GACTCGATGCCCGAGACCGCCGAGAACGTGGCCACCGATTTCAACATCAACCGCGCCGATCAGGACGCCTTCGCGCTGCG
CAGCCAGCTGCGCTACGCCGCCGCGGCCGAGCGCGGCTTCTACGAGGGCGAACTGGTGCCGGTGGAGATTGCGCAGAAGA
AGGGCGATCCGCTGCGCGTCACCGCCGACGAGCATCCGCGTGTGACCACGCTGGAGGCGCTCGCCAAGCTGAAGGGCGTG
GTGCGCCCGGACGGTACCGTCACCGCCGGCAACGCGTCCGGCGTCAATGACGGCTCCGTGGCGCTGCTGCTTGCCTCCGA
AGCCGCCGCCGCGAAGCACGGCCTCAAGCCGCGCGCCCGCATCGTCGCGATGGCCACCGCGGGCGTCGAGCCGCGCATCA
TGGGCATCGGCCCGGCGCCGGCCTCGCGCAAGCTGCTGGCACTGACCGGCCTCAGCCTCGACCAGATGGACGTCATCGAA
CTCAACGAGGCCTTCGCCGCGCAGGCGCTGGCGGTGACCCGCCAGCTCGGCCTCGCCGACGACGACGCGCGCGTCAATCC
CAACGGCGGTGCAATCTCGCTCGGTCATCCTCTCGGCGCCTCGGGCGCGCGCCTGGTGCTCACCGCGCTGCGCCAGCTCG
AAGCCACCGGCGGCCGCTACGGCCTGTGCACCATGTGCATCGGCGTCGGCCAGGGCATCGCGATGATCATCGAACGCGTC
TGA

Upstream 100 bases:

>100_bases
GCGCAGCTGCCGGCGCGCGGCCGCAAGCCGCCGGCCGAGGCCGATGCCGCCTGATTCCGTTCCAACCCCGCACATAGAGA
AAACACAAGGAGAACAGCCC

Downstream 100 bases:

>100_bases
ACACATCTTTCACCGGCAGTGGAGTTTGATATTTTCAAATGAATTAGTGATGTGATAATTTCCCTGCGCCGTCCGGACAA
CAACATTCAAGCCGGCGGCG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTEVFICDGIRTPIGRYGGALSGVRTDDLAALPIKALIARNSGVDWAAVDDIYYGCANQAGEDNRDVARMAGLLAGLPID
VPGSTINRLCGSGMDAVGTAARAIRTGETQLMIAGGVESMSRAPFVMGKADTAFSRSAKIEDTTIGWRFVNPLMKAKYGI
DSMPETAENVATDFNINRADQDAFALRSQLRYAAAAERGFYEGELVPVEIAQKKGDPLRVTADEHPRVTTLEALAKLKGV
VRPDGTVTAGNASGVNDGSVALLLASEAAAAKHGLKPRARIVAMATAGVEPRIMGIGPAPASRKLLALTGLSLDQMDVIE
LNEAFAAQALAVTRQLGLADDDARVNPNGGAISLGHPLGASGARLVLTALRQLEATGGRYGLCTMCIGVGQGIAMIIERV

Sequences:

>Translated_400_residues
MTEVFICDGIRTPIGRYGGALSGVRTDDLAALPIKALIARNSGVDWAAVDDIYYGCANQAGEDNRDVARMAGLLAGLPID
VPGSTINRLCGSGMDAVGTAARAIRTGETQLMIAGGVESMSRAPFVMGKADTAFSRSAKIEDTTIGWRFVNPLMKAKYGI
DSMPETAENVATDFNINRADQDAFALRSQLRYAAAAERGFYEGELVPVEIAQKKGDPLRVTADEHPRVTTLEALAKLKGV
VRPDGTVTAGNASGVNDGSVALLLASEAAAAKHGLKPRARIVAMATAGVEPRIMGIGPAPASRKLLALTGLSLDQMDVIE
LNEAFAAQALAVTRQLGLADDDARVNPNGGAISLGHPLGASGARLVLTALRQLEATGGRYGLCTMCIGVGQGIAMIIERV
>Mature_399_residues
TEVFICDGIRTPIGRYGGALSGVRTDDLAALPIKALIARNSGVDWAAVDDIYYGCANQAGEDNRDVARMAGLLAGLPIDV
PGSTINRLCGSGMDAVGTAARAIRTGETQLMIAGGVESMSRAPFVMGKADTAFSRSAKIEDTTIGWRFVNPLMKAKYGID
SMPETAENVATDFNINRADQDAFALRSQLRYAAAAERGFYEGELVPVEIAQKKGDPLRVTADEHPRVTTLEALAKLKGVV
RPDGTVTAGNASGVNDGSVALLLASEAAAAKHGLKPRARIVAMATAGVEPRIMGIGPAPASRKLLALTGLSLDQMDVIEL
NEAFAAQALAVTRQLGLADDDARVNPNGGAISLGHPLGASGARLVLTALRQLEATGGRYGLCTMCIGVGQGIAMIIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=43.21608040201, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI148539872, Length=405, Percent_Identity=40.2469135802469, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI4557237, Length=404, Percent_Identity=39.6039603960396, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI4501853, Length=403, Percent_Identity=40.1985111662531, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=32.7868852459016, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI194353979, Length=398, Percent_Identity=29.1457286432161, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=67.8304239401496, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=44.6913580246914, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.409429280397, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=43.25, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI1788683, Length=425, Percent_Identity=30.5882352941176, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=43.3915211970075, Blast_Score=305, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI25147385, Length=402, Percent_Identity=34.5771144278607, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=33.2512315270936, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=32.7906976744186, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=30.5210918114144, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=35.2357320099256, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6322031, Length=412, Percent_Identity=36.4077669902913, Blast_Score=210, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=44.139650872818, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI17648125, Length=401, Percent_Identity=38.9027431421446, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=35.8910891089109, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=32.7868852459016, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI19921506, Length=309, Percent_Identity=28.1553398058252, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585051, Length=309, Percent_Identity=28.1553398058252, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41686; Mature: 41555

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVFICDGIRTPIGRYGGALSGVRTDDLAALPIKALIARNSGVDWAAVDDIYYGCANQA
CCEEEEECCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCC
GEDNRDVARMAGLLAGLPIDVPGSTINRLCGSGMDAVGTAARAIRTGETQLMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCHHHH
SRAPFVMGKADTAFSRSAKIEDTTIGWRFVNPLMKAKYGIDSMPETAENVATDFNINRAD
CCCCEEEECCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
QDAFALRSQLRYAAAAERGFYEGELVPVEIAQKKGDPLRVTADEHPRVTTLEALAKLKGV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGSVALLLASEAAAAKHGLKPRARIVAMATAGVEPRIMGIGPAP
CCCCCCEEECCCCCCCCCCEEEEEECHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCC
ASRKLLALTGLSLDQMDVIELNEAFAAQALAVTRQLGLADDDARVNPNGGAISLGHPLGA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCC
SGARLVLTALRQLEATGGRYGLCTMCIGVGQGIAMIIERV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
TEVFICDGIRTPIGRYGGALSGVRTDDLAALPIKALIARNSGVDWAAVDDIYYGCANQA
CEEEEECCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCC
GEDNRDVARMAGLLAGLPIDVPGSTINRLCGSGMDAVGTAARAIRTGETQLMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCHHHH
SRAPFVMGKADTAFSRSAKIEDTTIGWRFVNPLMKAKYGIDSMPETAENVATDFNINRAD
CCCCEEEECCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
QDAFALRSQLRYAAAAERGFYEGELVPVEIAQKKGDPLRVTADEHPRVTTLEALAKLKGV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGSVALLLASEAAAAKHGLKPRARIVAMATAGVEPRIMGIGPAP
CCCCCCEEECCCCCCCCCCEEEEEECHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCC
ASRKLLALTGLSLDQMDVIELNEAFAAQALAVTRQLGLADDDARVNPNGGAISLGHPLGA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCC
SGARLVLTALRQLEATGGRYGLCTMCIGVGQGIAMIIERV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125318 [H]