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The map label for this gene is gatA

Identifier: 119896464

GI number: 119896464

Start: 188336

End: 189799

Strand: Reverse

Name: gatA

Synonym: azo0172

Alternate gene names: 119896464

Gene position: 189799-188336 (Counterclockwise)

Preceding gene: 119896465

Following gene: 119896463

Centisome position: 4.34

GC content: 70.7

Gene sequence:

>1464_bases
ATGATCAACGCTTCCGTTTCCGAGCTGCGCCGCGCACTCGACACCCGGCAGGTTTCCAGCGTCGAGCTGGCCACGCTCTT
CCTCGACCGCATCGCGGAACGCAATCCGGCCCTCAACGCCTTCATCACGATCGACCGCGAGGGCGCGCTCGCCGCCGCGC
GCGCGGCCGACGCCCGCATCGCCGCCGGCACTGCCGGTCCGCTCACCGGCATCCCGCTCGCGCACAAGGACCTGTTCTGC
ACCGAAGGCGTGCTGACCACCTGTGGCTCGAAGATGCTGGCCGACTTCGTCAGCCCCTATGACGCCCACGTCGTCAGCCG
GCTGAAGGACGCCGGCGCCGTCAGCCTGGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGTCGAACGAGTCCTCGC
ACTACGGCGCGGTGCGCAATCCGTGGGACACCACCCGCATTCCCGGCGGCTCGTCGGGCGGCTCGGCGGCCGCGGTGGCC
GCCCGCCTGGTGCCGCTCGCCACTGGCTCCGACACCGGCGGCTCGGTGCGCCAGCCCGCCTCGCACACCGGCGTCACCGG
TATCAAGCCCACCTACGGCGTCGTCAGCCGCTACGGCATGATCGCCTACGCCTCCTCGCTCGATCAGGGCGGCGCCTTCG
GCGCTTCGGCGGAAGACTGCGCGCTGCTGCTCACGGCCATGGCCGGCTTCGATCCGCGCGACTCCACCTGCCTCGACCGC
CCGGCCGAGGACTACGCGGCCGCACTCGCGCCCACCGCCGGCGGCAAGCCGCTCGCCGGCCTGCGCATCGGCCTGCCGCG
CGAGTTCTTCGCCGAAGGCATGGCGGACGACGTGCGCGCAGCGGTGGACGCCGCGCTCGACCAGTACCGCGCGCTCGGCG
CCGTCACCGTCGAGGTCAGCCTGCCCAACGCCAAGCTGGCCGTGCCCGCCTACTACGTGATCGCGCCGGCCGAGGCGTCG
AGCAACCTGTCCCGTTTCGACGGCGTGCGCTACGGCCACCGCGCAGCGGAATACGGCGACCTCAACGACATGTACTGCAA
GAGCCGCGCGGAAGGCTTCGGCGCCGAGGTCAAGCGCCGCATCCTGGTCGGCACCTATGTGCTGTCGCACGGCTACTACG
ACGCCTACTACCTGCAGGCGCAGAAGCTGCGCCGCCTGATCGCGCAGGACTTCCAGGCCGCCTTCGCGCAGTGCGACGTC
ATCGCCGGCCCCACCAGCCCGACCACAGCGTGGGCGATTGGCGAGAAGGCGGACGACCCGGTGCAGATGTACCTGTCGGA
CATCTACACGATCGCGGTCAACCTCGCCGGCTTGCCCGGCCTGTCCCACCCCTGCGGTTTCGGCGCCGGGCGCCTGCCGG
TGGGCCTGCAGCTGATCGGCAACTACTTCGGCGAAAGCCGCCTGCTGGCCACTGCACACCAGTACCAGCAGGCAAGCGAC
TGGCATCTGCAGCGCCCCGAGTAA

Upstream 100 bases:

>100_bases
CCGCAGACCGAGGCCGGCCTCTATCTGGTGCCCAAGGTCATCGAATGAACCCGCGGCCGGCACCCCTGTGCTGCCGGCCG
CGCCCACACGCTTCCCGCCC

Downstream 100 bases:

>100_bases
AGCCCATGGCCCGCTTCGCCCTCCGCCTCGCCGGCCGCATCGCCGCAGCCGAGTTCGCGCTGTGGCTGGCGGCCTGCGCG
ACGCCCCCGGCCGGAACCGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MINASVSELRRALDTRQVSSVELATLFLDRIAERNPALNAFITIDREGALAAARAADARIAAGTAGPLTGIPLAHKDLFC
TEGVLTTCGSKMLADFVSPYDAHVVSRLKDAGAVSLGKTNMDEFAMGSSNESSHYGAVRNPWDTTRIPGGSSGGSAAAVA
ARLVPLATGSDTGGSVRQPASHTGVTGIKPTYGVVSRYGMIAYASSLDQGGAFGASAEDCALLLTAMAGFDPRDSTCLDR
PAEDYAAALAPTAGGKPLAGLRIGLPREFFAEGMADDVRAAVDAALDQYRALGAVTVEVSLPNAKLAVPAYYVIAPAEAS
SNLSRFDGVRYGHRAAEYGDLNDMYCKSRAEGFGAEVKRRILVGTYVLSHGYYDAYYLQAQKLRRLIAQDFQAAFAQCDV
IAGPTSPTTAWAIGEKADDPVQMYLSDIYTIAVNLAGLPGLSHPCGFGAGRLPVGLQLIGNYFGESRLLATAHQYQQASD
WHLQRPE

Sequences:

>Translated_487_residues
MINASVSELRRALDTRQVSSVELATLFLDRIAERNPALNAFITIDREGALAAARAADARIAAGTAGPLTGIPLAHKDLFC
TEGVLTTCGSKMLADFVSPYDAHVVSRLKDAGAVSLGKTNMDEFAMGSSNESSHYGAVRNPWDTTRIPGGSSGGSAAAVA
ARLVPLATGSDTGGSVRQPASHTGVTGIKPTYGVVSRYGMIAYASSLDQGGAFGASAEDCALLLTAMAGFDPRDSTCLDR
PAEDYAAALAPTAGGKPLAGLRIGLPREFFAEGMADDVRAAVDAALDQYRALGAVTVEVSLPNAKLAVPAYYVIAPAEAS
SNLSRFDGVRYGHRAAEYGDLNDMYCKSRAEGFGAEVKRRILVGTYVLSHGYYDAYYLQAQKLRRLIAQDFQAAFAQCDV
IAGPTSPTTAWAIGEKADDPVQMYLSDIYTIAVNLAGLPGLSHPCGFGAGRLPVGLQLIGNYFGESRLLATAHQYQQASD
WHLQRPE
>Mature_487_residues
MINASVSELRRALDTRQVSSVELATLFLDRIAERNPALNAFITIDREGALAAARAADARIAAGTAGPLTGIPLAHKDLFC
TEGVLTTCGSKMLADFVSPYDAHVVSRLKDAGAVSLGKTNMDEFAMGSSNESSHYGAVRNPWDTTRIPGGSSGGSAAAVA
ARLVPLATGSDTGGSVRQPASHTGVTGIKPTYGVVSRYGMIAYASSLDQGGAFGASAEDCALLLTAMAGFDPRDSTCLDR
PAEDYAAALAPTAGGKPLAGLRIGLPREFFAEGMADDVRAAVDAALDQYRALGAVTVEVSLPNAKLAVPAYYVIAPAEAS
SNLSRFDGVRYGHRAAEYGDLNDMYCKSRAEGFGAEVKRRILVGTYVLSHGYYDAYYLQAQKLRRLIAQDFQAAFAQCDV
IAGPTSPTTAWAIGEKADDPVQMYLSDIYTIAVNLAGLPGLSHPCGFGAGRLPVGLQLIGNYFGESRLLATAHQYQQASD
WHLQRPE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=37.9241516966068, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=25.8196721311475, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17543272, Length=416, Percent_Identity=38.7019230769231, Blast_Score=281, Evalue=7e-76,
Organism=Caenorhabditis elegans, GI17537465, Length=510, Percent_Identity=25.4901960784314, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=231, Percent_Identity=30.3030303030303, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=183, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=183, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=193, Percent_Identity=31.0880829015544, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=187, Percent_Identity=32.620320855615, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=197, Percent_Identity=29.9492385786802, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=429, Percent_Identity=37.7622377622378, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=484, Percent_Identity=28.3057851239669, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=197, Percent_Identity=31.9796954314721, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648113, Length=456, Percent_Identity=42.7631578947368, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24652985, Length=500, Percent_Identity=28.2, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19922090, Length=500, Percent_Identity=28.2, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24652981, Length=500, Percent_Identity=28.2, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24652983, Length=500, Percent_Identity=28.2, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI161078093, Length=492, Percent_Identity=28.4552845528455, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550774, Length=493, Percent_Identity=24.7464503042596, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648435, Length=493, Percent_Identity=24.7464503042596, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648437, Length=493, Percent_Identity=24.7464503042596, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24644968, Length=518, Percent_Identity=25.0965250965251, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356731, Length=205, Percent_Identity=32.1951219512195, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24648441, Length=428, Percent_Identity=24.5327102803738, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648439, Length=428, Percent_Identity=24.5327102803738, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_AZOSB (A1K1T5)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931677.1
- ProteinModelPortal:   A1K1T5
- SMR:   A1K1T5
- STRING:   A1K1T5
- GeneID:   4606249
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0172
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   A1K1T5
- ProtClustDB:   PRK00012
- BioCyc:   ASP62928:AZO0172-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51288; Mature: 51288

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 151-151 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINASVSELRRALDTRQVSSVELATLFLDRIAERNPALNAFITIDREGALAAARAADARI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCEE
AAGTAGPLTGIPLAHKDLFCTEGVLTTCGSKMLADFVSPYDAHVVSRLKDAGAVSLGKTN
ECCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCC
MDEFAMGSSNESSHYGAVRNPWDTTRIPGGSSGGSAAAVAARLVPLATGSDTGGSVRQPA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCCC
SHTGVTGIKPTYGVVSRYGMIAYASSLDQGGAFGASAEDCALLLTAMAGFDPRDSTCLDR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PAEDYAAALAPTAGGKPLAGLRIGLPREFFAEGMADDVRAAVDAALDQYRALGAVTVEVS
CHHHHHHHHCCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEE
LPNAKLAVPAYYVIAPAEASSNLSRFDGVRYGHRAAEYGDLNDMYCKSRAEGFGAEVKRR
CCCCEEECCEEEEEECCCCCCCHHHHCCCHHCCHHHCCCCCHHHHHHHHHCCCCHHHHHH
ILVGTYVLSHGYYDAYYLQAQKLRRLIAQDFQAAFAQCDVIAGPTSPTTAWAIGEKADDP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCEEECCCCCCCH
VQMYLSDIYTIAVNLAGLPGLSHPCGFGAGRLPVGLQLIGNYFGESRLLATAHQYQQASD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCC
WHLQRPE
CCCCCCC
>Mature Secondary Structure
MINASVSELRRALDTRQVSSVELATLFLDRIAERNPALNAFITIDREGALAAARAADARI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCEE
AAGTAGPLTGIPLAHKDLFCTEGVLTTCGSKMLADFVSPYDAHVVSRLKDAGAVSLGKTN
ECCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCC
MDEFAMGSSNESSHYGAVRNPWDTTRIPGGSSGGSAAAVAARLVPLATGSDTGGSVRQPA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCCC
SHTGVTGIKPTYGVVSRYGMIAYASSLDQGGAFGASAEDCALLLTAMAGFDPRDSTCLDR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PAEDYAAALAPTAGGKPLAGLRIGLPREFFAEGMADDVRAAVDAALDQYRALGAVTVEVS
CHHHHHHHHCCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEE
LPNAKLAVPAYYVIAPAEASSNLSRFDGVRYGHRAAEYGDLNDMYCKSRAEGFGAEVKRR
CCCCEEECCEEEEEECCCCCCCHHHHCCCHHCCHHHCCCCCHHHHHHHHHCCCCHHHHHH
ILVGTYVLSHGYYDAYYLQAQKLRRLIAQDFQAAFAQCDVIAGPTSPTTAWAIGEKADDP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCEEECCCCCCCH
VQMYLSDIYTIAVNLAGLPGLSHPCGFGAGRLPVGLQLIGNYFGESRLLATAHQYQQASD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCC
WHLQRPE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA