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The map label for this gene is gatB

Identifier: 119896462

GI number: 119896462

Start: 186300

End: 187760

Strand: Reverse

Name: gatB

Synonym: azo0170

Alternate gene names: 119896462

Gene position: 187760-186300 (Counterclockwise)

Preceding gene: 119896463

Following gene: 119896461

Centisome position: 4.29

GC content: 65.91

Gene sequence:

>1461_bases
ATGAGCAGATCGGATTGGGAAGTCGTCATCGGGCTGGAGGTCCATGCCCAGCTCAACACCGCGTCGAAGATTTTCTCCGG
CGCCAGCACCGCCTTCGGCGCGGAACCCAACGTCCAGGCGAGCGCGGTCGATATCGCGCTGCCGGGCGTGCTGCCGGTGC
TCAACCGCGGCGCGGTCGAACGCGCGATCCGCTTCGGCCTCGCGATCGGCGCCACGGTGGCGCCGACCAGCGTGTTCGCG
CGCAAGAACTACTTCTACCCCGACCTCCCCAAGGGTTACCAGATCAGCCAGTTCGAGCTGCCGGTGGTGCAGGGCGGCAC
CATCACCATCCGCGTCGGCGAAGGTGAGAACGCCTACGAGAAGACGGTGAACCTGACCCGCGCCCATCTGGAAGAAGACG
CCGGCAAGAGCCTGCACGAAGATTTCCACGGCATGAGCGGCATCGACCTCAACCGCGCCGGCACGCCGCTGCTCGAGATC
GTCTCCGAGCCCGACATGCGCTCGTCGGCGGAAGCCGTGGCCTACGCCCGCACGCTGCATGCGCTGGTGCGCTGGATCGA
CATCTGCGACGGCAACATGCAGGAAGGCTCGTTCCGCTGCGACGCCAACGTCTCGGTGCGCAAGAAGGGCGCGGAGAAGT
TCGGCACCCGGCGCGAGATCAAGAACCTCAACTCCTTCCGCTTCCTGCAGCAGGCGATCGACTACGAGGTGCAGTGGCAG
ATCGACACCATCGAGGACGGCGGCACCATCCAGCAGGCCACCGTGCTGTTCGATCCGGACACCGGCGAGACGCGAATGAT
GCGCTCCAAGGAAGACGCCCACGACTACCGCTACTTCCCCGACCCGGACCTGCTGCCGCTGGTGATTTCGCCGGAATGGA
AGGCGCGGGTGCAGGGCGAGATGCCGGAACTGCCGGAAGCGATGAAGGCCCGCTTCATCGAGCAGCTCGGCCTCTCTGCC
TACGACGCCACCACGCTCACCGCGTCCAAGGAAGTCGCCACGTATTACCAGGCCACCGTGGACGCCGCCGGCGCCGCGCT
CGCCAAGCCCTGCGCCAACTGGGTGATGGGCGACCTCGCCGCGCGCCTCAACAAGGCCGAACTCGACATCGCCGTGTCGC
CGGTATCGCCCGCCCAGCTCGCCGGCCTGGTGGCGCGCATCGCCGACAACACCATCTCCAACGCGATCGCCAAGAAGGTG
TTCGAGGCGTTGTGGAACGGCGAAGGCGCCAGTGCTGACGAAATCATCGACAAGCAGGGCCTCAAGCAGGTCACCGACAG
CGGCGCGATCGAAGCGATGATCGACGAAGTGCTCGCCGCCAACCAGAAGTCGGTGGAGGAATTCCGCGCCGGCAAGGACA
AGGCTTTCAATGCGCTGGTGGGCCAGGTCATGAAGGCGAGCAAGGGCAAGGCCAGCCCGGCCCAGGTCAACGAACTGCTG
AAGAAGAAGCTGGCCGGCTGA

Upstream 100 bases:

>100_bases
GTGGCCCATCCTGGTGGACCGCCCGAGCGGACAATGCAATTGACGCGCAACGTGCCGGCCCGACCCTGGCCGTGCACCGC
CTGAAACAACGGAAACGAAC

Downstream 100 bases:

>100_bases
GCGCTAGCGGCCGAAACCTGCGCTTACCCCGGCGCGGTTTCCTTGCCTGGCCGCACTGCTTATCCTGCCGGCTTGTGCAC
CGGAGCCGTCCCATGCAACG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSRSDWEVVIGLEVHAQLNTASKIFSGASTAFGAEPNVQASAVDIALPGVLPVLNRGAVERAIRFGLAIGATVAPTSVFA
RKNYFYPDLPKGYQISQFELPVVQGGTITIRVGEGENAYEKTVNLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEI
VSEPDMRSSAEAVAYARTLHALVRWIDICDGNMQEGSFRCDANVSVRKKGAEKFGTRREIKNLNSFRFLQQAIDYEVQWQ
IDTIEDGGTIQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPLVISPEWKARVQGEMPELPEAMKARFIEQLGLSA
YDATTLTASKEVATYYQATVDAAGAALAKPCANWVMGDLAARLNKAELDIAVSPVSPAQLAGLVARIADNTISNAIAKKV
FEALWNGEGASADEIIDKQGLKQVTDSGAIEAMIDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASKGKASPAQVNELL
KKKLAG

Sequences:

>Translated_486_residues
MSRSDWEVVIGLEVHAQLNTASKIFSGASTAFGAEPNVQASAVDIALPGVLPVLNRGAVERAIRFGLAIGATVAPTSVFA
RKNYFYPDLPKGYQISQFELPVVQGGTITIRVGEGENAYEKTVNLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEI
VSEPDMRSSAEAVAYARTLHALVRWIDICDGNMQEGSFRCDANVSVRKKGAEKFGTRREIKNLNSFRFLQQAIDYEVQWQ
IDTIEDGGTIQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPLVISPEWKARVQGEMPELPEAMKARFIEQLGLSA
YDATTLTASKEVATYYQATVDAAGAALAKPCANWVMGDLAARLNKAELDIAVSPVSPAQLAGLVARIADNTISNAIAKKV
FEALWNGEGASADEIIDKQGLKQVTDSGAIEAMIDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASKGKASPAQVNELL
KKKLAG
>Mature_485_residues
SRSDWEVVIGLEVHAQLNTASKIFSGASTAFGAEPNVQASAVDIALPGVLPVLNRGAVERAIRFGLAIGATVAPTSVFAR
KNYFYPDLPKGYQISQFELPVVQGGTITIRVGEGENAYEKTVNLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIV
SEPDMRSSAEAVAYARTLHALVRWIDICDGNMQEGSFRCDANVSVRKKGAEKFGTRREIKNLNSFRFLQQAIDYEVQWQI
DTIEDGGTIQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPLVISPEWKARVQGEMPELPEAMKARFIEQLGLSAY
DATTLTASKEVATYYQATVDAAGAALAKPCANWVMGDLAARLNKAELDIAVSPVSPAQLAGLVARIADNTISNAIAKKVF
EALWNGEGASADEIIDKQGLKQVTDSGAIEAMIDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASKGKASPAQVNELLK
KKLAG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=496, Percent_Identity=39.1129032258064, Blast_Score=360, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI115534094, Length=469, Percent_Identity=31.3432835820896, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=31.1663479923518, Blast_Score=223, Evalue=7e-59,
Organism=Drosophila melanogaster, GI45550808, Length=493, Percent_Identity=36.5111561866126, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_AZOSB (A1K1T3)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931675.1
- ProteinModelPortal:   A1K1T3
- SMR:   A1K1T3
- STRING:   A1K1T3
- GeneID:   4606248
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0170
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   A1K1T3
- ProtClustDB:   PRK05477
- BioCyc:   ASP62928:AZO0170-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52678; Mature: 52547

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSDWEVVIGLEVHAQLNTASKIFSGASTAFGAEPNVQASAVDIALPGVLPVLNRGAVE
CCCCCCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCHHHH
RAIRFGLAIGATVAPTSVFARKNYFYPDLPKGYQISQFELPVVQGGTITIRVGEGENAYE
HHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCEECEEECEEEECCEEEEEECCCCCHHH
KTVNLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDMRSSAEAVAYARTLH
HHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
ALVRWIDICDGNMQEGSFRCDANVSVRKKGAEKFGTRREIKNLNSFRFLQQAIDYEVQWQ
HHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCEEEEEE
IDTIEDGGTIQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPLVISPEWKARVQGE
EEEECCCCCEEEEEEEECCCCCHHHHHHCCCHHHCCCCCCCCCCEEEEECCCCHHHCCCC
MPELPEAMKARFIEQLGLSAYDATTLTASKEVATYYQATVDAAGAALAKPCANWVMGDLA
CCCCHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLNKAELDIAVSPVSPAQLAGLVARIADNTISNAIAKKVFEALWNGEGASADEIIDKQG
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LKQVTDSGAIEAMIDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASKGKASPAQVNELL
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KKKLAG
HHHHCC
>Mature Secondary Structure 
SRSDWEVVIGLEVHAQLNTASKIFSGASTAFGAEPNVQASAVDIALPGVLPVLNRGAVE
CCCCCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCHHHH
RAIRFGLAIGATVAPTSVFARKNYFYPDLPKGYQISQFELPVVQGGTITIRVGEGENAYE
HHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCEECEEECEEEECCEEEEEECCCCCHHH
KTVNLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDMRSSAEAVAYARTLH
HHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
ALVRWIDICDGNMQEGSFRCDANVSVRKKGAEKFGTRREIKNLNSFRFLQQAIDYEVQWQ
HHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCEEEEEE
IDTIEDGGTIQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPLVISPEWKARVQGE
EEEECCCCCEEEEEEEECCCCCHHHHHHCCCHHHCCCCCCCCCCEEEEECCCCHHHCCCC
MPELPEAMKARFIEQLGLSAYDATTLTASKEVATYYQATVDAAGAALAKPCANWVMGDLA
CCCCHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLNKAELDIAVSPVSPAQLAGLVARIADNTISNAIAKKVFEALWNGEGASADEIIDKQG
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LKQVTDSGAIEAMIDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASKGKASPAQVNELL
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KKKLAG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA