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The map label for this gene is yafJ [C]

Identifier: 119896460

GI number: 119896460

Start: 184801

End: 185625

Strand: Reverse

Name: yafJ [C]

Synonym: azo0168

Alternate gene names: 119896460

Gene position: 185625-184801 (Counterclockwise)

Preceding gene: 119896461

Following gene: 119896459

Centisome position: 4.24

GC content: 67.64

Gene sequence:

>825_bases
ATGTGCCAGCTGCTGGGCATGAACTGCAATGTGCCCACCGACATCTGCTTTTCCTTCACCGGCTTTCGCGCCCGCGGCGG
CCTGACCGACCACCACCGTGACGGCTGGGGCATCGCGTTCTTCGAAGGCAAGGGCGTGCGCCTGTTCCTCGATCCCTGCG
CCAGCGCCGACTCCGCAGTCGCCGAGCTGGTGCGGCAATACCCGATCCGCTCGCTCAACGTCATCGCTCACATCCGCAAG
GCGACCCAGGGCGAGATCCGGCTTGAAAACACCCACCCGTTCCAGCGCGAGCTGTGGGGGCGCTACTGGATATTCGCCCA
TAACGGCAACCTGCTGAACTACGCGCCGGCCTTGTCCGGCCGCTTTCAGCCGGTCGGCGGCACCGACTCCGAAACCGCGT
TCTGCCACATCCTCGACGAACTCGCCCGCCGTTTCGGCGACCGCCCGCCGCCGGCGCCCGCGCTGCACGCCTGCCTGGCC
GAACTGGCAACCGCGATCGGCCGCCACGGCGAATTCAACTTCCTGCTGTCGGACGGCGAGCGCCTGTTTGCCCATTGCTC
CTCGCGCCTCGCCTACGTGCTGCGACAAGCGCCGTTTGCGCTGGCCCATCTGGCGGACGAAGACCTGGCGGTGGACTTCA
GCGAGGTCACCACGCCCAACGACCGGGTGGCGGTGATTGCGACCATTCCGCTGACCGACAACGAGACCTGGCACCGGATG
CCGTCCGGCACCCTGATGTCGTTCCGCCACGGCGTGCCCGACGAACTCGGGATGCTGCCCACGGTGGCCGCACCGCCCTG
CGCCACCCCCATCCGAAGTGCGTAA

Upstream 100 bases:

>100_bases
TCTCGACGCGATGATGAAGGGCATGCAGCGCCGCTGAGGGACACGGCGGCAGCCAGCCCGGCGCCTATAATCGAACTCCC
GTTCGCCGCCGGAGCCCGCC

Downstream 100 bases:

>100_bases
ATTTTCCTTATCCACGCCGATGCCGGCGCGTTAAAGTTGGCATCCCCTCCGGCCAGGTTTGCCCGCCATGCAAGTGGATT
GCCCGCTGTGCCAGATCGAC

Product: glutamine amidotransferases class-II-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MCQLLGMNCNVPTDICFSFTGFRARGGLTDHHRDGWGIAFFEGKGVRLFLDPCASADSAVAELVRQYPIRSLNVIAHIRK
ATQGEIRLENTHPFQRELWGRYWIFAHNGNLLNYAPALSGRFQPVGGTDSETAFCHILDELARRFGDRPPPAPALHACLA
ELATAIGRHGEFNFLLSDGERLFAHCSSRLAYVLRQAPFALAHLADEDLAVDFSEVTTPNDRVAVIATIPLTDNETWHRM
PSGTLMSFRHGVPDELGMLPTVAAPPCATPIRSA

Sequences:

>Translated_274_residues
MCQLLGMNCNVPTDICFSFTGFRARGGLTDHHRDGWGIAFFEGKGVRLFLDPCASADSAVAELVRQYPIRSLNVIAHIRK
ATQGEIRLENTHPFQRELWGRYWIFAHNGNLLNYAPALSGRFQPVGGTDSETAFCHILDELARRFGDRPPPAPALHACLA
ELATAIGRHGEFNFLLSDGERLFAHCSSRLAYVLRQAPFALAHLADEDLAVDFSEVTTPNDRVAVIATIPLTDNETWHRM
PSGTLMSFRHGVPDELGMLPTVAAPPCATPIRSA
>Mature_274_residues
MCQLLGMNCNVPTDICFSFTGFRARGGLTDHHRDGWGIAFFEGKGVRLFLDPCASADSAVAELVRQYPIRSLNVIAHIRK
ATQGEIRLENTHPFQRELWGRYWIFAHNGNLLNYAPALSGRFQPVGGTDSETAFCHILDELARRFGDRPPPAPALHACLA
ELATAIGRHGEFNFLLSDGERLFAHCSSRLAYVLRQAPFALAHLADEDLAVDFSEVTTPNDRVAVIATIPLTDNETWHRM
PSGTLMSFRHGVPDELGMLPTVAAPPCATPIRSA

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=248, Percent_Identity=54.8387096774194, Blast_Score=287, Evalue=6e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 30177; Mature: 30177

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCNVPTDICFSFTGFRARGGLTDHHRDGWGIAFFEGKGVRLFLDPCASADSAV
CCEEECCCCCCCHHHHHEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHH
AELVRQYPIRSLNVIAHIRKATQGEIRLENTHPFQRELWGRYWIFAHNGNLLNYAPALSG
HHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCEEEEEEECCCEEEECCCCCC
RFQPVGGTDSETAFCHILDELARRFGDRPPPAPALHACLAELATAIGRHGEFNFLLSDGE
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHH
RLFAHCSSRLAYVLRQAPFALAHLADEDLAVDFSEVTTPNDRVAVIATIPLTDNETWHRM
HHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHCCCCCCCEEEEEEEECCCCCHHHCC
PSGTLMSFRHGVPDELGMLPTVAAPPCATPIRSA
CCCCHHHHHCCCCHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCQLLGMNCNVPTDICFSFTGFRARGGLTDHHRDGWGIAFFEGKGVRLFLDPCASADSAV
CCEEECCCCCCCHHHHHEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHH
AELVRQYPIRSLNVIAHIRKATQGEIRLENTHPFQRELWGRYWIFAHNGNLLNYAPALSG
HHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCEEEEEEECCCEEEECCCCCC
RFQPVGGTDSETAFCHILDELARRFGDRPPPAPALHACLAELATAIGRHGEFNFLLSDGE
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHH
RLFAHCSSRLAYVLRQAPFALAHLADEDLAVDFSEVTTPNDRVAVIATIPLTDNETWHRM
HHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHCCCCCCCEEEEEEEECCCCCHHHCC
PSGTLMSFRHGVPDELGMLPTVAAPPCATPIRSA
CCCCHHHHHCCCCHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]