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The map label for this gene is topB [H]

Identifier: 119896387

GI number: 119896387

Start: 112981

End: 115554

Strand: Reverse

Name: topB [H]

Synonym: azo0095

Alternate gene names: 119896387

Gene position: 115554-112981 (Counterclockwise)

Preceding gene: 119896388

Following gene: 119896385

Centisome position: 2.64

GC content: 66.24

Gene sequence:

>2574_bases
ATGAGCAAGCAACTGATCATCGCGGAGAAGCCCTCCGTCGCGCAGGACATCGCCCGTGCGCTGGGCGGCTTCACCAAGGA
GAAGGACTACTTCGAGTCCGACGATTACGTGTTGTCGTCGGCCGTCGGCCACCTTCTCGAGCTTGCCGTCCCCGAGGAAT
TCGAGGTCAAGCGCGGCAAATGGACCTTCGCCCACCTGCCGGTGATTCCGCCGCATTTCGCGCTGAAGCCGATCGAAAAG
ACGGACGACCGCCTGAAACTGCTGACCCGGCTGATCAAGCGCAAGGACGTCACCGGCCTGATCAACGCCTGTGACGCGGG
CCGCGAAGGCGAGCTGATCTTCAACTTCATCGCCCAGCACGCGGGCACCAACAAGCCCATGCAACGGCTGTGGCTGCAGT
CGATGACGCCGGCGGCGATCCGCGACGGCTTTGCCCACCTGCGCGCCGCGCGCGACGTCGAAGGCTTGCGCAATGCCGCG
ATCTGCCGCGCCGAGTCCGACTGGCTGATCGGCATCAACGGCACCCGCGCGATGACCGCGTTCAACTCCAAGACCGGCGG
CTTCCACCTCACCACCGTCGGTCGGGTGCAGACACCCACGCTCGCCATCGTGGTCGAGCGCGAGGACAAGATCCGCAAAT
TCAAGCCGCGCGACTACTGGGAGCTGGAAGCCACCTTCGGCTGCGCCGCCGGCGAGTACACCGGGCGCTGGCTGGACGAG
AACTTCAGGAAGCCCGAAGGCGACGAACACGCCAACGCCCACCGGCTGTGGGACAAGGCCAGGGCGGAAGCCATCCGCGC
GAAGTGCGAGGGCAAACCGGGCGTCGTCACCGAGGAAGCCAAACCCTCCACCCAGCTCTCGCCGCTGCTGTTCGACCTCA
CCAGCCTGCAGCGCGAAGCCAACGGCCGCTTCGGCTTCTCTGCCCGCGTCACGCTGCAGCTGGCGCAGGCGCTGTACGAA
AAGCACAAGGTGCTGACCTATCCGCGTACCGACGCGCGCGCGCTGCCCGAGGACTACCTCGGCACCGTGAAGGACGTGAT
GGCCGGCCTGCCCGACACCTACGCGCCCTTCGCCAACGAGATCGCCAAGCAGGGCTGGGTCAGGCCCAACAAGCGCATCT
TCAACAACGCCAAGATCTCCGACCACTTCGCGATCATCCCCACCGGGACGCTGCCGAAGAGCCTGTCGGAAGCCGAACAC
AAGATCTACGACCTGGTGACCCGCCGCTTCCTGTCGGTGTTCTACCCGGCCGCCGAGTACCAGATCACCACCCGCATCAC
CCGCGTCGAGGGCGAAGCCTTCAAGACCGAGGGCAAGGTGCTGGTCAACGCCGGCTGGCTGGCGGTGTATGGCAAGGAAG
CGGCGAACGAGGACGGTGAGGGCAAGGACGCCGGCAAGGACAGCGGGCGCCAGCTGGTGGCGGTCAAGCCGGGCGAGACG
GTGTCCACCGACGACATCCTGGTCAAGGCGCTGCAGACCAAGCCGCCGGCCCGCTTCAACGAAGCCACGCTGCTGTCGGC
AATGGAAGGCGCCGGCAAGATGGTGGACGACGAGGAACTGCGCGCGGCAATGGCGGAACGCGGCCTCGGCACCCCGGCCA
CGCGGGCGCAGATCATCGAAGGTCTGATCGCCGAGCAGTACATCCACCGCGAAGGCCGCGAGCTGATCCCCAGCGCCAAG
GCCTTCTCGCTGATCACGCTGCTGACCGGCCTCGGCGTCAATGCGCTGACCTCGCCCGAACTCACCGGCGGCTGGGAATA
CAAGCTGTCGCGCATGGAACGCGGCGAACTCTCGCGCGAAGCCTTCATGCACGAGATCGCCGAGATGGCGCGCGAGGTGG
TGGAACGCGCCAAACGCTACGAATCGGACACCGTGCCGGGCGACTTCGTCACGCTGAAGACGCCGTGCCCGAAGTGCGGC
GGCACCGTGAAGGAGAACTACAAGAAGTTCGCCTGCCAGGCGTGCGACTGGAGCACCTGGAAGATCGTCGCCGGGCGCCA
GTTCGAGATCGACGAGATCGAGACCCTGCTGCGCGACGGCAAGGTCGGCCCGCTGCTCGGCTTCCGCAACAAGATGGGGC
GGCTGTTCAACGCCGAGATCAAGCTCAACGACGACAAGCAGCCGGAGTTCGACTTCGGCCAACCCAAGGAAGGCGAGGAA
GGCGGCGAAGCGGTCGATTTCTCCGGCCAGCAGTCCATCGGCGCCTGCCCCAAGTGCGGCGCCAACGTGTTCGAGCACGG
CCTCGCCTATGTCTGCGAGAAGTCCGTCGGCCCCGGCAAGAACTGCGACTTCCGCTCCGGCAAGATCATCCTGCAGCAAC
CGATCGAACGCGAGCAGATGGCCAAGCTGCTGACCGACGGCAAGACCGACCTGCTGAAGGGTTTCGTGTCGGCACGTACG
CGGCGCAAGTTCTCCGCCTTCCTGGTGCGCGGCAAAGATGGCAAGGTCGGCTTCGAGTTCGAGGCCAAGACCCCCAAGGC
GGGCGCCAAGAGCACGCCCGCCAAGGCCGCGGCCAACGAGGAAACCGGCAAGCCCGCGCCCCGCAAGCGCGCGGGTGGCA
AGACCGCCGGCTGA

Upstream 100 bases:

>100_bases
ATCTGGCTTGCACGGCGGGCGCGCGGCTCTTTATCATCCGCCGCTTCACAACCCTAACGGACGGACAGCCGGAAACGGCC
CTCCGCACCTGCCCCCCGGC

Downstream 100 bases:

>100_bases
GTTTTCGCCGCGATGCAGCCAGCAAAACGGCCCGTGAGGGCCGTTTTGCGTGTTGGAGTATCCGACCTCAGGCCGCTTCC
ACCGCGGGCACCCGTCCGAT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 857; Mature: 856

Protein sequence:

>857_residues
MSKQLIIAEKPSVAQDIARALGGFTKEKDYFESDDYVLSSAVGHLLELAVPEEFEVKRGKWTFAHLPVIPPHFALKPIEK
TDDRLKLLTRLIKRKDVTGLINACDAGREGELIFNFIAQHAGTNKPMQRLWLQSMTPAAIRDGFAHLRAARDVEGLRNAA
ICRAESDWLIGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAIVVEREDKIRKFKPRDYWELEATFGCAAGEYTGRWLDE
NFRKPEGDEHANAHRLWDKARAEAIRAKCEGKPGVVTEEAKPSTQLSPLLFDLTSLQREANGRFGFSARVTLQLAQALYE
KHKVLTYPRTDARALPEDYLGTVKDVMAGLPDTYAPFANEIAKQGWVRPNKRIFNNAKISDHFAIIPTGTLPKSLSEAEH
KIYDLVTRRFLSVFYPAAEYQITTRITRVEGEAFKTEGKVLVNAGWLAVYGKEAANEDGEGKDAGKDSGRQLVAVKPGET
VSTDDILVKALQTKPPARFNEATLLSAMEGAGKMVDDEELRAAMAERGLGTPATRAQIIEGLIAEQYIHREGRELIPSAK
AFSLITLLTGLGVNALTSPELTGGWEYKLSRMERGELSREAFMHEIAEMAREVVERAKRYESDTVPGDFVTLKTPCPKCG
GTVKENYKKFACQACDWSTWKIVAGRQFEIDEIETLLRDGKVGPLLGFRNKMGRLFNAEIKLNDDKQPEFDFGQPKEGEE
GGEAVDFSGQQSIGACPKCGANVFEHGLAYVCEKSVGPGKNCDFRSGKIILQQPIEREQMAKLLTDGKTDLLKGFVSART
RRKFSAFLVRGKDGKVGFEFEAKTPKAGAKSTPAKAAANEETGKPAPRKRAGGKTAG

Sequences:

>Translated_857_residues
MSKQLIIAEKPSVAQDIARALGGFTKEKDYFESDDYVLSSAVGHLLELAVPEEFEVKRGKWTFAHLPVIPPHFALKPIEK
TDDRLKLLTRLIKRKDVTGLINACDAGREGELIFNFIAQHAGTNKPMQRLWLQSMTPAAIRDGFAHLRAARDVEGLRNAA
ICRAESDWLIGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAIVVEREDKIRKFKPRDYWELEATFGCAAGEYTGRWLDE
NFRKPEGDEHANAHRLWDKARAEAIRAKCEGKPGVVTEEAKPSTQLSPLLFDLTSLQREANGRFGFSARVTLQLAQALYE
KHKVLTYPRTDARALPEDYLGTVKDVMAGLPDTYAPFANEIAKQGWVRPNKRIFNNAKISDHFAIIPTGTLPKSLSEAEH
KIYDLVTRRFLSVFYPAAEYQITTRITRVEGEAFKTEGKVLVNAGWLAVYGKEAANEDGEGKDAGKDSGRQLVAVKPGET
VSTDDILVKALQTKPPARFNEATLLSAMEGAGKMVDDEELRAAMAERGLGTPATRAQIIEGLIAEQYIHREGRELIPSAK
AFSLITLLTGLGVNALTSPELTGGWEYKLSRMERGELSREAFMHEIAEMAREVVERAKRYESDTVPGDFVTLKTPCPKCG
GTVKENYKKFACQACDWSTWKIVAGRQFEIDEIETLLRDGKVGPLLGFRNKMGRLFNAEIKLNDDKQPEFDFGQPKEGEE
GGEAVDFSGQQSIGACPKCGANVFEHGLAYVCEKSVGPGKNCDFRSGKIILQQPIEREQMAKLLTDGKTDLLKGFVSART
RRKFSAFLVRGKDGKVGFEFEAKTPKAGAKSTPAKAAANEETGKPAPRKRAGGKTAG
>Mature_856_residues
SKQLIIAEKPSVAQDIARALGGFTKEKDYFESDDYVLSSAVGHLLELAVPEEFEVKRGKWTFAHLPVIPPHFALKPIEKT
DDRLKLLTRLIKRKDVTGLINACDAGREGELIFNFIAQHAGTNKPMQRLWLQSMTPAAIRDGFAHLRAARDVEGLRNAAI
CRAESDWLIGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAIVVEREDKIRKFKPRDYWELEATFGCAAGEYTGRWLDEN
FRKPEGDEHANAHRLWDKARAEAIRAKCEGKPGVVTEEAKPSTQLSPLLFDLTSLQREANGRFGFSARVTLQLAQALYEK
HKVLTYPRTDARALPEDYLGTVKDVMAGLPDTYAPFANEIAKQGWVRPNKRIFNNAKISDHFAIIPTGTLPKSLSEAEHK
IYDLVTRRFLSVFYPAAEYQITTRITRVEGEAFKTEGKVLVNAGWLAVYGKEAANEDGEGKDAGKDSGRQLVAVKPGETV
STDDILVKALQTKPPARFNEATLLSAMEGAGKMVDDEELRAAMAERGLGTPATRAQIIEGLIAEQYIHREGRELIPSAKA
FSLITLLTGLGVNALTSPELTGGWEYKLSRMERGELSREAFMHEIAEMAREVVERAKRYESDTVPGDFVTLKTPCPKCGG
TVKENYKKFACQACDWSTWKIVAGRQFEIDEIETLLRDGKVGPLLGFRNKMGRLFNAEIKLNDDKQPEFDFGQPKEGEEG
GEAVDFSGQQSIGACPKCGANVFEHGLAYVCEKSVGPGKNCDFRSGKIILQQPIEREQMAKLLTDGKTDLLKGFVSARTR
RKFSAFLVRGKDGKVGFEFEAKTPKAGAKSTPAKAAANEETGKPAPRKRAGGKTAG

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=832, Percent_Identity=25.1201923076923, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI10835218, Length=661, Percent_Identity=23.9031770045386, Blast_Score=117, Evalue=6e-26,
Organism=Escherichia coli, GI1788061, Length=628, Percent_Identity=31.8471337579618, Blast_Score=309, Evalue=4e-85,
Organism=Escherichia coli, GI1787529, Length=678, Percent_Identity=25.811209439528, Blast_Score=144, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17555378, Length=665, Percent_Identity=25.4135338345865, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=605, Percent_Identity=25.1239669421488, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640096, Length=603, Percent_Identity=24.5439469320066, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585251, Length=638, Percent_Identity=24.294670846395, Blast_Score=105, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 94649; Mature: 94518

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQLIIAEKPSVAQDIARALGGFTKEKDYFESDDYVLSSAVGHLLELAVPEEFEVKRGK
CCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
WTFAHLPVIPPHFALKPIEKTDDRLKLLTRLIKRKDVTGLINACDAGREGELIFNFIAQH
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHH
AGTNKPMQRLWLQSMTPAAIRDGFAHLRAARDVEGLRNAAICRAESDWLIGINGTRAMTA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCEEEEE
FNSKTGGFHLTTVGRVQTPTLAIVVEREDKIRKFKPRDYWELEATFGCAAGEYTGRWLDE
ECCCCCCEEEEEECCCCCCEEEEEEECCHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHH
NFRKPEGDEHANAHRLWDKARAEAIRAKCEGKPGVVTEEAKPSTQLSPLLFDLTSLQREA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHH
NGRFGFSARVTLQLAQALYEKHKVLTYPRTDARALPEDYLGTVKDVMAGLPDTYAPFANE
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCHHHHHH
IAKQGWVRPNKRIFNNAKISDHFAIIPTGTLPKSLSEAEHKIYDLVTRRFLSVFYPAAEY
HHHHCCCCCCHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
QITTRITRVEGEAFKTEGKVLVNAGWLAVYGKEAANEDGEGKDAGKDSGRQLVAVKPGET
EEEEEEEEECCCCEECCCCEEEECCEEEEECCHHCCCCCCCCCCCCCCCCEEEEECCCCC
VSTDDILVKALQTKPPARFNEATLLSAMEGAGKMVDDEELRAAMAERGLGTPATRAQIIE
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
GLIAEQYIHREGRELIPSAKAFSLITLLTGLGVNALTSPELTGGWEYKLSRMERGELSRE
HHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AFMHEIAEMAREVVERAKRYESDTVPGDFVTLKTPCPKCGGTVKENYKKFACQACDWSTW
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCE
KIVAGRQFEIDEIETLLRDGKVGPLLGFRNKMGRLFNAEIKLNDDKQPEFDFGQPKEGEE
EEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCC
GGEAVDFSGQQSIGACPKCGANVFEHGLAYVCEKSVGPGKNCDFRSGKIILQQPIEREQM
CCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCHHHH
AKLLTDGKTDLLKGFVSARTRRKFSAFLVRGKDGKVGFEFEAKTPKAGAKSTPAKAAANE
HHHHHCCHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCHHCCCC
ETGKPAPRKRAGGKTAG
CCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SKQLIIAEKPSVAQDIARALGGFTKEKDYFESDDYVLSSAVGHLLELAVPEEFEVKRGK
CCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
WTFAHLPVIPPHFALKPIEKTDDRLKLLTRLIKRKDVTGLINACDAGREGELIFNFIAQH
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHH
AGTNKPMQRLWLQSMTPAAIRDGFAHLRAARDVEGLRNAAICRAESDWLIGINGTRAMTA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCEEEEE
FNSKTGGFHLTTVGRVQTPTLAIVVEREDKIRKFKPRDYWELEATFGCAAGEYTGRWLDE
ECCCCCCEEEEEECCCCCCEEEEEEECCHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHH
NFRKPEGDEHANAHRLWDKARAEAIRAKCEGKPGVVTEEAKPSTQLSPLLFDLTSLQREA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHH
NGRFGFSARVTLQLAQALYEKHKVLTYPRTDARALPEDYLGTVKDVMAGLPDTYAPFANE
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCHHHHHH
IAKQGWVRPNKRIFNNAKISDHFAIIPTGTLPKSLSEAEHKIYDLVTRRFLSVFYPAAEY
HHHHCCCCCCHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
QITTRITRVEGEAFKTEGKVLVNAGWLAVYGKEAANEDGEGKDAGKDSGRQLVAVKPGET
EEEEEEEEECCCCEECCCCEEEECCEEEEECCHHCCCCCCCCCCCCCCCCEEEEECCCCC
VSTDDILVKALQTKPPARFNEATLLSAMEGAGKMVDDEELRAAMAERGLGTPATRAQIIE
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
GLIAEQYIHREGRELIPSAKAFSLITLLTGLGVNALTSPELTGGWEYKLSRMERGELSRE
HHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AFMHEIAEMAREVVERAKRYESDTVPGDFVTLKTPCPKCGGTVKENYKKFACQACDWSTW
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCE
KIVAGRQFEIDEIETLLRDGKVGPLLGFRNKMGRLFNAEIKLNDDKQPEFDFGQPKEGEE
EEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCC
GGEAVDFSGQQSIGACPKCGANVFEHGLAYVCEKSVGPGKNCDFRSGKIILQQPIEREQM
CCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCHHHH
AKLLTDGKTDLLKGFVSARTRRKFSAFLVRGKDGKVGFEFEAKTPKAGAKSTPAKAAANE
HHHHHCCHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCHHCCCC
ETGKPAPRKRAGGKTAG
CCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA