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The map label for this gene is rsuA [H]

Identifier: 119896376

GI number: 119896376

Start: 96022

End: 96768

Strand: Direct

Name: rsuA [H]

Synonym: azo0084

Alternate gene names: 119896376

Gene position: 96022-96768 (Clockwise)

Preceding gene: 119896373

Following gene: 119896377

Centisome position: 2.19

GC content: 67.87

Gene sequence:

>747_bases
ATGCAGGCCATGCAAATCGAACGCCTCCTCCATTCCCAGGGCTTCGGCAGCCGCAAGGACTGCCGCGCCCTCCTCCGTGC
CGGCTACGTCAGCGTCGCCGGCGTGCCCTGCGACGACCCCCGCGCCGAATTCGACCCCGGCAGCAAGGACGGCAGTCCGG
GGCTCGAATTCACCGTCGATGACGAGATCTGGCACTTCCGCGCGCAGGCCTATCTGATGCTGCACAAGCCCGCGGGCTAC
GAGTGTTCGCACAAGCCCACCTTCCATCCCTCGGTGTTCACGCTGTTGCCGCCGCAGTTGCTCAACCGTGGCGTGCAGTG
CATCGGCCGGCTGGACCAGGACACCACCGGACTGTTGCTGCTGTCGGACGACGGGCAGTTCATCCACCAGTGGAGTTCGG
GCAAGAAGCGCACGCCCAAGGTGTACGAGATCGGCCTCAAGCACGCAGCCGACGACGCCCTGGTGGCGGCGCTGCTGGCC
GGTGTCGAACTCCACGACGAACCGACGCCGATCGCGGCCGCTGCGTGCGAGATCACCGCCAGCCACGCGCTGCGGCTGAC
GATCACCGAAGGCAAGTATCACCAGGTCAAGCGCATGGTGGCCGCGGCGGGCAACCGGGTGGAAACCCTGCATCGCAGCC
GCGTCGGCGGCCTGACGCTGGATCCCGCCTTGAAGCCCGGCGAATGGCGCTGGCTCGAAGCGGCCGATATCGCTGCGTTG
GCCGCGGGTGCGGCCGACGGCGATTGA

Upstream 100 bases:

>100_bases
CGACCAGGTGTTCGAACTTCACGGACTTCTCCCTCGCGGGTCGGCTCCGCAGTTTACCGCGAGCCGGGGCGCACTCGCAG
GTCCGGGCATCCGGGCTACC

Downstream 100 bases:

>100_bases
AGCGCCAGCCAGCAGGGGTAGGTTTTATCTTGTTTTTTTAATTATTAAAGAAATAAAGAAGTTAACAGGGGACGAAAATC
TGTGGATAACTGGCTGAAAC

Product: pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MQAMQIERLLHSQGFGSRKDCRALLRAGYVSVAGVPCDDPRAEFDPGSKDGSPGLEFTVDDEIWHFRAQAYLMLHKPAGY
ECSHKPTFHPSVFTLLPPQLLNRGVQCIGRLDQDTTGLLLLSDDGQFIHQWSSGKKRTPKVYEIGLKHAADDALVAALLA
GVELHDEPTPIAAAACEITASHALRLTITEGKYHQVKRMVAAAGNRVETLHRSRVGGLTLDPALKPGEWRWLEAADIAAL
AAGAADGD

Sequences:

>Translated_248_residues
MQAMQIERLLHSQGFGSRKDCRALLRAGYVSVAGVPCDDPRAEFDPGSKDGSPGLEFTVDDEIWHFRAQAYLMLHKPAGY
ECSHKPTFHPSVFTLLPPQLLNRGVQCIGRLDQDTTGLLLLSDDGQFIHQWSSGKKRTPKVYEIGLKHAADDALVAALLA
GVELHDEPTPIAAAACEITASHALRLTITEGKYHQVKRMVAAAGNRVETLHRSRVGGLTLDPALKPGEWRWLEAADIAAL
AAGAADGD
>Mature_248_residues
MQAMQIERLLHSQGFGSRKDCRALLRAGYVSVAGVPCDDPRAEFDPGSKDGSPGLEFTVDDEIWHFRAQAYLMLHKPAGY
ECSHKPTFHPSVFTLLPPQLLNRGVQCIGRLDQDTTGLLLLSDDGQFIHQWSSGKKRTPKVYEIGLKHAADDALVAALLA
GVELHDEPTPIAAAACEITASHALRLTITEGKYHQVKRMVAAAGNRVETLHRSRVGGLTLDPALKPGEWRWLEAADIAAL
AAGAADGD

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=171, Percent_Identity=43.859649122807, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=29.5546558704453, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI87081838, Length=145, Percent_Identity=32.4137931034483, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26907; Mature: 26907

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAMQIERLLHSQGFGSRKDCRALLRAGYVSVAGVPCDDPRAEFDPGSKDGSPGLEFTVD
CCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEC
DEIWHFRAQAYLMLHKPAGYECSHKPTFHPSVFTLLPPQLLNRGVQCIGRLDQDTTGLLL
CHHHHEEEEEEEEEECCCCCCCCCCCCCCCCHHEECCHHHHHHHHHHHHCCCCCCCEEEE
LSDDGQFIHQWSSGKKRTPKVYEIGLKHAADDALVAALLAGVELHDEPTPIAAAACEITA
ECCCCCEEHHHCCCCCCCCHHEEEHHHHCCHHHHHHHHHHCCEECCCCCCHHHHHHHEEC
SHALRLTITEGKYHQVKRMVAAAGNRVETLHRSRVGGLTLDPALKPGEWRWLEAADIAAL
CCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCCCCCCEEEECHHHHHHH
AAGAADGD
HCCCCCCC
>Mature Secondary Structure
MQAMQIERLLHSQGFGSRKDCRALLRAGYVSVAGVPCDDPRAEFDPGSKDGSPGLEFTVD
CCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEC
DEIWHFRAQAYLMLHKPAGYECSHKPTFHPSVFTLLPPQLLNRGVQCIGRLDQDTTGLLL
CHHHHEEEEEEEEEECCCCCCCCCCCCCCCCHHEECCHHHHHHHHHHHHCCCCCCCEEEE
LSDDGQFIHQWSSGKKRTPKVYEIGLKHAADDALVAALLAGVELHDEPTPIAAAACEITA
ECCCCCEEHHHCCCCCCCCHHEEEHHHHCCHHHHHHHHHHCCEECCCCCCHHHHHHHEEC
SHALRLTITEGKYHQVKRMVAAAGNRVETLHRSRVGGLTLDPALKPGEWRWLEAADIAAL
CCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCCCCCCEEEECHHHHHHH
AAGAADGD
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]