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The map label for this gene is dhaAF [H]

Identifier: 119871453

GI number: 119871453

Start: 5661890

End: 5662654

Strand: Direct

Name: dhaAF [H]

Synonym: Mkms_5429

Alternate gene names: 119871453

Gene position: 5661890-5662654 (Clockwise)

Preceding gene: 119871452

Following gene: 119871454

Centisome position: 98.69

GC content: 67.19

Gene sequence:

>765_bases
ATGGTGCCGGTGGAAGACACCGCCCTGGCCACGACCGACAGCGGCGGGACTGGTGTCCCCCTGGTCTACCTGAACGGTTC
CTACGGCAGCCAGCGACACTGGCGGCGCGTCATCGACGAGCTGGGACCGGGATGGCGGCACATCACCTACGACGAACGCG
CGCGCGGCAAGTCGACGAGATCAGCGGACTACTCCTTCGAGGCCACCGTCCGGGATCTCGACGCCGTCCTGCGAGCCAGA
GAAGTGGACGAGCCGATCCTGGTGGGCTGGTCCTACGGCGCGTTGATCGCGGTGCAGTGGGCCGCGCGACACCCGAGCCG
CGTCGCCGGCGTGGTCGGCGTCGACGGCCCCTACCCGGATGGCTGGACCGATGAGGCCGCCCATGAATACATCAGGCGGT
CCTTCCGTAGGTATCGGTGGGTGTTGCCGATGGCGCGCCTCATCGGCAACGCCGCGAGGATGAGTGCGGACCAACATGCC
GAGATCAACATCGAGGCGCACAAGATCCACGCCGCGCTCGAACCGATCCTGGAAAGCGTCACCGTCCCGGTCCGGTACGT
CGTCGCCTCCGGCGAGTACCTCGGAAGCAAAGACAACGCACAGGAGCGCATGCGCACTACCCTGAGACCGGTGCTCGCCC
GCAACCCGAACCTCGCTGTCAGCGCGAAGGTCGACAGCAACCACGGCACGATCGTGCGCAAGGATTTCCGCGCCATCGCC
CGGGCGGTACGCGAAGTCGCCGCCTTCGGCCGCCAAAGTCGTTGA

Upstream 100 bases:

>100_bases
GGCCAGATGCAGACCAGATAGCCGTTGACTCTGCCCTTGGGTCAGGGTCGACGCTGGTCGCATGACGAACCCACACGCGA
CCACCTCGGCCTGGCGTGGC

Downstream 100 bases:

>100_bases
CCGCCAACACTGCTGGCGTCACCATCGGCCGAGCAGCGGCCTTCGCCGGCGTCACGATCAAGACGGTCCGCCACTACCAC
CGGATCGGCCTGCTCGACGA

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MVPVEDTALATTDSGGTGVPLVYLNGSYGSQRHWRRVIDELGPGWRHITYDERARGKSTRSADYSFEATVRDLDAVLRAR
EVDEPILVGWSYGALIAVQWAARHPSRVAGVVGVDGPYPDGWTDEAAHEYIRRSFRRYRWVLPMARLIGNAARMSADQHA
EINIEAHKIHAALEPILESVTVPVRYVVASGEYLGSKDNAQERMRTTLRPVLARNPNLAVSAKVDSNHGTIVRKDFRAIA
RAVREVAAFGRQSR

Sequences:

>Translated_254_residues
MVPVEDTALATTDSGGTGVPLVYLNGSYGSQRHWRRVIDELGPGWRHITYDERARGKSTRSADYSFEATVRDLDAVLRAR
EVDEPILVGWSYGALIAVQWAARHPSRVAGVVGVDGPYPDGWTDEAAHEYIRRSFRRYRWVLPMARLIGNAARMSADQHA
EINIEAHKIHAALEPILESVTVPVRYVVASGEYLGSKDNAQERMRTTLRPVLARNPNLAVSAKVDSNHGTIVRKDFRAIA
RAVREVAAFGRQSR
>Mature_254_residues
MVPVEDTALATTDSGGTGVPLVYLNGSYGSQRHWRRVIDELGPGWRHITYDERARGKSTRSADYSFEATVRDLDAVLRAR
EVDEPILVGWSYGALIAVQWAARHPSRVAGVVGVDGPYPDGWTDEAAHEYIRRSFRRYRWVLPMARLIGNAARMSADQHA
EINIEAHKIHAALEPILESVTVPVRYVVASGEYLGSKDNAQERMRTTLRPVLARNPNLAVSAKVDSNHGTIVRKDFRAIA
RAVREVAAFGRQSR

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 28283; Mature: 28283

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPVEDTALATTDSGGTGVPLVYLNGSYGSQRHWRRVIDELGPGWRHITYDERARGKSTR
CCCCCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCHHHCCCCCC
SADYSFEATVRDLDAVLRAREVDEPILVGWSYGALIAVQWAARHPSRVAGVVGVDGPYPD
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHCCCCCEEEEEECCCCCCC
GWTDEAAHEYIRRSFRRYRWVLPMARLIGNAARMSADQHAEINIEAHKIHAALEPILESV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
TVPVRYVVASGEYLGSKDNAQERMRTTLRPVLARNPNLAVSAKVDSNHGTIVRKDFRAIA
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEHHHHHHHH
RAVREVAAFGRQSR
HHHHHHHHHCCCCC
>Mature Secondary Structure
MVPVEDTALATTDSGGTGVPLVYLNGSYGSQRHWRRVIDELGPGWRHITYDERARGKSTR
CCCCCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCHHHCCCCCC
SADYSFEATVRDLDAVLRAREVDEPILVGWSYGALIAVQWAARHPSRVAGVVGVDGPYPD
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHCCCCCEEEEEECCCCCCC
GWTDEAAHEYIRRSFRRYRWVLPMARLIGNAARMSADQHAEINIEAHKIHAALEPILESV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
TVPVRYVVASGEYLGSKDNAQERMRTTLRPVLARNPNLAVSAKVDSNHGTIVRKDFRAIA
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEHHHHHHHH
RAVREVAAFGRQSR
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]