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The map label for this gene is ubiE [H]

Identifier: 119871194

GI number: 119871194

Start: 5414784

End: 5415500

Strand: Direct

Name: ubiE [H]

Synonym: Mkms_5167

Alternate gene names: 119871194

Gene position: 5414784-5415500 (Clockwise)

Preceding gene: 119871193

Following gene: 119871195

Centisome position: 94.38

GC content: 70.57

Gene sequence:

>717_bases
ATGAGTGACGCGCTGCCCCACGCCCAGGTGCCCGCCGCGTTCGACGTCGGCGCGGCCGCCTACGACAAGCTCGTCGGCGC
CAATCCCGGTTACCACGACCATCTCCGGCTGTCCGCGCAGCGGATGGGCCTGCCCGACGGCGGACGCGGTATGCGCCTGC
TCGATGCGGGCTGCGGCACCGGCGCATCGACCGCCGCGCTGCTCGCCGCCGCCCCGCACGCCGAGATCGTCGCCGTCGAC
GCCTCGGCCGGGATGCTCGCCGAGGCGCGGTCGAAGGCGTGGCCCGGCTCGGTGCGCTTCGTCCACAGCCGCATCGAGGA
CATCGCCGACGCCGACGTGACCGGACCGTTCGACGGGATCTTCGCCGCCTATCTGCTGCGCAACCTCGCCGACCCGGACA
CCCAACTGCGGACGTTCCGCGACATGCTCAACCCCGGCGCACCGCTGACCGTGCACGAATACTCGGTGCGCGACTCCGCC
GTGGCGACCGCGGTGTGGAATGCGGTGTGCTGGGCGATCATCATCCCAGCCGGTCGCCTGCGCACCGGCGACGCATCGCT
GTACACGTATCTGCGGCGCAGCGTCCTCGACTTCGACGGGGCTTCGGCATTCCGGGACAGGTTGCAGCGCAACGGATTCA
CCGACGTCCACAGTGAGACCATGCCCGGTTGGCAGCGCAACGTCGTGCACACGTTCACCGGGAGGGCAACCCGATGA

Upstream 100 bases:

>100_bases
GTCGAGGACTTCCTGTTCGGTTTCGCGTTGGTGACCGGGGTGCTGCTGCTCTGGGAGCGTCAGCGGACCAGAGGCGTCAA
GGTCGGCGAGCGGGTGCACG

Downstream 100 bases:

>100_bases
AGGACCCCCGACGCGTCGTCCATCCGGCGTCCCCGGGACTACCGGACGCAACCTCGCTGACCACCCGCCCACGCGCGGTG
GTGGTCGGCGGGGGTATCGC

Product: type 12 methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSDALPHAQVPAAFDVGAAAYDKLVGANPGYHDHLRLSAQRMGLPDGGRGMRLLDAGCGTGASTAALLAAAPHAEIVAVD
ASAGMLAEARSKAWPGSVRFVHSRIEDIADADVTGPFDGIFAAYLLRNLADPDTQLRTFRDMLNPGAPLTVHEYSVRDSA
VATAVWNAVCWAIIIPAGRLRTGDASLYTYLRRSVLDFDGASAFRDRLQRNGFTDVHSETMPGWQRNVVHTFTGRATR

Sequences:

>Translated_238_residues
MSDALPHAQVPAAFDVGAAAYDKLVGANPGYHDHLRLSAQRMGLPDGGRGMRLLDAGCGTGASTAALLAAAPHAEIVAVD
ASAGMLAEARSKAWPGSVRFVHSRIEDIADADVTGPFDGIFAAYLLRNLADPDTQLRTFRDMLNPGAPLTVHEYSVRDSA
VATAVWNAVCWAIIIPAGRLRTGDASLYTYLRRSVLDFDGASAFRDRLQRNGFTDVHSETMPGWQRNVVHTFTGRATR
>Mature_237_residues
SDALPHAQVPAAFDVGAAAYDKLVGANPGYHDHLRLSAQRMGLPDGGRGMRLLDAGCGTGASTAALLAAAPHAEIVAVDA
SAGMLAEARSKAWPGSVRFVHSRIEDIADADVTGPFDGIFAAYLLRNLADPDTQLRTFRDMLNPGAPLTVHEYSVRDSAV
ATAVWNAVCWAIIIPAGRLRTGDASLYTYLRRSVLDFDGASAFRDRLQRNGFTDVHSETMPGWQRNVVHTFTGRATR

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=173, Percent_Identity=29.4797687861272, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25503; Mature: 25372

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALPHAQVPAAFDVGAAAYDKLVGANPGYHDHLRLSAQRMGLPDGGRGMRLLDAGCGT
CCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCC
GASTAALLAAAPHAEIVAVDASAGMLAEARSKAWPGSVRFVHSRIEDIADADVTGPFDGI
CHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
FAAYLLRNLADPDTQLRTFRDMLNPGAPLTVHEYSVRDSAVATAVWNAVCWAIIIPAGRL
HHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
RTGDASLYTYLRRSVLDFDGASAFRDRLQRNGFTDVHSETMPGWQRNVVHTFTGRATR
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHCEEECCCCCCCC
>Mature Secondary Structure 
SDALPHAQVPAAFDVGAAAYDKLVGANPGYHDHLRLSAQRMGLPDGGRGMRLLDAGCGT
CCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCC
GASTAALLAAAPHAEIVAVDASAGMLAEARSKAWPGSVRFVHSRIEDIADADVTGPFDGI
CHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
FAAYLLRNLADPDTQLRTFRDMLNPGAPLTVHEYSVRDSAVATAVWNAVCWAIIIPAGRL
HHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
RTGDASLYTYLRRSVLDFDGASAFRDRLQRNGFTDVHSETMPGWQRNVVHTFTGRATR
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]