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The map label for this gene is echA8 [H]

Identifier: 119871101

GI number: 119871101

Start: 5310354

End: 5311169

Strand: Direct

Name: echA8 [H]

Synonym: Mkms_5074

Alternate gene names: 119871101

Gene position: 5310354-5311169 (Clockwise)

Preceding gene: 119871100

Following gene: 119871103

Centisome position: 92.56

GC content: 68.5

Gene sequence:

>816_bases
ATGGGCGATACATACGAGTCGGTGACCATCGAGACCGACGGCCACGTCGCACAGGTGACGTTGATCGGCCCCGGTAAGGG
CAACGCGATGGGACCGGCGTTCTGGGCCGAACTGCCCGACGTGTTCACCTCGCTCGACGCCGACCCCGATGTGCGCGCCA
TCGTGCTCACCGGATCGGGCCGCAACTTCAGCTACGGCCTCGACCTGGCGGCCATGGGCGGCACGCTGCCCGGCCTCGAC
GCCGGCGCCCGATCACGGGCCGATTTCCACAAGACGCTGCAGAAGATGCAGGGGGCGATCAGCGCCGTCGCCGATTGCCG
CACCCCCACGGTCGCCTCGGTACACGGATGGTGCATCGGCGGCGGCGTCGACCTGATCAGCGCGGTGGACATCCGCTACG
CCAGCGCCGACGCCAAATTCTCGGTGCGTGAGGTCAAGCTCGCGATCGTCGCCGACGTCGGCTCGCTGGCCCGGCTGCCG
CTGATCCTGACCGACGGCCATCTCCGCGAACTCGCCCTGACCGGCAAGGACATCGACGCCGCGCGTGCCGAGAAGATCGG
TCTGGTCAACGACGTGTACGACGATCCGGAGGCCTCGCTGGCGGCCGCGCACGCCACCGCCAAGGAGATCGCGGCGAACC
CGCCGCTGACCGTGCACGGCGTCAAGGACGTGCTCGATCAGCAGCGCATCGCCAGGGTGTCGGAGAGCCTGCGCTATGTG
GCGGCGTGGAACTCGGCGTTCCTGCCGTCCAAGGACCTCGGCGAAGCCGTGACCGCGATGTTCCAGAAGCGGCCACCGAA
CTTCACCGGCGAATAG

Upstream 100 bases:

>100_bases
AGCGCACCGTCGTCGCCAAGACCCTGGCCGACGCGATCTGCGAGGTGCTGCCCTCGGCCAAGCTCAGCGACATCCTGATC
GGGCGCTAGCCTCGAACACC

Downstream 100 bases:

>100_bases
CTTTCGAGAGCGGTGGCGGAGGGATTTGAACCCCCGGACGGTGTTAGCCGTCTCTCGCTTTCAAGGCGAGTGCATTAGGC
CGCTCTGCCACGCCACCGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MGDTYESVTIETDGHVAQVTLIGPGKGNAMGPAFWAELPDVFTSLDADPDVRAIVLTGSGRNFSYGLDLAAMGGTLPGLD
AGARSRADFHKTLQKMQGAISAVADCRTPTVASVHGWCIGGGVDLISAVDIRYASADAKFSVREVKLAIVADVGSLARLP
LILTDGHLRELALTGKDIDAARAEKIGLVNDVYDDPEASLAAAHATAKEIAANPPLTVHGVKDVLDQQRIARVSESLRYV
AAWNSAFLPSKDLGEAVTAMFQKRPPNFTGE

Sequences:

>Translated_271_residues
MGDTYESVTIETDGHVAQVTLIGPGKGNAMGPAFWAELPDVFTSLDADPDVRAIVLTGSGRNFSYGLDLAAMGGTLPGLD
AGARSRADFHKTLQKMQGAISAVADCRTPTVASVHGWCIGGGVDLISAVDIRYASADAKFSVREVKLAIVADVGSLARLP
LILTDGHLRELALTGKDIDAARAEKIGLVNDVYDDPEASLAAAHATAKEIAANPPLTVHGVKDVLDQQRIARVSESLRYV
AAWNSAFLPSKDLGEAVTAMFQKRPPNFTGE
>Mature_270_residues
GDTYESVTIETDGHVAQVTLIGPGKGNAMGPAFWAELPDVFTSLDADPDVRAIVLTGSGRNFSYGLDLAAMGGTLPGLDA
GARSRADFHKTLQKMQGAISAVADCRTPTVASVHGWCIGGGVDLISAVDIRYASADAKFSVREVKLAIVADVGSLARLPL
ILTDGHLRELALTGKDIDAARAEKIGLVNDVYDDPEASLAAAHATAKEIAANPPLTVHGVKDVLDQQRIARVSESLRYVA
AWNSAFLPSKDLGEAVTAMFQKRPPNFTGE

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=265, Percent_Identity=39.622641509434, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI194097323, Length=270, Percent_Identity=27.037037037037, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4502327, Length=248, Percent_Identity=26.6129032258064, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=22.8070175438596, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=22.8070175438596, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI221142681, Length=271, Percent_Identity=30.2583025830258, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=25.7575757575758, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788682, Length=209, Percent_Identity=28.7081339712919, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=27.2388059701493, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=27.683615819209, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082183, Length=276, Percent_Identity=25.7246376811594, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=277, Percent_Identity=35.0180505415162, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=33.2075471698113, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17554946, Length=267, Percent_Identity=28.4644194756554, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=27.34375, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=202, Percent_Identity=29.2079207920792, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=210, Percent_Identity=30, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=215, Percent_Identity=26.9767441860465, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=188, Percent_Identity=31.9148936170213, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=188, Percent_Identity=31.9148936170213, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=244, Percent_Identity=29.9180327868852, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=38.8888888888889, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI20129971, Length=276, Percent_Identity=28.9855072463768, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24653477, Length=276, Percent_Identity=28.9855072463768, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24653139, Length=277, Percent_Identity=29.2418772563177, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24654903, Length=193, Percent_Identity=25.9067357512953, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28496; Mature: 28364

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDTYESVTIETDGHVAQVTLIGPGKGNAMGPAFWAELPDVFTSLDADPDVRAIVLTGSG
CCCCCCEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCC
RNFSYGLDLAAMGGTLPGLDAGARSRADFHKTLQKMQGAISAVADCRTPTVASVHGWCIG
CCEEECCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
GGVDLISAVDIRYASADAKFSVREVKLAIVADVGSLARLPLILTDGHLRELALTGKDIDA
CCHHHHHHHHEEEECCCCCEEHHEEEEEEEECCCCHHHCCEEEECCCEEEEEECCCCCCH
ARAEKIGLVNDVYDDPEASLAAAHATAKEIAANPPLTVHGVKDVLDQQRIARVSESLRYV
HHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHEEE
AAWNSAFLPSKDLGEAVTAMFQKRPPNFTGE
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GDTYESVTIETDGHVAQVTLIGPGKGNAMGPAFWAELPDVFTSLDADPDVRAIVLTGSG
CCCCCEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCC
RNFSYGLDLAAMGGTLPGLDAGARSRADFHKTLQKMQGAISAVADCRTPTVASVHGWCIG
CCEEECCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
GGVDLISAVDIRYASADAKFSVREVKLAIVADVGSLARLPLILTDGHLRELALTGKDIDA
CCHHHHHHHHEEEECCCCCEEHHEEEEEEEECCCCHHHCCEEEECCCEEEEEECCCCCCH
ARAEKIGLVNDVYDDPEASLAAAHATAKEIAANPPLTVHGVKDVLDQQRIARVSESLRYV
HHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHEEE
AAWNSAFLPSKDLGEAVTAMFQKRPPNFTGE
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]