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The map label for this gene is shiA [H]

Identifier: 119870991

GI number: 119870991

Start: 5203405

End: 5204724

Strand: Direct

Name: shiA [H]

Synonym: Mkms_4964

Alternate gene names: 119870991

Gene position: 5203405-5204724 (Clockwise)

Preceding gene: 119870990

Following gene: 119870992

Centisome position: 90.7

GC content: 68.71

Gene sequence:

>1320_bases
GTGACCATCGCCGACCCGCCGATCGCCCCGGGACCCGACGCAACCGACGACCGCCGGGTGCGCACCGCGCTGCTGTCCAG
CCTGGTCGGCACGACGATCGAGTGGTACGACTTCTTCCTCTACGCCACCGCCGCCAGCCTGGTGTTCAACCAGGCGTTCT
TCCCCGACCAGAGCTCTTTCGTCGGCACCATGCTGTCGTTCGCGACATTCGCCGTCGGGTTCGTCGTGCGGCCGATCGGC
GGATTCGTGTTCGGGCACATCGGTGACCGCATCGGCCGCAAACGCACGCTGGCGCTGACGATGTTCCTGATGGGCGGTGC
CACCGCGCTGATGGGCGTCCTGCCGACGGCAGCGCAGATCGGCGTGCTGGCACCGATCCTGCTGTTGCTGCTGCGCATCG
CGCAGGGGTTCGCGCTGGGCGGCGAATGGGCCGGGGCGGTGCTGCTGGCGGTCGAGCACAGTTCGGCGAAGCGCCGCGGC
TTCTCGGGCAGCGTCCCGCAGATCGGGCTGGCCCTCGGGCTGGCGCTGGGCACCGGGGTGTTCGCGCTGCTGCAGGTCGT
GCTGCCCGACGACGCGTTCCTGTCCTACGGCTGGCGAATCGCGTTCCTGGTCAGCGTGGTGCTGGTGATCTTCGGTGTGG
TGGTGCGCCTCAAGGCGGAGGAGACGCCGGCGTTCGAGAAGGTCCGCGACGACGATCAGCGGTCGGCCGTGCCGGTCAAG
GAGATCTTCCGGCCGCCGGTGCTCAAGTCGACGGTGCTGGGCATGCTGTCCCGCTGGGGCGAGGGCGCCGCGTTCAACAC
CTGGGGTGTCTTCGCGATCTCGTACGCGACGGCGACGCTGCACTTCGAGAAGGTGCCGGTGCTGATCGTGGTGACCGCGT
CGGCGTTGCTGATGGCCGTGTTGCTGCCGGTCTCGGGTCTGCTCGTCGACCGGTTCGGCGCCAAACGCGTCTACGGCACC
GGCATCGCCGCCTACGCGGTCGCGGTGTTCCCGGTGTTCGCGCTCTTCAACACCGGCAGCATGGTCACCTACACCGTCGG
GATGGTGGTGGTGTTCGGGGTCATCCACGCCTGGTTCTACGGCGCCCAGGGCACGCTGTACGCGTCGCTGTTCCCGACCA
GGATCCGGTACACCGGCCTGTCGACGGTCTATCAGCTCTCCGGGGTGTACTCGTCGGGTCTGACGCCGCTGATCCTCACC
GCGTTGATCGCCGCCGCGGGCAACACGCCGTGGTTGGCGTGCGGCTACCTGGTCGTGACCGCGGTGATCAGTGTCGTCGC
CACGGCGTTGCTGCGGCCGCGGGCACTGGCCGAACTCTAA

Upstream 100 bases:

>100_bases
TTCAGCGACCAGGTGCTCGTCGCGAGCAGGCCCCGCCACTAAGAATCACGACGCGCACAACTGTGGTGCGATCGCGCCGG
TTCGGGGCTAATGGGCCAAC

Downstream 100 bases:

>100_bases
AGGCGTGCGGGCGCCGAACGAGCGAATACCTGCGCGGGCGTCACTAGCATCGGCTGAGTGGGCGAAGAAGTGAAGCGGAC
CGACTTCAGCCATGCGCAGC

Product: major facilitator superfamily transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTIADPPIAPGPDATDDRRVRTALLSSLVGTTIEWYDFFLYATAASLVFNQAFFPDQSSFVGTMLSFATFAVGFVVRPIG
GFVFGHIGDRIGRKRTLALTMFLMGGATALMGVLPTAAQIGVLAPILLLLLRIAQGFALGGEWAGAVLLAVEHSSAKRRG
FSGSVPQIGLALGLALGTGVFALLQVVLPDDAFLSYGWRIAFLVSVVLVIFGVVVRLKAEETPAFEKVRDDDQRSAVPVK
EIFRPPVLKSTVLGMLSRWGEGAAFNTWGVFAISYATATLHFEKVPVLIVVTASALLMAVLLPVSGLLVDRFGAKRVYGT
GIAAYAVAVFPVFALFNTGSMVTYTVGMVVVFGVIHAWFYGAQGTLYASLFPTRIRYTGLSTVYQLSGVYSSGLTPLILT
ALIAAAGNTPWLACGYLVVTAVISVVATALLRPRALAEL

Sequences:

>Translated_439_residues
MTIADPPIAPGPDATDDRRVRTALLSSLVGTTIEWYDFFLYATAASLVFNQAFFPDQSSFVGTMLSFATFAVGFVVRPIG
GFVFGHIGDRIGRKRTLALTMFLMGGATALMGVLPTAAQIGVLAPILLLLLRIAQGFALGGEWAGAVLLAVEHSSAKRRG
FSGSVPQIGLALGLALGTGVFALLQVVLPDDAFLSYGWRIAFLVSVVLVIFGVVVRLKAEETPAFEKVRDDDQRSAVPVK
EIFRPPVLKSTVLGMLSRWGEGAAFNTWGVFAISYATATLHFEKVPVLIVVTASALLMAVLLPVSGLLVDRFGAKRVYGT
GIAAYAVAVFPVFALFNTGSMVTYTVGMVVVFGVIHAWFYGAQGTLYASLFPTRIRYTGLSTVYQLSGVYSSGLTPLILT
ALIAAAGNTPWLACGYLVVTAVISVVATALLRPRALAEL
>Mature_438_residues
TIADPPIAPGPDATDDRRVRTALLSSLVGTTIEWYDFFLYATAASLVFNQAFFPDQSSFVGTMLSFATFAVGFVVRPIGG
FVFGHIGDRIGRKRTLALTMFLMGGATALMGVLPTAAQIGVLAPILLLLLRIAQGFALGGEWAGAVLLAVEHSSAKRRGF
SGSVPQIGLALGLALGTGVFALLQVVLPDDAFLSYGWRIAFLVSVVLVIFGVVVRLKAEETPAFEKVRDDDQRSAVPVKE
IFRPPVLKSTVLGMLSRWGEGAAFNTWGVFAISYATATLHFEKVPVLIVVTASALLMAVLLPVSGLLVDRFGAKRVYGTG
IAAYAVAVFPVFALFNTGSMVTYTVGMVVVFGVIHAWFYGAQGTLYASLFPTRIRYTGLSTVYQLSGVYSSGLTPLILTA
LIAAAGNTPWLACGYLVVTAVISVVATALLRPRALAEL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=427, Percent_Identity=38.1733021077283, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1790550, Length=431, Percent_Identity=32.7146171693735, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1789941, Length=393, Percent_Identity=37.6590330788804, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788942, Length=424, Percent_Identity=26.8867924528302, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI87082404, Length=404, Percent_Identity=23.2673267326733, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46586; Mature: 46455

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIADPPIAPGPDATDDRRVRTALLSSLVGTTIEWYDFFLYATAASLVFNQAFFPDQSSF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VGTMLSFATFAVGFVVRPIGGFVFGHIGDRIGRKRTLALTMFLMGGATALMGVLPTAAQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLAPILLLLLRIAQGFALGGEWAGAVLLAVEHSSAKRRGFSGSVPQIGLALGLALGTGV
HHHHHHHHHHHHHHCCCCCCCCHHCEEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHH
FALLQVVLPDDAFLSYGWRIAFLVSVVLVIFGVVVRLKAEETPAFEKVRDDDQRSAVPVK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHH
EIFRPPVLKSTVLGMLSRWGEGAAFNTWGVFAISYATATLHFEKVPVLIVVTASALLMAV
HHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCEEEEHHHHHHHHHH
LLPVSGLLVDRFGAKRVYGTGIAAYAVAVFPVFALFNTGSMVTYTVGMVVVFGVIHAWFY
HHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GAQGTLYASLFPTRIRYTGLSTVYQLSGVYSSGLTPLILTALIAAAGNTPWLACGYLVVT
CCCCCEEEECCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
AVISVVATALLRPRALAEL
HHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
TIADPPIAPGPDATDDRRVRTALLSSLVGTTIEWYDFFLYATAASLVFNQAFFPDQSSF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VGTMLSFATFAVGFVVRPIGGFVFGHIGDRIGRKRTLALTMFLMGGATALMGVLPTAAQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLAPILLLLLRIAQGFALGGEWAGAVLLAVEHSSAKRRGFSGSVPQIGLALGLALGTGV
HHHHHHHHHHHHHHCCCCCCCCHHCEEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHH
FALLQVVLPDDAFLSYGWRIAFLVSVVLVIFGVVVRLKAEETPAFEKVRDDDQRSAVPVK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHH
EIFRPPVLKSTVLGMLSRWGEGAAFNTWGVFAISYATATLHFEKVPVLIVVTASALLMAV
HHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCEEEEHHHHHHHHHH
LLPVSGLLVDRFGAKRVYGTGIAAYAVAVFPVFALFNTGSMVTYTVGMVVVFGVIHAWFY
HHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GAQGTLYASLFPTRIRYTGLSTVYQLSGVYSSGLTPLILTALIAAAGNTPWLACGYLVVT
CCCCCEEEECCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
AVISVVATALLRPRALAEL
HHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]